Ibudilast-Induced Decreases in Cytosolic Ca2+ Level and Contraction in Rat Aorta

Kaneda, Toshio; Konno, Yasushi; Urakawa, Norimoto; Nakajyo, Shinjiro; Shimizu, Kazumasa

doi:10.1254/jphs.fp0070073

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…In the endothelium-intact aorta, cGMP content was measured by enzyme immunoassay, as reported previously [8] . The aorta was incubated with PE (1 μmol/l) for 15 min and then DMSO or CCh were added for 5 min.…”

Section: Assay Of Cgmp Contentmentioning

confidence: 99%

Endothelium-Dependent and -Independent Vasodilator Effects of Dimethyl Sulfoxide in Rat Aorta

Kaneda

Sasaki²,

Urakawa³

et al. 2016

Pharmacology

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This study examined the mechanism of vasorelaxation induced by dimethyl sulfoxide (DMSO) in endothelium-intact and -denuded rat aorta. DMSO (0.1-3%) inhibited phenylephrine (PE, 1 μmol/l)-induced contraction in a dose-dependent manner. However, this relaxation was lower in the absence of the endothelium. Increase in DMSO-induced relaxation in the presence of the endothelium was attenuated by preincubation in L-N^G-nitroarginine methyl ester (L-NAME, 100 μmol/l) and by the removal of the endothelium. In the aorta with endothelium, DMSO (3%) and CCh (3 μmol/l) increased cGMP contents, significantly and L-NAME (100 μmol/l) inhibited the DMSO-induced increases of cGMP. In fura 2-loaded endothelium-denuded aorta, cumulative application of DMSO (1-3%) inhibited PE-induced muscle tension; however, this application did not affect the [Ca²⁺]_i level. In PE-precontracted endothelium-denuded aorta, relaxation responses to fasudil were significantly less in the presence of DMSO compared to the control. These results suggest that DMSO causes relaxation by increasing the cGMP content in correlation with the release of NO from endothelial cells and by decreasing the Ca²⁺ sensitivity of contractile elements partly via inhibiting Rho-kinase in rat aorta.

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Section: Assay Of Cgmp Contentmentioning

confidence: 99%

Endothelium-Dependent and -Independent Vasodilator Effects of Dimethyl Sulfoxide in Rat Aorta

Kaneda

Sasaki²,

Urakawa³

et al. 2016

Pharmacology

Self Cite

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show abstract

Antinociceptive, anti-inflammatory and smooth muscle relaxant activities of the pyrrolo[3,4-d]pyridazinone derivatives: Possible mechanisms of action

Mogilski

Kubacka

Redzicka

et al. 2015

Pharmacology Biochemistry and Behavior

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Ibudilast-Induced Decreases in Cytosolic Ca2+ Level and Contraction in Rat Aorta

Abstract: Abstract. The mechanism by which ibudilast induces vasodilation was examined in isolated endothelium-denuded rat aorta. Ibudilast inhibited the contractions induced by phenylephrine (PE) ] i level in endotheliumdenuded rat aorta.

Cited by 2 publications

References 22 publications

Endothelium-Dependent and -Independent Vasodilator Effects of Dimethyl Sulfoxide in Rat Aorta

Endothelium-Dependent and -Independent Vasodilator Effects of Dimethyl Sulfoxide in Rat Aorta

Antinociceptive, anti-inflammatory and smooth muscle relaxant activities of the pyrrolo[3,4-d]pyridazinone derivatives: Possible mechanisms of action

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