2015
DOI: 10.1039/c5cp00272a
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Ibuprofen causes photocleavage through ROS generation and intercalates with DNA: a combined biophysical and molecular docking approach

Abstract: Ibuprofen is an important nonsteroidal anti-inflammatory drug endowed with various pharmacological and biological activities. In the present study, the photochemical properties of ibuprofen were evaluated by assaying the generation of various reactive oxygen species (ROS) such as superoxide, singlet oxygen and the hydroxyl radical. ROS generated by ibuprofen in the presence of white light causes DNA strand scission as observed by plasmid nicking assay. Ibuprofen induced ROS generation is also capable of causin… Show more

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Cited by 71 publications
(41 citation statements)
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“…Ibuprofen can cause the enhanced generation of reactive oxygen species and leads to DNA damages, protein carboxylation, and lipid peroxidation (Gómez-Oliván et al, 2014; Husain et al, 2015). Ibuprofen decreases quality of sperm in mice and its chromatin/DNA integrity (Roodbari et al, 2015).…”
Section: Discussionmentioning
confidence: 99%
“…Ibuprofen can cause the enhanced generation of reactive oxygen species and leads to DNA damages, protein carboxylation, and lipid peroxidation (Gómez-Oliván et al, 2014; Husain et al, 2015). Ibuprofen decreases quality of sperm in mice and its chromatin/DNA integrity (Roodbari et al, 2015).…”
Section: Discussionmentioning
confidence: 99%
“…The intercalation mode of binding of small molecules to the DNA double-helix results in an increase of the length of the double-helix due to an increase in the base pair separations, which in turn results in an increase in the viscosity [62]. However, in the case of electrostatic interactions and groove binding, no such enhancement of the viscosity of DNA is observed [63].…”
Section: Viscosity Measurementsmentioning
confidence: 99%
“…Molecular modelling programs are commonly employed in determining the binding mode of various drugs where intercalators and groove binders are easily distinguished (Fig. 9) [5,25,26,41,42,51]. The beginning step of docking process involves retrieving the structure of the receptor or macromolecule (DNA) from Protein Data Bank (PDB) available at http://www.rcsb.org/ pdb.…”
Section: Molecular Dockingmentioning
confidence: 99%
“…Fluorescence spectroscopy also finds application in deducing various thermodynamic parameters involved in the interaction of small molecules with DNA [25,26]. Hydrogen bonding, van der Waals forces, hydrophobic interactions and electrostatic interactions are the four major non-covalent interactions that play an important role in drug-DNA interaction.…”
Section: Fluorescence Spectroscopymentioning
confidence: 99%