Ibuprofen-mediated potential inhibition of biofilm development and quorum sensing in Pseudomonas aeruginosa

Dai, Lu; TianQi, Wu; Xiong, Yi-song; Ni, Hongbing; Ding, Ye; Zhang, Wen-chen; Chu, Shao-peng; Ju, Songguang; Yu, Jian

doi:10.1016/j.lfs.2019.116947

Cited by 50 publications

(43 citation statements)

References 48 publications

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“…In recent times, compounds currently used for the treatment of noninfectious diseases as potential antimicrobial alternatives have also been reviewed (Lagadinou et al, 2020;Zimmermann & Curtis, 2017). One example is Ibuprofen, a nonsteroidal anti-inflammatory drug (NSAID) widely used for relief of pain, fever and inflammation, which has demonstrated novel pharmacological actions against bacteria (Dai et al, 2019;Obad et al, 2015;Shah et al, 2018), fungi (Ogundeji et al, 2016;Sanyal et al, 1993) and viruses (Pan et al, 2018;Veljkovic et al, 2015). Interestingly, a short time ago, it was reported a nebulizable formulation of sodium Ibuprofen in high ionic strength with bactericidal properties for the treatment of respiratory infections of fibrotic patients (Muñoz et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Anin silicoanalysis of Ibuprofen enantiomers in high concentrations of sodium chloride with SARS-CoV-2 main protease

Clemente

Freiberger

Ravetti

et al. 2021

Journal of Biomolecular Structure and Dynamics

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2020 will be remembered worldwide for the outbreak of Coronavirus disease (COVID-19), which quickly spread until it was declared as a global pandemic. The main protease (Mpro) of SARS-CoV-2, a key enzyme in coronavirus, represents an attractive pharmacological target for inhibition of SARS-CoV-2 replication. Here, we evaluated whether the anti-inflammatory drug Ibuprofen, may act as a potential SARS-CoV-2 Mpro inhibitor, using an in silico study. From molecular dynamics (MD) simulations, we also evaluated the influence of ionic strength on the affinity and stability of the Ibuprofen-Mpro complexes. The docking analysis shows that R(À)Ibuprofen and S(þ)Ibuprofen isomers can interact with multiple key residues of the main protease, through hydrophobic interactions and hydrogen bonds, with favourable binding energies (À6.2 and À5.7 kcal/mol, respectively). MM-GBSA and MM-PBSA calculations confirm the affinity of these complexes, in terms of binding energies. It also demonstrates that the ionic strength modifies significantly their binding affinities. Different structural parameters calculated from the MD simulations (120 ns) reveal that these complexes are conformational stable in the different conditions analysed. In this context, the results suggest that the condition 2 (0.25 NaCl) bind more tightly the Ibuprofen to Mpro than the others conditions. From the frustration analysis, we could characterize two important regions (Cys44-Pro52 and Linker loop) of this protein involved in the interaction with Ibuprofen. In conclusion, our findings allow us to propose that racemic mixtures of the Ibuprofen enantiomers might be a potential treatment option against SARS-CoV-2 Mpro. However, further research is necessary to determinate their possible medicinal use.

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Section: Introductionmentioning

confidence: 99%

Anin silicoanalysis of Ibuprofen enantiomers in high concentrations of sodium chloride with SARS-CoV-2 main protease

Clemente

Freiberger

Ravetti

et al. 2021

Journal of Biomolecular Structure and Dynamics

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“…Also, azithromycin interferes with QS, reducing gene expression and the production of autoinducers in Pseudomonas aeruginosa, while streptomycin does so in Acinetobacter baumannii (Nalca et al, 2006;Saroj and Rather, 2013). Interestingly, this phenomenon has also been identified in drugs of mass consumption such as aspirin (El-Mowafy et al, 2014) and ibuprofen (Dai et al, 2019), in fermented products, and in various bactericidal phytochemicals (Muñoz-Cazares et al, 2017). Thus, the effect of metabolites at low concentrations on microbial social networks and virulence regulation is a frontier issue that increases the number of molecules to be explored at sub-inhibitory concentrations (Figure 1).…”

Section: Anti-virulence Properties Of Bactericidal Phytochemicalsmentioning

confidence: 99%

The New Antibacterial Properties of the Plants: Quo vadis Studies of Anti-virulence Phytochemicals?

2021

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The recent increase in bacterial resistance to antibiotics has motivated the resurgence of the study of natural antimicrobial products. For centuries, plants have been recognized for their bactericidal properties. However, in the last two decades, it has been reported that several plant derived metabolites at growth subinhibitory concentrations also tend to have anti-virulence properties, since they reduce the expression of factors that cause damage and the establishment of pathogenic bacteria. In this area of study, plants have been positioned as one of the main natural sources of anti-virulence molecules, but only a small portion of the plant species that exist have been investigated. Also, anti-virulence studies have been primarily focused on analyzing the ability of extracts and compounds to inhibit quorum sensing and biofilms formation in vitro. This mini-review discusses the current panorama, the trends in the study of anti-virulence phytochemicals, as well as their potential for the development of antibacterial therapies.

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“…Unfortunately, many common community-acquired pathogenic bacteria, such as Escherichia coli, Klebsiella pneumoniae, Salmonella , methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa , have shown progressive resistance to conventional drugs. A World Health Organisation (WHO) report in 2019 concluded that if the current trend is not averted drug-resistant diseases could lead to the death of 10 million people each year by 2050 (Raymond and Powell 2019; Dai et al . 2019).…”

Section: Introductionmentioning

confidence: 99%

“…As such, interference of the QS system in pathogenic bacteria represents an attractive target for the development of novel therapeutics. For instance, the use of small molecules as potential inhibitors against QS mediated genes responsible for virulence determinants and biofilm formation is an effective strategy in combating microbial resistance to conventional antibiotics (Dai et al . 2019).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Quorum Sensing System inPseudomonas aeruginosaby Psammaplin A and Bisaprasin Isolated from the Marine SpongeAplysinella rhax

Oluwabusola

et al. 2021

Preprint

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Natural products isolated from marine sponges have exhibited profound bioactivity and, in some cases, serve as potent quorum sensing inhibitory agents by preventing microbial biofilm formation. In this study, the inhibitory potential of the psammaplin type compounds, psammaplin A (1) and bisaprasin (2), isolated from the marine sponge, Aplysinella rhax, was evaluated in the quorum-sensing inhibitory assay based on the Pseudomonas aeruginosa PAO1 lasB-gfp and P. aeruginosa PAO1 rhlA-gfp biosensor strains. The result indicated that psammaplin A (1) showed moderate inhibition against lasB-gfp biosensor strains but significantly inhibited the QS-gene promoters, rhlA-gfp with IC50 value at 30.69 and 2.64 μM, respectively. In contrast, bisaprasin (2) displayed significant inhibition for both biosensor strains, lasB-gfp and rhlA-gfp with IC50 values at 8.70 and 8.53 μM, respectively. To our knowledge, the antiquorum sensing activity of these marine-derived bromotyrosine compounds is described here for the first time.Significance and Impact of the StudyThe attention of the scientific community has been drawn to using marine sources to find novel quorum sensing inhibitors as antipathogenic drugs to combat antimicrobial resistance and pathogenesis caused by the proliferation of pathogenic bacteria mediated by the quorum sensing (QS) system. By blocking the QS signalling communication, the ability to assemble an organised community structure that enables drug resistance and production of virulence factors will be attenuated. The significance of this investigation is based on the discovery of bromotyrosine derivatives as potential new drug leads for the development of antipathogenic agents.

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Ibuprofen-mediated potential inhibition of biofilm development and quorum sensing in Pseudomonas aeruginosa

Cited by 50 publications

References 48 publications

Anin silicoanalysis of Ibuprofen enantiomers in high concentrations of sodium chloride with SARS-CoV-2 main protease

Anin silicoanalysis of Ibuprofen enantiomers in high concentrations of sodium chloride with SARS-CoV-2 main protease

The New Antibacterial Properties of the Plants: Quo vadis Studies of Anti-virulence Phytochemicals?

Inhibition of Quorum Sensing System inPseudomonas aeruginosaby Psammaplin A and Bisaprasin Isolated from the Marine SpongeAplysinella rhax

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