ICAM-1 regulates neutrophil adhesion and transcellular migration of TNF-α-activated vascular endothelium under flow

Abstract

In vivo, leukocyte transendothelial migration (TEM) occurs at endothelial cell junctions (paracellular) and nonjunctional (transcellular) locations, whereas in vitro models report that TEM is mostly paracellular. The mechanisms that control the route of leukocyte TEM remain unknown. IntroductionLeukocyte transendothelial migration (TEM) is a key step in their recruitment to sites of inflammation, injury, and immune reactions. Leukocyte recruitment involves a multistep cascade consisting of leukocyte rolling, firm adhesion, and, ultimately, transmigration. 1 These steps are regulated by shear flow, apical chemokines, and inducible adhesion molecules expressed by endothelium. 2 The actual path of leukocyte egress (paracellular versus transcellular) has been examined in several models (reviewed in Muller, 3 Kvietys and Sandig, 4 and Engelhardt and Wolburg 5 ). Experiments carried out in several animal models of inflammation found that leukocyte TEM can occur at both paracellular and transcellular locations. [6][7][8][9][10][11][12] In contrast, most in vitro studies have reported that leukocyte TEM occurs primarily at paracellular locations. 3,[13][14][15] As a result, in vitro models of polymorphonuclear leukocyte (PMN) transcellular TEM do not exist, and the factors that dictate whether leukocytes use a transcellular or paracellular route and the mechanisms underlying transcellular TEM are unknown.Intercellular adhesion molecule-1 (ICAM-1) interacting with its leukocyte counterreceptors lymphocyte function-associated antigen-1 (LFA-1) and macrophage antigen-1 (Mac-1) is key for leukocyte adhesion and TEM. [16][17][18][19] ICAM-1 is a transmembrane glycoprotein with 5 extracellular immunoglobulin G (IgG)-like domains and a short cytoplasmic tail that associates with multiple cytoskeletal linker proteins (reviewed in Springer 1 ). Vascular endothelium expresses low levels of ICAM-1, and inflammatory stimuli can markedly increase ICAM-1 surface expression. 20,21 In acute and chronic inflammatory diseases, endothelial cells become activated and express high levels of ICAM-1, in addition to vascular cell adhesion molecule-1 (VCAM-1) and E-selectin. [22][23][24][25] ICAM-1 has been shown to signal in endothelium during leukocyte adhesion or when cross-linked by anti-ICAM-1 antibodies. ICAM-1 occupancy triggers elevations in intracellular free Ca 2ϩ and myosin contractility, 26 activation of p38 kinase, 27 and the small guanosine triphosphatases (GTPases), in particular, members of the Rho family 28,29 and the tyrosine kinase p60 Src . 30 Activation of these signaling pathways results in extensive cytoskeletal remodeling events that alter endothelial cell contractility and function, 27 possibly facilitating leukocyte diapedesis. Recently, we have shown that neutrophil LFA-1 rapidly redistributes to form a ringlike structure that coclusters with endothelial ICAM-1 during PMN transmigration at cell junctions. 31 Others have reported that ICAM-1-enriched projections engulf leukocytes during their firm adhesion and locomot...

Intercellular adhesion molecule-1 is a regulator of blood–testis barrier function

“…2Ba-h). This is in agreement with previous reports that used other models (Millán et al, 2006;Yang et al, 2005), as well as with subsequent experiments that used adult testes for immunofluorescent staining. Here, ICAM-1 partially co-localized with occludin and ZO-1, as well as with N-cadherin and b-catenin at the BTB (Fig.…”

Dehydrated human amnion/chorion membrane regulates stem cell activity in vitro

“…Similarly ICAM‐1, which was upregulated in all three stem cell types, is an adhesion molecule that facilitates leukocyte endothelial transmigration,38 suggesting a potential role of ICAM‐1 in the extravasation of circulating stem cells from the blood vessels toward the dHACM treated site. In wound settings, these chemokines are commonly expressed early following establishment of hemostasis, often peaking at day 1 with expression sustained through the first week of healing, and have also been shown to promote angiogenesis 37…”

Leukocyte transendothelial migration: A local affair

“…Surface density and distribution of endothelial ICAM-1 induced a transition from paracellular to transcellular migration, while intermediate levels favored the paracellular route. 20,21 Related to the amount of surface ligands, neutrophil-EC interactions during TEM does increase integrin expression at the surface of neutrophils thereby affecting their activity and behavior after transmigration. 22 …”