Icariin immobilized electrospinning poly(l-lactide) fibrous membranes via polydopamine adhesive coating with enhanced cytocompatibility and osteogenic activity

Liu, Hua; Li, Wenling; Luo, Binghong; Chen, Xuexing; Wen, Wei; Zhou, Changren

doi:10.1016/j.msec.2017.05.077

Cited by 52 publications

(43 citation statements)

References 47 publications

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“…60 Improved hydrophilicity after modification may play a significant role in facilitating cell adhesion and proliferation. 57 The HA/CS multilayer alone could improve surface hydrophilicity; however, the incorporation of ICA decreased the hydrophilicity to some extent, and the wettability further decreased with increasing ICA concentration, similar to previous studies. 30 In our study, the HA/ CS-ICA-L surface showed the highest cell proliferation, while the HA/CS-ICA-M surface displayed the best differentiation ability.…”

Section: Discussionsupporting

confidence: 89%

“…ALP is regarded as one of the early markers of osteoblast differentiation and plays an important role in bone mineralization. 57 The ALP activity was particularly high in the HA/CS-ICA-M group, demonstrating the highest cell differentiation ability of a certain concentration. Similar to previous study, 42 the ALP activities of MC3T3-E1 cells after 14 days of culture on all HA/CS-ICA surfaces were lower than those after 7 days of culture, indicating that the cell response stage shifted from the differentiation period to the next mineralization stage.…”

Section: Discussionmentioning

confidence: 90%

“…It was observed that ICA loaded in scaffold was able to increase osteoblast proliferation at low doses (1.5×10 −5 mol/L group), while samples with high doses (6×10 −5 mol/L group) of ICA had lower proliferation activity. 57 As we know, the scaffold has a larger volume to load drugs, while the Ti implant has a smaller surface area and thickness to carry drugs. Therefore, it is reasonable to presume that the loaded drug concentration in the scaffold should be lower than that on the surface of the implant.…”

Section: Discussionmentioning

confidence: 99%

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Loading icariin on titanium surfaces by phase-transited lysozyme priming and layer-by-layer self-assembly of hyaluronic acid/chitosan to improve surface osteogenesis ability

Song¹,

Ma²,

Ning³

et al. 2018

IJN

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PurposeIcariin (ICA) is one of the main active constituents of Herba Epimedii for improving osteogenesis. It is necessary to create a simple and efficient method to load ICA onto the surface of titanium (Ti) implant. The purpose of this study was to establish a local ICA delivery system via a layer-by-layer (LbL) self-assembly system on phase-transited lysozyme (PTL)-primed Ti surface.Materials and methodsA PTL nanofilm was first firmly coated on the pristine Ti. Then, the ICA-loaded hyaluronic acid/chitosan (HA/CS) multilayer was applied via the LbL system to form the HA/CS-ICA surface. This established HA/CS-ICA surface was characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle measurement. The ICA release pattern of the HA/CS-ICA surface was also examined. MC3T3-E1 osteoblast culture test and a rat model were used to evaluate the effects of the HA/CS-ICA surface in vitro and in vivo.ResultsSEM, XPS and contact angle measurement demonstrated successful fabrication of the HA/CS-ICA surface. The HA/CS-ICA surfaces with different ICA concentrations revealed a controlled release profile of ICA during a 2-week monitoring span. Osteoblasts grown on the coated substrates displayed higher adhesion, viability, proliferation and ALP activity than those on the polished Ti surface. Furthermore, in vivo histological evaluation revealed much obvious bone formation in the ICA-coated group by histological staining and double fluorescent labeling at 2 weeks after implantation.ConclusionThe present study demonstrated that ICA-immobilized HA/CS multilayer on the PTL-primed Ti surface had a sustained release pattern of ICA which could promote the osteogenesis of osteoblasts in vitro and improve early osseointegration in vivo. This study provides a novel method for creating a sustained ICA delivery system to improve osteoblast response and osseointegration.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

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Loading icariin on titanium surfaces by phase-transited lysozyme priming and layer-by-layer self-assembly of hyaluronic acid/chitosan to improve surface osteogenesis ability

Song¹,

Ma²,

Ning³

et al. 2018

IJN

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“…As a result, the tensile strength, elongation at break, and Young's modulus of the PLGA‐PDA scaffolds were improved by 15.168, 18.751, and 42.414% over those of the PLGA scaffolds and reached 1.063 ± 0.054 MPa, 122.932 ± 6.412%, and 0.413 ± 0.004 GPa, respectively. Similar enhancements were observed by Liu et al . Additionally, the tensile strength of the scaffolds was further improved by immobilization of GO, attributed to the interfacial interactions between –OH or –NH 2 of PDA, the oxygen‐containing functional moieties (–OH and –COOH) of GO and the effective external load transfer of GO nanosheets.…”

Section: Resultssupporting

confidence: 83%

Graphene Oxide Immobilized PLGA‐polydopamine Nanofibrous Scaffolds for Growth Inhibition of Colon Cancer Cells

Chen

Jiang

Zhang

et al. 2018

Macromolecular Bioscience

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Graphene oxide (GO)/poly (lactide‐co‐glycolic acid) (PLGA) scaffolds have promising applications in the biomedical field. However, greater attention is focused on the incorporated system and its applications in normal cells. In this work, a novel GO immobilized PLGA nanofibrous scaffold assisted by polydopamine (PLGA‐PDA‐GO) is developed for growth inhibition of HT‐29 colon cancer cells. The interactions between GO and PDA are attributed to a π–π conjugate interaction and electrostatic attraction. In addition to the enhancement of thermal stability and mechanical strength, the surface roughness, hydrophilicity, and electro‐activity of the scaffolds are significantly improved by immobilization of GO. The scaffolds show good inhibition of HT‐29, and immobilized GO is observed to be in contact with but not internalized in HT‐29 cells. The cytotoxicity mechanism of scaffolds toward HT‐29 is attributed to intracellular activated reactive oxygen species that result from the physical interaction of the sharp GO edges and electrical signals of π–π stacking between PDA and GO.

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“…The surface water contact angle reects the hydrophilicity of the brous scaffolds, which will inuence protein absorption and subsequent cell adhesion on the membranes. 50,51 The effect of the immobilized PDA and OGP on the water contact angle of the brous scaffolds is shown in Fig. 1a and c. The PLLA scaffolds had the water contact angle of 122.5 AE 7.3 ( Fig.…”

Section: Physicochemical Properties Of the Scaffoldsmentioning

confidence: 99%

Polydopamine-modified poly(l-lactic acid) nanofiber scaffolds immobilized with an osteogenic growth peptide for bone tissue regeneration

Liu

et al. 2019

RSC Adv.

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Icariin immobilized electrospinning poly(l-lactide) fibrous membranes via polydopamine adhesive coating with enhanced cytocompatibility and osteogenic activity

Cited by 52 publications

References 47 publications

Loading icariin on titanium surfaces by phase-transited lysozyme priming and layer-by-layer self-assembly of hyaluronic acid/chitosan to improve surface osteogenesis ability

Loading icariin on titanium surfaces by phase-transited lysozyme priming and layer-by-layer self-assembly of hyaluronic acid/chitosan to improve surface osteogenesis ability

Graphene Oxide Immobilized PLGA‐polydopamine Nanofibrous Scaffolds for Growth Inhibition of Colon Cancer Cells

Polydopamine-modified poly(l-lactic acid) nanofiber scaffolds immobilized with an osteogenic growth peptide for bone tissue regeneration

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