Icariin inhibits TNF-α/IFN-γ induced inflammatory response via inhibition of the substance P and p38-MAPK signaling pathway in human keratinocytes

Kong, Lingwen; Liu, Jiaqi; Wang, Jia; Luo, Qingli; Zhang, Hongying; Liu, Baojun; Xu, Fei; Pang, Qi; Liu, Yingchao; Dong, Jingcheng

doi:10.1016/j.intimp.2015.10.023

Cited by 90 publications

(64 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mitogen activated protein kinases mainly act as regulators of transcription factors and transmitters that relay extracellular signals to the nucleus and further trigger target gene expression. Icariin inhibited TNF-a/IFN-c induced inflammatory response via inhibition of p38-MAPK signalling pathway in human keratinocytes [34]. Geniposide suppressed LPS-induced nitric oxide, PGE2 and inflammatory cytokine by downregulating MAPK and AP-1 signalling pathways in macrophages [35].…”

Section: Discussionmentioning

confidence: 99%

Thymus vulgaris alleviates UVB irradiation induced skin damage via inhibition of MAPK/AP‐1 and activation of Nrf2‐ARE antioxidant system

Sun

Park

Hwang

et al. 2016

J Cellular Molecular Medi

View full text Add to dashboard Cite

Solar ultraviolet (UV) radiation‐induced reactive oxidative species is mainly responsible for the development of photoageing. Rosmarinic acid was one of the main bioactive components detected in Thymus vulgaris (TV) we extracted. In this study, UVB‐induced skin damages have been shown to be ameliorated by treatment with TV in hairless mice (HR‐1) skin, demonstrated by decreased matrix metalloproteinases (MMPs) and increased collagen production. However, the underlying molecular mechanism on which TV acted was unclear. We examined the photoprotective effects of TV against UVB and elucidated the molecular mechanism in normal human dermal fibroblasts. Thymus vulgaris remarkably prevented the UVB‐induced reactive oxygen species and lactate dehydrogenase. Dose‐dependent increase in glutathione, NAD(P)H: quinone oxidoreductase1 and heme oxygenase‐1, by TV was confirmed by increased nuclear accumulation of Nrf2. Furthermore, 5‐Methoxyindole‐2‐carboxylic acid was introduced as a specific inhibitor of dihydrolipoyl dehydrogenase (DLD). We demonstrated that Nrf2 expression was regulated by DLD, which was a tricarboxylic acid cycle‐associated protein that decreased after UVB exposure. Besides, TV significantly diminished UVB induced phosphorylation of mitogen activated protein kinases pathway, containing extracellular signal‐regulated kinase, Jun N‐terminal kinase and p38, which consequently reduced phosphorylated c‐fos and c‐jun. Our results suggest that TV is a potential botanical agent for use against UV radiation‐induced oxidative stress mediated skin damages.

show abstract

Section: Discussionmentioning

confidence: 99%

Thymus vulgaris alleviates UVB irradiation induced skin damage via inhibition of MAPK/AP‐1 and activation of Nrf2‐ARE antioxidant system

Sun

Park

Hwang

et al. 2016

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…These Th2-related chemokines are considered to play an important role in developing atopic dermatitis [139,142]. Many researchers have attempted to inhibit the inflammatory cytokines and chemokines in TNF-α-and IFN-γ-stimulated keratinocytes and have analyzed the biological and pharmacological mechanisms with various factors [143][144][145].…”

Section: Atopic Dermatitismentioning

confidence: 99%

Therapeutic Effects of Apamin as a Bee Venom Component for Non-Neoplastic Disease

Han

Park

2020

Toxins

View full text Add to dashboard Cite

Bee venom is a natural toxin produced by honeybees and plays an important role in defending bee colonies. Bee venom has several kinds of peptides, including melittin, apamin, adolapamine, and mast cell degranulation peptides. Apamin accounts for about 2%-3% dry weight of bee venom and is a peptide neurotoxin that contains 18 amino acid residues that are tightly crosslinked by two disulfide bonds. It is well known for its pharmacological functions, which irreversibly block Ca2+-activated K+ (SK) channels. Apamin regulates gene expression in various signal transduction pathways involved in cell development. The aim of this study was to review the current understanding of apamin in the treatment of apoptosis, fibrosis, and central nervous system diseases, which are the pathological processes of various diseases. Apamin's potential therapeutic and pharmacological applications are also discussed.

show abstract

“…Cells were cultured in 36‐well plates (Corning Inc.), and viability was assessed using a commercial kit (Cell Counting Kit‐8, Dojindo Laboratories) in accordance with the manufacturer's instructions. The details of the protocol have been described in our previous research …”

Section: Methodsmentioning

confidence: 99%

“…Total RNA was extracted using TRIzol (Sigma Chemical Co. St. Louis, MO, USA) according to the manufacturer's instructions. Quantification was performed according to our previous study . The sequences of the primers (synthesized by Generay Biotech, Shanghai, China) used for PCR amplification are listed in Table .…”

Section: Methodsmentioning

confidence: 99%

“…Western blot analysis was performed as described in our previous study . The antibodies used were goat polyclonal anti‐CRHR1, goat polyclonal anti‐CRHR2, rabbit polyclonal anti‐CYP11A1, rabbit polyclonal anti‐CYP11B1 (all (1 : 1000 dilution), mouse monoclonal β‐actin (1 : 1000 dilution) and HRP‐conjugated secondary antibody (1 : 1000 dilution) (all Santa Cruz Biotechnology).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Icariin inhibits inflammation via immunomodulation of the cutaneous hypothalamus-pituitary-adrenal axis in vitro

et al. 2018

View full text Add to dashboard Cite

show abstract

Icariin inhibits TNF-α/IFN-γ induced inflammatory response via inhibition of the substance P and p38-MAPK signaling pathway in human keratinocytes

Cited by 90 publications

References 28 publications

Thymus vulgaris alleviates UVB irradiation induced skin damage via inhibition of MAPK/AP‐1 and activation of Nrf2‐ARE antioxidant system

Thymus vulgaris alleviates UVB irradiation induced skin damage via inhibition of MAPK/AP‐1 and activation of Nrf2‐ARE antioxidant system

Therapeutic Effects of Apamin as a Bee Venom Component for Non-Neoplastic Disease

Icariin inhibits inflammation via immunomodulation of the cutaneous hypothalamus-pituitary-adrenal axis in vitro

Contact Info

Product

Resources

About