Icariin Promote Stem Cells Regeneration and Repair Acinar Cells in L-arginine / Radiation -Inducing Chronic Pancreatitis in Rats

Abstract

Objective: Chronic Pancreatitis (CP) is a multifactorial disease. It was characterized by severe inflammation and acinar cell destruction. Thus, the present study was initiated to evaluating the ability of bone marrow-based mesenchymal stem cell (MSCs) combined with Icariin to restore and regenerate acinar cells in the pancreas of rats suffering chronic pancreatitis. Methods: Chronic pancreatitis was induced in rats via both L-arginine plus radiation, repeated L-arginine injection (2.5g/Kg body-weight, 1, 4,7,10,13,16,19 days), then, on day 21, rats were exposed to a single dose of gamma-radiation (6 Gy), which exacerbate injury of pancreatic acinar cells. One day after irradiation, rats were treated with either MSCs (1 × 107 /rat, once, tail vein injection) labeled PKH26 fluorescent linker dye and/or Icariin (100 mg/Kg, daily, orally) for 8 weeks. Results: Icariin promotes MSCs proliferation boosting its productivity in vitro. MSCs, and/or icariin treatments has regulated molecular factors TGF-β/PDGF and promoted the regeneration of pancreatic tissues by releasing PDX-1 and MafA involved in the recruitment of stem/progenitor cell in the tissue, and confirmed by histopathological examination. Moreover, a significant decrease in IL-8 and TNF-α cytokines with significant amelioration of myeloperoxidase activity were noted. As well as, reduction in MCP-1 and collagen type-1 levels along with Hedgehog signaling down-regulating expression in such cells, Patched-1, Smoothened, and GLi-1. Conclusion: The potent bioactive therapeutic Icariin combined with MSCs induces a significantly greater improvement, compared to each therapy alone.

Possible Therapeutic Effect of Ghrelin and exosomes derived from Mesenchymal stem cells on induced chronic pancreatitis of adult albino rats (Histological and Immunohistochemical Study)

“…Significance at P < 0.01 The mechanism of chronic pancreatitis induction by l-arginine was explained by (16,17) agreed with some investigators (16,18,19) and explained by others (17,20)…”

The neuroprotective effects of icariin on ageing, various neurological, neuropsychiatric disorders, and brain injury induced by radiation exposure

“…Icariin and icaritin have also been reported to inhibit UVB-induced photoaging by activating Nrf2/ARE signaling, suppressing JNK’s and ERK’s phosphorylation as well as NF-ĸB’s expression [ 138 ]. In a recent study, icariin was found to significantly increase bone marrow mesenchymal stem cells (MSCs) regeneration and repair pancreatic injury in L-arginine/γ-ray-caused rat model of chronic pancreatitis [ 139 ].…”

Curative role of mesenchymal stromal cells in chronic pancreatitis: Modulation of MAPK and TGF-β1/SMAD factors

“…Owing to no organ other than the pancreas appears to be impaired by L-arginine and it does not require surgery intervention. 10 A few studies have evaluated potential role of MSCs in chronic pancreatitis’, in which the persistence and consequences of the inflammatory response led to the formation of the extensive fiber region, as a change of pancreatic stellate cell (PSC) to myofibroblast shape. Establishing fibrotic region as a replacement for glandular parenchyma, thus minimizing pancreatic functionality.…”