Icariin promotes early and late stages of fracture healing in rats

Gürbüz, Kaan; Yerer, Mükerrem Betül; Gürbüz, Perihan; Halıcı, Mehmet

doi:10.5606/ehc.2019.66796

Cited by 10 publications

(9 citation statements)

References 13 publications

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“…Thus, it is essential to explore an alternative drug for BMP-2. Recently, a traditional Chinese medicine Icariin, the main therapeutic component of a traditional medicine Herba Epimedii, is reported to be cost-efficient, osteoinductive, and nontoxic [78][79][80][81]. This makes it an attractive alternative for BMP-2 in bone tissue engineering; however, the half-life of Icariin is too short to maintain a therapeutic concentration in bone defects.…”

Section: Icariinmentioning

confidence: 99%

Biomimetic Calcium Phosphate Coating as a Drug Delivery Vehicle for Bone Tissue Engineering: A Mini-Review

et al. 2020

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The high incidence of bone defect cases necessitates a rapid development of cost-effective bone tissue engineering approaches. Bone growth factors such as bone morphogenetic protein-2 (BMP-2) play a vital role in bone tissue engineering. The osteoinductive efficacy of BMP-2 and other osteogenic growth factors is highly dependent on their dose and delivery mode. Slow and sustained delivery of a low dose of BMP-2 promotes bone defect healing. However, the burst release of a high dose of BMP-2 not only fails to promote bone healing but also causes adverse local and systemic effects. The biomimetic calcium phosphate (BioCaP) coating technique guarantees a slow, gradual, and local release of BMP-2 to mimic its natural release from the bone matrix under physiological conditions. Therefore, BioCaP coatings have long been regarded as a promising drug carrier for growth factors. In this mini-review, we give a brief introduction to BioCaP coatings regarding their preparation techniques, physicochemical properties, potential as a drug carrier, ability to suppress foreign body reactivity, and their preclinical application in ectopic and orthotopic models. Finally, the current challenges and future prospects of BioCaP coatings are discussed.

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Section: Icariinmentioning

confidence: 99%

Biomimetic Calcium Phosphate Coating as a Drug Delivery Vehicle for Bone Tissue Engineering: A Mini-Review

et al. 2020

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“…The effects of several substances on fracture healing have been examined in the literature. An animal model study by Gürbüz et al[ 4 ] showed that pentoxifylline accelerated fracture healing in the early period and delayed histological union in the late period in rats. As a result of radiological findings, pentoxifylline use was found to be ineffective on fracture healing.…”

Section: Discussionmentioning

confidence: 99%

“…Some agents like locally administered icariin facilitates bone healing via improving antioxidant responses within the fracture site. [ 4 ] When the inflammatory response begins to diminish, necrotic tissue and exudate are reabsorbed, and fibroblasts and chondrocytes emerge and begin to produce the new matrix and fracture callus. [ 5 ] Fracture hematoma acts as a skeleton made of fibrin to facilitate the activity of repair cells.…”

Section: Introductionmentioning

confidence: 99%

Systemic tranexamic acid promotes bone healing in a rat model of femur fracture

et al. 2020

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Objectives This study aims to investigate the effect of tranexamic acid (TXA) on the union of closed femoral fractures using radiological and histological methods in an experimental rat model. Materials and methods This experimental study was conducted between June 2017 and February 2018. Closed femoral fractures were created in 36 male Wistar albino rats (age: three months [range, 2.5-3.5 months], weighing 200 grams [range, 180-220 grams]). Half of the animals randomly divided into two groups were administered intravenous single dose of TXA (30 mg/kg), whereas the animals in the control group did not receive any medication. The animals in the two groups were randomly divided into three groups with six animals each and cervical dislocation was performed at days 15, 30, and 45, and radiological and histopathological healing scores were compared. Results When the mean radiological scores of the TXA and control groups were compared, a statistically significant difference was found in favor of the TXA group at day 15 (p=0.019), but no significant difference was found in the mean scores on days 30 and 45 (p=0.138 and p=0.269, respectively). Histopathological examination also showed a statistically significant difference between the 15-day mean score values in favor of the TXA group ( p = 0. 017 ). Conclusion The use of systemic TXA accelerates early bone formation and fracture healing.

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“…[ 5 - 7 ] Therefore, researchers have proposed various alternatives in order to accelerate fracture healing, and eliminate delayed union and non-union. [ 31 ] One of these alternatives is the use of stem cells on bony tissue. [ 9 ] Taguchi et al[ 10 ] have evaluated in vivo migration of bone marrow cells and their contribution to bone repair and shown that MSCs were definitely associated with bone healing.…”

Section: Discussionmentioning

confidence: 99%

Can injection of adipose stem cells to non-union zone increase bone union? Experimental rat study

et al. 2020

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Fracture non-union is a chronic condition characterized by pain and functional disability. According to the diagnostic criteria established by the United States Food and Drug Administration, a non-union occurs when a fracture fails to union over a period of nine or more months or fails to prove any radiographic clues of healing response in an osseous environment within the previous three months of the follow-up. [1] Non-unions are seen in a small percentage of cases in which the biological process of fracture repair cannot overcome the local biology and mechanics of bony injury. This leads to a large number of procedures to treat non-healing fractures, increasing morbidity for patients and costs. Non-union of fractures have become an important health problem. Non-union still remains as a problem in some fractures despite the developments in surgical techniques and materials used in fracture repair. Delayed healing or non-union is an expected situation Objectives: This study aims to evaluate the effects of local adipose stem cell injection on non-union and diabetic non-union of rat femurs Materials and methods: Forty-eight female Wistar albino rats (weighing mean 200 g and aged 8 weeks) were used in this study. The rats were divided into six groups. Group 1 was chosen as a reference for receptor activator of nuclear factor-kappa (k) B (RANK), receptor activator of nuclear factor-k B ligand (RANKL) and osteoprotegerin (OPG) genes and no femur osteotomy was performed in this group. Group 2 underwent femur osteotomy, the osteotomy was fixed with a 1.5 mm K-wire as retrograde from the knee joint, and no gap was left in the osteotomy line. In order to induce non-union, femurs underwent osteotomy fixed with K-wires in groups 3, 4, 5 and 6. In addition, the osteotomy line was measured as 1.8 mm gap with electronic calipers and the gap was fixed with U staple. Before osteotomy, streptozocin was injected intraperitoneally at a dose of 60 mg/kg in 0.1 mol/L citrate buffer solution (Ph 4.4) in groups 4 and 6, in order to induce diabetes mellitus. Left femur anteroposterior and lateral X-rays were taken 10 weeks after the operation and the union in group 2 and non-union in groups 3, 4, 5, and 6 were confirmed. To see if injection of adipose stem cells into the non-union site increases bone union, 2 mL 0.9% sodium chloride (NaCl) in groups 3 and 4 and 2×10 6 adipose stem cell in groups 5 and 6 were locally injected into the non-union area with fluoroscopy. Femur X-rays were taken eight weeks after the injection and all rats were sacrificed. Femurs of rats were removed for histopathological and gene expression evaluation. Results: There were significant differences between the groups injected 0.9% NaCI and adipose stem cells in terms of bone healing according to radiological and histopathological evaluations (p<0.05). No statistically significant difference was observed between the groups in terms of gene expression levels. Conclusion: According to the results of our study, local adipose stem cell injection has positive radiolo...

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Icariin promotes early and late stages of fracture healing in rats

Cited by 10 publications

References 13 publications

Biomimetic Calcium Phosphate Coating as a Drug Delivery Vehicle for Bone Tissue Engineering: A Mini-Review

Biomimetic Calcium Phosphate Coating as a Drug Delivery Vehicle for Bone Tissue Engineering: A Mini-Review

Systemic tranexamic acid promotes bone healing in a rat model of femur fracture

Can injection of adipose stem cells to non-union zone increase bone union? Experimental rat study

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