ICE
 Sp2905
 , the
 erm
 (TR)-
 tet
 (O) Element of Streptococcus pyogenes, Is Formed by Two Independent Integrative and Conjugative Elements

Giovanetti, Eleonora; Brenciani, Andrea; Tiberi, Erika; Bacciaglia, Alessandro; Varaldo, Pietro E.

doi:10.1128/aac.05352-11

Cited by 35 publications

(31 citation statements)

References 21 publications

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“…The origin of replication of the chromosome (Ori) is also shown. All elements matched with homologous elements found in the S. agalactiae sequenced genomes except for the ICE inserted in the rumA gene, which belongs to the ICE Sp2905 -like family of elements found in S. pyogenes [42]. ( b ) Genetic map of the IME integrated 3′ to the rpsI gene (IMESag- rpsI ; 9496 bp).…”

Section: Resultsmentioning

confidence: 94%

Streptococcal group B integrative and mobilizable element IMESag-rpsIencodes a functional relaxase involved in its transfer

et al. 2016

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Streptococcus agalactiae or Group B Streptococcus (GBS) are opportunistic bacteria that can cause lethal sepsis in children and immuno-compromised patients. Their genome is a reservoir of mobile genetic elements that can be horizontally transferred. Among them, integrative and conjugative elements (ICEs) and the smaller integrative and mobilizable elements (IMEs) primarily reside in the bacterial chromosome, yet have the ability to be transferred between cells by conjugation. ICEs and IMEs are therefore a source of genetic variability that participates in the spread of antibiotic resistance. Although IMEs seem to be the most prevalent class of elements transferable by conjugation, they are poorly known. Here, we have studied a GBS-IME, termed IMESag-rpsI, which is widely distributed in GBS despite not carrying any apparent virulence trait. Analyses of 240 whole genomes showed that IMESag-rpsI is present in approximately 47% of the genomes, has a roughly constant size (approx. 9 kb) and is always integrated at a single location, the 3′-end of the gene encoding the ribosomal protein S9 (rpsI). Based on their genetic variation, several IMESag-rpsI types were defined (A–J) and classified in clonal complexes (CCs). CC1 was the most populated by IMESag-rpsI (more than 95%), mostly of type-A (71%). One CC1 strain (S. agalactiae HRC) was deep-sequenced to understand the rationale underlying type-A IMESag-rpsI enrichment in GBS. Thirteen open reading frames were identified, one of them encoding a protein (MobSag) belonging to the broadly distributed family of relaxases MOBV1. Protein MobSag was purified and, by a newly developed method, shown to cleave DNA at a specific dinucleotide. The S. agalactiae HRC-IMESag-rpsI is able to excise from the chromosome, as shown by the presence of circular intermediates, and it harbours a fully functional mobilization module. Further, the mobSag gene encoded by this mobile element is able to promote plasmid transfer among pneumococcal strains, suggesting that MobSag facilitates the spread of IMESag-rpsI and that this spread would explain the presence of the same IMESag-rpsI type in GBS strains belonging to different CCs.

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Section: Resultsmentioning

confidence: 94%

Streptococcal group B integrative and mobilizable element IMESag-rpsIencodes a functional relaxase involved in its transfer

et al. 2016

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“…The early study of CW459tet(M) (7) and later studies of genetic elements related to Tn5801 (10,18,19) concur in describing an integration site just downstream of guaA, a chromosomal gene encoding a GMP synthase that is consistently found adjacent to int 5801 in the sequenced genomes containing a Tn5801 element. Using strategies refined in previous studies (16,(20)(21)(22), this site was thus explored in the 19 S. agalactiae isolates harboring Tn5801.Sag. The genome of S. agalactiae strain 2603V/R (GenBank accession no.…”

Section: Characterization Of Tet-resistantmentioning

confidence: 99%

Characterization of Tn 5801.Sag , a Variant of Staphylococcus aureus Tn 916 Family Transposon Tn 5801 That Is Widespread in Clinical Isolates of Streptococcus agalactiae

Mingoia

Morici

Tili

et al. 2013

Antimicrob Agents Chemother

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Tn5801, originally detected in Staphylococcus aureus Mu50, is a Tn916 family element in which a unique int gene (int 5801 ) replaces the int and xis genes in Tn916 (int 916 and xis 916 ). Among 62 tet(M)-positive tetracycline-resistant Streptococcus agalactiae isolates, 43 harbored Tn916, whereas 19 harbored a Tn5801-like element (Tn5801.Sag, ϳ20.6 kb). Tn5801.Sag was characterized (PCR mapping, partial sequencing, and chromosomal integration) and compared to other Tn5801-like elements. Similar to Tn5801 from S. aureus Mu50, tested in parallel, Tn5801.Sag was unable to undergo circularization and conjugal transfer.

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“…The transposon further confers resistance to streptomycin via the aadE gene [29]. ICESp2905 is a widespread erm(A)-and tet(O)-carrying genetic element of S. pyogenes, resulting from one ICE (ICESp2907) being integrated into another ICE (ICESp2906) of clostridial origin [44]. Originally, the MLS resistance determinant was named erm(TR), but it has been renamed to erm(A).…”

Section: Tetracycline and Mls Resistance Genes And Transposonsmentioning

confidence: 99%

Resistance to the tetracyclines and macrolide-lincosamide-streptogramin group of antibiotics and its genetic linkage – a review

Marosevic¹,

Kaevska²,

Jaglič³

2017

Ann Agric Environ Med.

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Marosevic D, Kaevska M, Jaglic Z. Resistance to the tetracyclines and macrolide-lincosamide-streptogramin group of antibiotics and its genetic linkage -a review. Ann Agric Environ Med. 2017; 24(2): 338-344. https://doi.org/10.26444/aaem/74718 AbstractAn excessive use of antimicrobial agents poses a risk for the selection of resistant bacteria. Of particular interest are antibiotics that have large consumption rates in both veterinary and human medicine, such as the tetracyclines and macrolidelincosamide-streptogramin (MLS) group of antibiotics. A high load of these agents increases the risk of transmission of resistant bacteria and/or resistance determinants to humans, leading to a subsequent therapeutic failure. An increasing incidence of bacteria resistant to both tetracyclines and MLS antibiotics has been recently observed. This review summarizes the current knowledge on different tetracycline and MLS resistance genes that can be linked together on transposable elements.

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ICE Sp2905 , the erm (TR)- tet (O) Element of Streptococcus pyogenes, Is Formed by Two Independent Integrative and Conjugative Elements

Cited by 35 publications

References 21 publications

Streptococcal group B integrative and mobilizable element IMESag-rpsIencodes a functional relaxase involved in its transfer

Streptococcal group B integrative and mobilizable element IMESag-rpsIencodes a functional relaxase involved in its transfer

Characterization of Tn 5801.Sag , a Variant of Staphylococcus aureus Tn 916 Family Transposon Tn 5801 That Is Widespread in Clinical Isolates of Streptococcus agalactiae

Resistance to the tetracyclines and macrolide-lincosamide-streptogramin group of antibiotics and its genetic linkage – a review

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