Abstract. Since 2003, the state-of-the-art Canadian Coast Guard Ship (CCGS) research icebreaker Amundsen furrows the Canadian Arctic waters to support novel research endeavors and collect oceanographic data. This paper presents the data acquisition, the processing methods and an overview of the data collected during the 2021 expedition as the ship traveled over 30 000 km during 122 days across the Canadian Arctic Ocean, collecting sea surface, atmospheric and seabed underway measurements. A total of 266 casts of a conductivity, temperature and depth profiler mounted on a rosette (CTD-Rosette) were also conducted to monitor the main physical, chemical and biological parameters of the water column. More specifically, the data here presented were collected with the CTD-Rosette across historical sampling transects in Davis Strait, the NorthWater Polynya (NOW), and Cape Bathurst. A 182 km dedicated survey of Moving Vessel Profiler (MVP), equipped with CTD, transmissometer, dissolved oxygen, fluorescence, sound velocity sensors, was conducted across Hudson Strait. We also present an overview of the data collected by the underway systems (seabed, thermosalinograph and atmospheric). Such data are essential in understanding the impacts of climate warming on the unique environments of the Canadian Arctic Ocean. Amundsen Science supports and promotes easy access and sharing of such valuable data to the scientific community.