ICP-MS-Based Methodology In Metallomics: Towards Single Particle Analysis, Single Cell Analysis, And Spatial Metallomics

Chen, Mingli; Wang, Meng

doi:10.46770/as.2022.108

Cited by 4 publications

(3 citation statements)

References 94 publications

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“…After ion beam concentration via the ion optics, the ionized isotopes were detected via the mass spectrometer (MS). ICP-MS has excellent detection limits for most elements in the periodic table (sub-femtogram levels), a wide linear dynamic range (8 orders of magnitude), high analytical throughput, and minimal matrix effects 22 . ICP-MS has now evolved into a versatile platform technology with hyphenated techniques, and exhibits great compatibility with varied sample forms such as solution, tissue slices and even a single cell 23 , 24 .…”

Section: Resultsmentioning

confidence: 99%

Selenium-based metabolic oligosaccharide engineering strategy for quantitative glycan detection

Tian,

Zheng,

Wang

et al. 2023

Nat Commun

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Metabolic oligosaccharide engineering (MOE) is a classical chemical approach to perturb, profile and perceive glycans in physiological systems, but probes upon bioorthogonal reaction require accessibility and the background signal readout makes it challenging to achieve glycan quantification. Here we develop SeMOE, a selenium-based metabolic oligosaccharide engineering strategy that concisely combines elemental analysis and MOE,enabling the mass spectrometric imaging of glycome. We also demonstrate that the new-to-nature SeMOE probes allow for detection, quantitative measurement and visualization of glycans in diverse biological contexts. We also show that chemical reporters on conventional MOE can be integrated into a bifunctional SeMOE probe to provide multimodality signal readouts. SeMOE thus provides a convenient and simplified method to explore the glyco-world.

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Section: Resultsmentioning

confidence: 99%

Selenium-based metabolic oligosaccharide engineering strategy for quantitative glycan detection

Tian,

Zheng,

Wang

et al. 2023

Nat Commun

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“…Traditional measurements of elemental abundance, on a bulk scale, tend to neglect the intricacies and disruptions of metal homeostasis within specific tissue architectures, obscuring critical associations and insights (Moses and Pachter, 2022). Spatially resolved metal analysis through techniques like laser ablation inductively coupled plasma time-of-flight mass spectrometry (LA-ICPTOF-MS) offers detailed maps of multi-elemental distributions at one-micron resolution (Bussweiler et al ., 2017; Theiner et al ., 2019; Löhr et al ., 2019; da Silva and Arruda, 2022; Clases and Gonzalez de Vega, 2022a, 2022b; Chang et al ., 2022). Metal ions are essential for the functioning of many biomolecules, including proteins.…”

Section: Methodsmentioning

confidence: 99%

Integrative Co-Registration of Elemental Imaging and Histopathology for Enhanced Spatial Multimodal Analysis of Tissue Sections through TRACE

Lu,

Steiner,

Han

et al. 2024

Preprint

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Summary: Elemental imaging provides detailed profiling of metal bioaccumulation, offering more precision than bulk analysis by targeting specific tissue areas. However, accurately identifying comparable tissue regions from elemental maps is challenging, requiring the integration of hematoxylin and eosin (H&E) slides for effective comparison. Facilitating the streamlined coregistration of Whole Slide Images (WSI) and elemental maps, TRACE enhances the analysis of tissue regions and elemental abundance in various pathological conditions. Through an interactive containerized web application, TRACE features real time annotation editing, advanced statistical tools, and data export, supporting comprehensive spatial analysis. Notably, it allows for comparison of elemental abundances across annotated tissue structures and enables integration with other spatial data types through WSI coregistration. Availability and Implementation: Available on the following platforms- GitHub: jlevy44/trace_app, PyPI: trace_app, Docker: joshualevy44/trace_app, Singularity: joshualevy44/trace_app.

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“…To examine elemental composition of single particle by detection of multiple isotopes, ICP-TOF-MS can be used, providing isotopic masses in particles [28,88]. The elements consisting of single particle can be distinguished based on the differences in the time required to reach the detector [4].…”

Section: Determination Of the Elemental And Isotopic Composition Of Npsmentioning

confidence: 99%

Basic and advanced spectrometric methods for complete nanoparticles characterization in bio/eco systems: current status and future prospects

Borowska

Jankowski

2023

Anal Bioanal Chem

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The use of engineered nanoparticles in the environment and human life has increased in the last 20 years. The risk assessment concerning application of nanomaterials in biological systems requires their thorough characterization. Understanding the correlations between physicochemical properties of nanoparticles concerning not only the size, particle size distribution, number concentration, degree of aggregation, or agglomeration but also solubility, stability, binding affinity, surface activity, chemical composition, and nanoparticle synthesis yield allows their reliable characterization. Thus, to find the structure-function/property relationship of nanoparticles, multifaceted characterization approach based on more than one analytical technique is required. On the other hand, the increasing demand for identification and characterization of nanomaterials has contributed to the continuous development of spectrometric techniques which enables for their qualitative and quantitative analysis in complex matrices giving reproducible and reliable results. This review is aimed at providing a discussion concerning four main aspects of nanoparticle characterization: nanoparticle synthesis yield, particle size and number concentration, elemental and isotopic composition of nanoparticles, and their surface properties. The conventional and non-conventional spectrometric techniques such as spectrophotometry UV-Vis, mass spectrometric techniques working in conventional and single-particle mode, or those based on optical emission detection systems are described with special emphasis paid on their advantages and drawbacks. The application and recent advances of these methods are also comprehensively reviewed and critically discussed. Graphical abstract

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ICP-MS-Based Methodology In Metallomics: Towards Single Particle Analysis, Single Cell Analysis, And Spatial Metallomics

Cited by 4 publications

References 94 publications

Selenium-based metabolic oligosaccharide engineering strategy for quantitative glycan detection

Selenium-based metabolic oligosaccharide engineering strategy for quantitative glycan detection

Integrative Co-Registration of Elemental Imaging and Histopathology for Enhanced Spatial Multimodal Analysis of Tissue Sections through TRACE

Basic and advanced spectrometric methods for complete nanoparticles characterization in bio/eco systems: current status and future prospects

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