ICSI and severe male-factor infertility: breaking the sperm tail prior to injection

Gerris, Jan; Mangelschots, K; Royen, E Van; Joostens, M.; Eestermans, W.; Ryckaert, G

doi:10.1093/oxfordjournals.humrep.a135968

Cited by 48 publications

(22 citation statements)

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“…It is well established that sperm immobilization immediately before intracytoplasmic sperm injection (ICSI) increases the rate of successful fertilization [1][2][3][4][5][6]. The reason for this is not clear, but Yanagimachi [7] inferred that disruption or labilization of the sperm plasma membrane facilitates its disintegration and thereby directs contact of sperm components with the ooplasm.…”

Section: Introductionmentioning

confidence: 99%

Sonication Per Se Is Not as Deleterious to Sperm Chromosomes as Previously Inferred1

Tateno

Kimura

Yanagimachi

2000

Biology of Reproduction

109

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Although sonication is a simple way to immobilize ("kill") spermatozoa prior to injection into oocytes, this has been thought to be destructive to sperm chromosomes. Mouse and human spermatozoa were immobilized by sonication and kept in various media for up to 2 h, then their nuclei were individually injected into mouse oocytes for the analysis of chromosomes at the first cleavage metaphase. In both the mouse and human, incidence of structural chromosome aberrations was much higher in the spermatozoa sonicated and stored in Biggers-Whitten-Whittingham medium for 2 h at 37.5 degrees C than in those stored for 5 min in the same medium. We concluded, therefore, that it is not sonication per se but a prolonged exposure of sperm nuclei to extracellular milieu that is detrimental to sperm chromosomes. The incidence of structural chromosome aberrations of mouse and human spermatozoa was significantly reduced when the spermatozoa were sonicated and stored in K(+)-rich nucleus isolation medium containing EDTA. This suggests that sperm chromosome degradation following sperm immobilization by sonication is partly due to detrimental effects of a Na(+)-rich medium and of DNase on sperm chromatin. Ideally, it should be possible to prepare artificial media that maintain the integrity of sperm chromosomes for many hours after immobilization.

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Section: Introductionmentioning

confidence: 99%

Sonication Per Se Is Not as Deleterious to Sperm Chromosomes as Previously Inferred1

Tateno

Kimura

Yanagimachi

2000

Biology of Reproduction

109

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“…These conditions, although not always necessary, allowed for the highest frequency of normal fertilization. It has been previously shown that partial modifications of the human sperm plasma membrane are necessary to allow decondensation of the sperm nucleus after it has entered the oocyte [20][21][22]. Breaking of the tail of the spermatozoon may contribute to such membrane damage and evoke physical or biochemical changes similar to those occurring during natural gamete fusion [21,23].…”

Section: Discussionmentioning

confidence: 99%

Microtubule and Chromatin Configurations during Rhesus Intracytoplasmic Sperm Injection: Successes and Failures1

Hewitson

Simerly

Tengowski

et al. 1996

Biology of Reproduction

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Intracytoplasmic sperm injection (ICSI) was performed on rhesus monkey oocytes, and the resultant microtubule and DNA configurations were imaged by laser-scanning confocal microscopy. In addition, polyspermic oocytes fertilized by ICSI were examined by transmission electron microscopy (TEM). Successful rhesus fertilization by ICSI revealed microtubule and DNA configurations similar to those observed during in vitro fertilization of human and rhesus monkey oocytes, including sperm aster formation, pronuclei decondensation, spindle formation, and cell division. Several abnormalities, however, were also observed: 1) inability to complete meiosis; 2) inability to undergo male or female pronucleus formation; 3) separation of the sperm tail from the sperm nucleus; 4) premature chromosome condensation with the formation of a paternal meiotic spindle; and 5) formation of multiple female pronuclei (karyomeres) during chromosome decondensation. TEM analysis revealed that sperm can undergo decondensation in the presence of an intact acrosome at least 18 h after sperm injection. These results demonstrate the utility of rhesus ICSI in pre-clinical applications as well as with endangered species. However, the different types of fertilization failures observed here indicate that although ICSI may be a readily accepted means of fertilization of human oocytes in many clinics, we should further characterize the cellular and genetic abnormalities associated with ICSI in both human and nonhuman primates.

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“…Similarly, the expression of transcripts for antioxidant agents was found during embryo culture on bovine oviductal cell monolayers [20]. As [13,44], was sufficient enough in equine species to allow fertilization. Dozortev et al [12] and Van den Bergh et al [44] …”

Section: Introductionmentioning

confidence: 85%

Preliminary observations in in vitro development of equine embryo after ICSI

Guignot¹,

Ottogalli²,

Yvon³

et al. 1998

Reprod. Nutr. Dev.

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ICSI and severe male-factor infertility: breaking the sperm tail prior to injection

Cited by 48 publications

References 0 publications

Sonication Per Se Is Not as Deleterious to Sperm Chromosomes as Previously Inferred1

Sonication Per Se Is Not as Deleterious to Sperm Chromosomes as Previously Inferred1

Microtubule and Chromatin Configurations during Rhesus Intracytoplasmic Sperm Injection: Successes and Failures1

Preliminary observations in in vitro development of equine embryo after ICSI

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