ICT-modulated NIR water-soluble fluorescent probe with large Stokes shift for selective detection of cysteine in living cells and zebrafish

Recent research progress of red-emitting/near-infrared fluorescent probes for biothiols

“…In 2020, Xu's team reported an ICT-based near-infrared water-soluble fluorescent probe 9 for the detection of Cys. 40 The probe was obtained by introducing the acrylate into the fluorophore composed of 1,8-naphthalimide and tricyanofuran, which prevented the ICT process between 1,8-naphthalimide and tricyanofuran, causing probe 9 with weak fluorescence. After reacting with Cys in PBS solution, the effect of ICT was restored and the fluorescence of probe 9 at 665 nm was enhanced by about 40 times (Fig.…”

Near-Infrared Probes for Biothiols (Cysteine, Homocysteine, and Glutathione): A Comprehensive Review

“…The in vitro and in vivo studies confirmed the good impact of NIR-50 in biological applications. Xu et al 131 reported 1,8-naphthalimide and tricyanofuranbased water-soluble probe NIR-51 for Cys. Initially, the low fluorescence of NIR-51 was enhanced in the presence of Cys at the emission maximum of 665 nm (λ ex = 620 nm).…”

A wash-free fluorescent probe with a large Stokes shift for the identification of NAFL through tracing the change of lipid droplets

“…Consequently, D–π–A type ICT probes possess advantageous characteristics such as long wavelength emission and large Stokes shifts, making them highly desirable in biological imaging. 18–20…”