ICTV Virus Taxonomy Profile: Baculoviridae

Harrison, Robert L.; Herniou, Elisabeth A.; Jehle, Johannes A.; Theilmann, David A.; Burand, John P.; Becnel, James J.; Krell, Peter J.; Oers, Monique M. van; Mowery, Joseph; Bauchan, Gary R.

doi:10.1099/jgv.0.001107

Cited by 125 publications

(101 citation statements)

References 5 publications

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“…A previously unannotated hypothetical ORF located between ORF54 and lef-9 was identified but not annotated in all published HaSNPV genomes. Clustering of H. zea and H. armigera isolates based on MLEs from whole-genome and ORF sequences supported the classification of the viruses as a single species [12]. …”

Section: Discussionmentioning

confidence: 96%

“…The genetic diversity of the Group II nucleopolyhedroviruses (genus Alphabaculoviruses ) from Lepidoptera of the genus Helicoverpa are of importance due to their worldwide distribution and widespread use as biopesticides against these significant polyphagous pests [3]. Group II singly-enveloped nucleopolyhedroviruses from species of the genus Helicoverpa (Lepidoptera: Noctuidae) were originally classified into two species; Old World H. armigera single nucleopolyhedrovirus (HaSNPV), isolated from H. armigera (Hübner) and New World H. zea single nucleopolyhedrovirus (HzSNPV) isolated from H. zea (Boddie) [3,4,5,6,7,8,9,10,11,12,13]. This has been recently revised to classify both types as a single species, HaSNPV, with similarities in DNA sequence and biological activity [12].…”

Section: Introductionmentioning

confidence: 99%

“…and of nine strains derived by passage in tissue culture and insects. We compare whole genome sequences and sequences of selected hypothetical and functional ORFs to determine patterns of strain selection and evolution [12,17] in comparison to sequences from both Old and New World isolates. Throughout, we use HaSNPV to refer to the Helicoverpa SNPV virus species but identify isolates from the insect H. zea as HzSNPV to differentiate isolates from that of the host and where sequences use the old nomenclature.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Analysis of HaSNPV-AC53 and Derived Strains

Noune

Hauxwell

2016

Viruses

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Complete genome sequences of two Australian isolates of H. armigera single nucleopolyhedrovirus (HaSNPV) and nine strains isolated by plaque selection in tissue culture identified multiple polymorphisms in tissue culture-derived strains compared to the consensus sequence of the parent isolate. Nine open reading frames (ORFs) in all tissue culture-derived strains contained changes in nucleotide sequences that resulted in changes in predicted amino acid sequence compared to the parent isolate. Of these, changes in predicted amino acid sequence of six ORFs were identical in all nine derived strains. Comparison of sequences and maximum likelihood estimation (MLE) of specific ORFs and whole genome sequences were used to compare the isolates and derived strains to published sequence data from other HaSNPV isolates. The Australian isolates and derived strains had greater sequence similarity to New World SNPV isolates from H. zea than to Old World isolates from H. armigera, but with characteristics associated with both. Three distinct geographic clusters within HaSNPV genome sequences were identified: Australia/Americas, Europe/Africa/India, and China. Comparison of sequences and fragmentation of ORFs suggest that geographic movement and passage in vitro result in distinct patterns of baculovirus strain selection and evolution.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Analysis of HaSNPV-AC53 and Derived Strains

Noune

Hauxwell

2016

Viruses

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“…The family Baculoviridae comprises a diverse group of large double stranded DNA viruses that infect larvae of the insect orders Lepidoptera, Hymenoptera and Diptera [1]. Baculovirus have a rod shaped, enveloped virion with a circular genome ranging from 80 to 180 kbp [2].…”

Section: Introductionmentioning

confidence: 99%

“…These infected cells produce budded viruses (BVs) that disseminate the infection systemically [3]. Based on OBs morphology, baculoviruses were first classified in two groups: Nucleopolyhedrovirus (NPV) and Granulovirus (GV) [1]. Later, they were taxonomically divided into four genera:…”

Section: Introductionmentioning

confidence: 99%

Protein composition of the occlusion bodies of Epinotia aporema granulovirus

Masson

Fabre

Ferrelli

et al. 2018

Preprint

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Within family Baculoviridae, members of the Betabaculovirus genus are employed as biocontrol agents against lepidopteran pests, either alone or in combination with selected members of the Alphabaculovirus genus. Epinotia aporema granulovirus (EpapGV) is a fast killing betabaculovirus that infects the bean shoot borer (E. aporema) and is a promising biopesticide. Because occlusion bodies (OBs) play a key role in baculovirus horizontal transmission, we investigated the composition of EpapGV OBs. Using mass spectrometry-based proteomics we could identify 56 proteins that are included in the OBs during the final stages of larval infection. Our data provides experimental validation of several annotated hypothetical coding sequences. Proteogenomic mapping against genomic sequence detected a previously unannotated ac110-like core gene and a putative translation fusion product of ORFs epap48 and epap49. Comparative studies of the proteomes available for the family Baculoviridae highlight the conservation of core gene products as parts of the occluded virion. Two proteins specific for betabaculoviruses (Epap48 and Epap95) are incorporated into OBs.Moreover, quantification based on emPAI values showed that Epap95 is one of the most abundant components of EpapGV OBs.

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Characterisation of extracellular vesicles in baculovirus infection of Spodoptera frugiperda cells

Van Es,

Possee,

King

2024

J of Extracellular Bio

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Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is an enveloped DNA virus of the Baculoviridae family. This baculovirus is widely exploited for the biological control of insect pest species and as an expression platform to produce recombinant proteins in insect cells. Extracellular vesicles (EVs) are secreted by all cells and are involved in key roles in many biological processes through their cargo consisting of proteins, RNA or DNA. In viral infections, EVs have been found to transfer both viral and cellular cargo that can elicit either a pro‐ or antiviral response in recipient cells. Here, small EVs (sEVs) released by Spodoptera frugiperda (Sf) insect cells were characterised for the first time. Using S. frugiperda (SfC1B5) cells stably expressing the baculovirus gp64, the viral envelope protein GP64 was shown to be incorporated into sEVs. Sf9 cells were also transfected with a bacmid AcMNPV genome lacking p6.9 (AcΔP6.9) to prevent budded virus production. The protein content of sEVs from both mock‐ and AcΔP6.9‐transfected cells were analysed by mass spectrometry. In addition to GP64, viral proteins Ac‐F, ME‐53 and viral ubiquitin were identified, as well as many host proteins including TSG101—which may be useful as a protein marker for sEVs.

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ICTV Virus Taxonomy Profile: Baculoviridae

Cited by 125 publications

References 5 publications

Comparative Analysis of HaSNPV-AC53 and Derived Strains

Comparative Analysis of HaSNPV-AC53 and Derived Strains

Protein composition of the occlusion bodies of Epinotia aporema granulovirus

Characterisation of extracellular vesicles in baculovirus infection of Spodoptera frugiperda cells

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