ICTV Virus Taxonomy Profile: Iflaviridae

Valles, Steven M.; Chen, Y; Firth, Andrew E.; Guérin, Diego M. A.; Hashimoto, Yoshifumi; Herrero, Salvador; Miranda, Joachim Rodrigues De; Ryabov, Eugene V.

doi:10.1099/jgv.0.000757

Cited by 137 publications

(125 citation statements)

References 4 publications

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“…The synergistic interaction between V. destructor and DWV is one of the major threats to the honey bee industry (Di Prisco et al 2016). DWV belongs to the Iflaviridae family (Valles et al 2017) so it is also a +ssRNA virus, like the new viruses described here. Therefore, it is possible that its replication and transmission of DWV in the mites could be influenced by the presence of other viruses from the same family (VdIV-2) or with similar replication mechanism (VdV-3 and VdV-5).…”

Section: Interaction Of the New Viruses With Dwvmentioning

confidence: 80%

“…The genome of the VdIV-2 UK contains a single large ORF, that translates into 2996 amino acids polyprotein, displaying the characteristic Iflaviridae structure organisation (Valles et al 2017) (Fig 1B). Conserved motifs for the Non-structural proteins, RdRp, 3C-like cysteine protease and RNA helicase, were detected in the C-terminal region of the polyprotein.…”

Section: New Viruses Associated To V Destructormentioning

confidence: 99%

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Identification of new viruses specific to the honey bee mite Varroa destructor

Herrero

Coll

et al. 2019

Preprint

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Large-scale colony losses among managed Western honey bees have become a serious threat to the beekeeping industry in the last decade. There are multiple factors contributing to these losses but the impact of Varroa destructor parasitism is by far the most important, along with the contribution of some pathogenic viruses vectored by the mite. So far, more than 20 viruses have been identified infecting the honey bee, most of them RNA viruses. They may be maintained either as covert infections or causing severe symptomatic infections, compromising the viability of the colony. In silico analysis of available transcriptomic data obtained from mites collected in the USA and Europe as well as additional investigation with new samples collected locally allowed the description of three novel RNA viruses. Our results showed that these viruses were widespread among samples and that they were present in the mites and in the bees but with differences in the relative abundance and prevalence. However, we have obtained strong evidence showing that these three viruses were able to replicate in the mite, but not in the bee, suggesting that they are selectively infecting the mite. To our knowledge, this is the first demonstration of Varroa-specific viruses, which open the door to future applications that might help controlling the mite through biological control approaches.

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Section: Interaction Of the New Viruses With Dwvmentioning

confidence: 80%

Section: New Viruses Associated To V Destructormentioning

confidence: 99%

Identification of new viruses specific to the honey bee mite Varroa destructor

Herrero

Coll

et al. 2019

Preprint

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“…DWV is the major virus pathogen of honey bees and is associated with increased colony losses worldwide that have affected pollination services and food security [23,24]. DWV belongs to the family Iflaviridae (order Picornavirales) which includes 15 recognized and 18 pending virus species infecting a wide range of insects [21]. It is very likely that the real diversity and distribution of this virus group is much wider and therefore it is likely to have a great impact on many invertebrate species.…”

Section: Discussionmentioning

confidence: 99%

Development of a honey bee RNA virus vector based on the genome of Deformed wing virus

Ryabov

Christmon

Heerman

et al. 2020

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We developed a honey bee RNA-virus vector based on the genome of a picorna-like Deformed wing virus (DWV), the main viral pathogen of the honey bee (Apis mellifera). To test the potential of DWV to be utilized as a vector, the 717 nt sequence coding for the enhanced green fluorescent protein (eGFP), flanked by the peptides targeted by viral protease, was inserted into an infectious cDNA clone of DWV in-frame between the leader protein and the virus structural protein VP2 genes. The in vitro RNA transcripts from egfp-tagged DWV cDNA clones were infectious when injected into honey bee pupae. Stable DWV particles containing genomic RNA of the recovered DWV with egfp inserts were produced, as evidenced by cesium chloride density gradient centrifugation. These particles were infectious to honey bee pupae when injected intra-abdominally. Fluorescent microscopy showed GFP expression in the infected cells and Western blot analysis demonstrated accumulation of free eGFP rather than its fusions with DWV LP and/or VP2 proteins. Analysis of the progeny egfp-tagged DWV showed gradual accumulation of genome deletions for egfp, providing estimates for the rate of loss of a non-essential gene an insect RNA virus genome during natural infection.

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“…Members of three positive-sense single-stranded RNA virus families such as Dicistroviridae (Bonning and Miller, 2010), Iflaviridae (Valles et al, 2017) and Picornaviridae (Zell, 2018) are widely reported from insects. These families were included in a new virus order Picornavirales (Le Gall et al, 2008).…”

mentioning

confidence: 99%

“…Several members of these insect small RNA virus families cause clear signs of disease in their hosts, such as infectious flacherie virus in silkworms (Valles et al, 2017), and deformed wing virus (Wilfert et al, 2016) and slow bee paralysis virus in honeybees (Bonning and Miller, 2010). It has been suggested that the picorna-like viruses or other members of Picornavirales can be maintained in insects by horizontal and vertical transmission .…”

mentioning

confidence: 99%

Identification of a Novel Picorna-like Virus in Coconut Rhinoceros Beetles (Oryctes rhinoceros)

Etebari

Shelomi

Furlong

2020

Preprint

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A novel Picorna-like virus, tentatively named Oryctes rhinoceros Picorna-like virus 1 (OrPV1), was identified in coconut rhinoceros beetle (Oryctes rhinoceros) larvae in Taiwan. The complete genome sequence consisted of 9,665 nucleotides with a polyA tail and included one open reading frame. Conserved structural domains such as Picornavirus capsid protein, RNA helicase, Peptidase and RNA-dependent RNA polymerase (RdRp) were identified through Pfam domain searches. The genome shares approximately 28% identity with other unclassified picornavirals that infect honey bees (Darwin bee virus 2, Bundaberg bee virus 5, and Sacbrood virus) and a recently reported virus from Asian lady beetle (Harmonia axyridis virus 1). We did not detect this virus in any other geographical populations of O. rhinoceros collected from the South Pacific Islands and the Philippines. Analysis of the deduced RdRp amino acid sequences showed that the virus clustered with other Picorna-like viruses and separated from other members of family Dicistroviridae and Iflaviridae.

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ICTV Virus Taxonomy Profile: Iflaviridae

Cited by 137 publications

References 4 publications

Identification of new viruses specific to the honey bee mite Varroa destructor

Identification of new viruses specific to the honey bee mite Varroa destructor

Development of a honey bee RNA virus vector based on the genome of Deformed wing virus

Identification of a Novel Picorna-like Virus in Coconut Rhinoceros Beetles (Oryctes rhinoceros)

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