Id1, Id2, and Id3 gene expression in neural cells during development

Tzeng, Shun Fen; Vellis, Jean de

doi:10.1002/(sici)1098-1136(199812)24:4<372::aid-glia2>3.0.co;2-b

Cited by 72 publications

(39 citation statements)

References 28 publications

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“…Furthermore, we demonstrated that there was a significant increase in Id4 expression after neu-creased signaling (63). These findings show that Shh is a niche factor regulating the number of stem cells and ronal differentiation, which was associated with more mature phenotypes of neural cells (69). they contribute to understand the mechanisms that modulate proliferation of stem cells.…”

Section: Changes In Hascs Gene Expressionmentioning

confidence: 58%

Differential Expression of Shh and BMP Signaling in the Potential Conversion of Human Adipose Tissue Stem Cells Into Neuron-Like Cells In Vitro

Cardozo

Ielpi²,

Gómez³

et al. 2010

gene expr

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The nervous system (NS) has a limited self-repair capability and adult neurogenesis is limited to certain regions of the brain. This generates a great interest in using stem cells to repair the NS. Previous reports have shown the differentiation of adipose tissue-derived mesenchymal stem cells (ASCs) in neuron-like cells when cultures are enriched with growth factors participating in embryonic and adult neurogenesis. Therefore, it could be thought that there exists a functional parallelism between neurogenesis and neuronal differentiation of ASCs. For this reason, the goal of this work was to study the differential gene expression of Shh and BMP genetic pathways involved in cell fate determination and proliferation. In this study we demonstrated that hASCs are endowed with active Hedgehog and BMP signaling pathways through the expression of genes of both cascades and that their expressions are downregulated after neuronal induction. This idea is in accordance with the facts that Shh and BMP signaling is involved in the maintenance of cells with stem cells properties and that proliferation decreases during the process of differentiation. Furthermore, Noggin expression was detected in induced hASCs whereas there was no expression in noninduced cells, which indicates that these cells are probably adopting a neuronal fate because noggin diverts neural stem cells from glial to neuronal fate. We also detected FM1-43 and synaptophisin staining, which is evidence of the presence of putative functional presynaptic terminals, a neuron-specific property. These results could partially contribute to the elucidation of the molecular mechanisms involved in neuronal differentiation of adult human nonneural tissues.

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Section: Changes In Hascs Gene Expressionmentioning

confidence: 58%

Differential Expression of Shh and BMP Signaling in the Potential Conversion of Human Adipose Tissue Stem Cells Into Neuron-Like Cells In Vitro

Cardozo

Ielpi²,

Gómez³

et al. 2010

gene expr

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“…Each ID protein has a distinct pattern of expression in the developing nervous system with characteristic spatial and temporal patterns in restricted cell types. For example, ID1 and ID3 are detected in dividing neuroblasts, whereas ID2 and ID4 are expressed in relatively mature neurons, and ID2 and ID3 are absent in O4 + OLs (Jen et al, 1996;Jen et al, 1997;Tzeng and de Vellis, 1998). This suggests that the cellular specificity of the Id gene family may play a critical role in lineage commitment.…”

Section: Id Proteins Act As Effectors Of Bmp4 Signalingmentioning

confidence: 99%

Interactions between ID and OLIG proteins mediate the inhibitory effects of BMP4 on oligodendroglial differentiation

2004

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Bone morphogenetic protein (BMP) signaling inhibits the generation of oligodendroglia and enhances generation of astrocytes by neural progenitor cells both in vitro and in vivo. This study examined the mechanisms underlying the effects of BMP signaling on glial lineage commitment. Treatment of cultured neural progenitor cells with BMP4 induced expression of all four members of the inhibitor of differentiation (ID) family of helix-loop-helix transcriptional inhibitors and blocked oligodendrocyte (OL) lineage commitment. Overexpression of Id4 or Id2 but not Id1 or Id3 in cultured progenitor cells reproduced both the inhibitory effects of BMP4 treatment on OL lineage commitment and the stimulatory effects on astrogliogenesis. Conversely, decreasing the levels of Id4 mRNA by RNA interference enhanced OL differentiation and inhibited the effects of BMP4 on glial lineage commitment. This suggests that induction of Id4 expression mediates effects of BMP signaling. Bacterial two-hybrid and co-immunoprecipitation studies demonstrated that ID4,and to a lesser extent ID2, complexed with the basic-helix-loop-helix transcription (bHLH) factors OLIG1 and OLIG2, which are required for the generation of OLs. By contrast, ID1 and ID3 did not complex with the OLIG proteins. In addition, the OLIG and ID proteins both interacted with the E2A proteins E12 and E47. Further, exposure of cultured progenitor cells to BMP4 changed the intracellular localization of OLIG1 and OLIG2 from a predominantly nuclear to a predominantly cytoplasmic localization. These observations suggest that the induction of ID4 and ID2, and their sequestration of both OLIG proteins and E2A proteins mediate the inhibitory effects of BMP signaling on OL lineage commitment and contribute to the generation of astrocytes.

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“…Transition genes expressed highly in the

c_{1} - c_{0}

branch include CEBPG, POU3F1, POU3F2, NR2F1, NR2F2, ARX, LIN28A, TOX3, ZBTB20, PROX1 , and SOX15 which have been previously implicated in proliferation of forebrain progenitors (Au et al, 2013; Borello et al, 2014; Cimadamore et al, 2013; Dominguez et al, 2013; Yang et al, 2015) and the migratory behaviors of ganglionic eminences (Kanatani et al, 2008; Kessaris et al, 2014; Lodato et al, 2014; Olivetti and Noebels, 2012; Reinchisi et al, 2012). The

c_{0} - c_{2}

transition genes include DMRTA1, HES1, HES5, FOXG1, PAX6, HMGA2, SOX2, SOX3, SOX9, SOX6, SP8, OTX2, TGIF, ID4, SOX3, TCF7L2 , and TCFL1 which are known to be expressed in forebrain progenitors of the developing neocortex, thalamus, and hypothalamus (Abraham et al, 2013; Pozniak et al, 2010; Shimojo et al, 2011; Tzeng and de Vellis, 1998; Wang et al, 2006), and are known to establish dorsal forebrain regional identity; (Azim et al, 2009; Bani-Yaghoub et al, 2006; Borello et al, 2014; Gaston-Massuet et al, 2016; Hagey et al, 2014; Hutton and Pevny, 2011; Johansson et al, 2013; Kikkawa et al, 2013; Kishi et al, 2012; Manuel et al, 2011; Miyoshi and Fishell, 2012; Ohtsuka et al, 2001; Ross et al, 2003; Shen and Walsh, 2005; Sur and Rubenstein, 2005; Yang et al, 2015; Zembrzycki et al, 2007). …”

Section: Resultsmentioning

confidence: 99%

Discovering sparse transcription factor codes for cell states and state transitions during development

Furchtgott

Melton

Menon

et al. 2017

eLife

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Computational analysis of gene expression to determine both the sequence of lineage choices made by multipotent cells and to identify the genes influencing these decisions is challenging. Here we discover a pattern in the expression levels of a sparse subset of genes among cell types in B- and T-cell developmental lineages that correlates with developmental topologies. We develop a statistical framework using this pattern to simultaneously infer lineage transitions and the genes that determine these relationships. We use this technique to reconstruct the early hematopoietic and intestinal developmental trees. We extend this framework to analyze single-cell RNA-seq data from early human cortical development, inferring a neocortical-hindbrain split in early progenitor cells and the key genes that could control this lineage decision. Our work allows us to simultaneously infer both the identity and lineage of cell types as well as a small set of key genes whose expression patterns reflect these relationships.DOI: http://dx.doi.org/10.7554/eLife.20488.001

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Id1, Id2, and Id3 gene expression in neural cells during development

Cited by 72 publications

References 28 publications

Differential Expression of Shh and BMP Signaling in the Potential Conversion of Human Adipose Tissue Stem Cells Into Neuron-Like Cells In Vitro

Differential Expression of Shh and BMP Signaling in the Potential Conversion of Human Adipose Tissue Stem Cells Into Neuron-Like Cells In Vitro

Interactions between ID and OLIG proteins mediate the inhibitory effects of BMP4 on oligodendroglial differentiation

Discovering sparse transcription factor codes for cell states and state transitions during development

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