Idaho ‘Cabernet Sauvignon’ grape composition altered by grapevine leafroll-associated virus 3

Lee, Jungmin; Rennaker, Christopher; Thompson, Brandon; Dahan, Jennifer; Karasev, Alexander V.

doi:10.1016/j.nfs.2023.02.002

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…Viruses are a concern for grape production in the state, with grapevine leafroll-associated virus 3 (GLRaV-3), grapevine fleck virus (GFkV), grapevine red blotch virus (GRBV), grapevine rupestris vein feathering virus (GRVFV), grapevine-associated tymo-like virus (GaTLV), and grapevine rupestris stem pitting-associated virus (GRSPaV) reported in the state over the years [ 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. Some of these virus infections negatively impact grape quality [ 20 , 21 ]. The application of high-throughput sequencing (HTS) methods greatly facilitates the detection, identification, and discovery of new viruses in grapevines, expanding the number of viruses known to infect V. vinifera [ 11 , 22 ] and the number known in new geographic areas, including many viruses not previously reported in Idaho [ 15 , 16 , 17 , 18 , 19 ].…”

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confidence: 99%

Grapevine Endophyte Endornavirus and Two New Endornaviruses Found Associated with Grapevines (Vitis vinifera L.) in Idaho, USA

Dahan

Orellana

Lee

et al. 2023

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Five virus genomes, ranging between 12.0 and 12.3 kb in length and identified as endornaviruses, were discovered through a high-throughput sequencing (HTS) analysis of the total RNA samples extracted from two wine grape cultivars collected in the state of Idaho. One was found in a declining Chardonnay vine and was determined to be a local isolate of grapevine endophyte endornavirus (GEEV), and four others represented two novel endornaviruses named grapevine endornavirus 1 (GEV1) and grapevine endornavirus 2 (GEV2). All three virus genomes span a large, single open reading frame encoding polyproteins with easily identifiable helicase (HEL) and RNA-dependent RNA polymerase (RdRP) domains, while the GEV2 polyprotein also contains a glycosyltransferase domain. The GEV1 genome found in an asymptomatic Cabernet franc vine was related to, but distinct from, GEEV: the 5′-proximal, 4.7 kb segment of the GEV1 genome had a 72% identical nucleotide sequence to that of GEEV, while the rest of the genome displayed no significant similarity to the GEEV nucleotide sequence. Nevertheless, the amino acid sequence of the RdRP domain of GEV1 exhibited the closest affinity to the RdRP of GEEV. GEV2 was found in declining Chardonnay and asymptomatic Cabernet franc vines as three genetic variants exhibiting a 91.9–99.8% nucleotide sequence identity among each other; its RdRP had the closest affinity to the Shahe endorna-like virus 1 found in termites. In phylogenetic analyses, the RdRP and HEL domains of the GEV1 and GEV2 polyproteins were placed in two separate clades inside the large lineage of alphaendornaviruses, showing an affinity to GEEV and Phaseolus vulgaris endornavirus 1, respectively.

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