IDDF2020-ABS-0034 Network pharmacology analysis to uncover the potential mechanisms of lycium barbarum on colorectal cancer

Lü, Yi; Sun, Jiachen; Hu, Minhui; Kong, Xianhe; Zhong, Weijie

doi:10.1136/gutjnl-2020-iddf.17

Cited by 1 publication

(1 citation statement)

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“…However, the active component content is replaced by the relative dosage of TCM because of the difficulty of obtaining content at the same level. The "degree" value was topologically calculated through CytoHubba (https://apps.cytoscape.org/apps/cytoHubba, version 3.7.1) [13,14]. Finally, formula (3) combined the targets of components with topology parameters to calculate the weight of the targets f(A, B) after normalization.…”

Section: Core Target Acquisitionmentioning

confidence: 99%

A systematic strategy for investigating the pharmacological effects and mechanism of traditional Chinese medicinal formula: Guilin Xiguashuang as a case

Zhang,

Ren,

Liu

et al. 2023

Fundamemntal Clinical Pharma

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BackgroundTraditional Chinese medicinal formula (TCMF) has specific advantages in treating diseases. However, the pharmacological effects and mechanism of TCMF composed of traditional Chinese medicines (TCM) with unclear active components or targets have not yet been fully elucidated.ObjectivesThis research proposed a strategy for elucidating the pharmacological effects and mechanism to address this issue systematically.MethodsWith Guilin Xiguashuang (GLXGS) taken as a case, this study newly provided the multi‐level assays, which decomposes TCMF into components, TCM, and TCMF levels. The main pharmacological effects were acquired through a comprehensive analysis based on the active components, pharmacological effects of TCM, and clinical efficacy of TCMF, respectively. The core targets and pathways were further identified and verified to elucidate the mechanism.ResultsThe main pharmacological effects of GLXGS were anti‐inflammatory, analgesic, antibacterial, immunoregulatory, and wound healing. Moreover, the mechanism analysis demonstrated that GLXGS was involved in the regulation of NF‐κB and VEGF signaling pathways and core targets, such as IL‐6 and TNF‐α. Finally, unproven immunomodulatory and anti‐inflammatory mechanism were verified using RAW264.7 and THP‐1 cells. GLXGS was verified to down‐regulate IL‐6, IL‐1β, TNF‐α, and CD86 in lipopolysaccharides‐stimulated RAW264.7 cells, while enhancing polarization in both RAW264.7 and THP‐1 cells, which were consistent with analysis results.ConclusionThe present research provides a systematic strategy for the pharmacological effect prediction and mechanism analysis of TCMF, which is of great significance for studying complex TCMF.

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Section: Core Target Acquisitionmentioning

confidence: 99%