Identical embryonal proteins in intact and isolated tissues of maize (Zea mays L.)

Abstract

Minor antigens characteristic of developing and mature embryos were not found in the shoot and root meristems of the seedlings. Some of these embryonal antigens (EA) were present, however, in callus and cell-suspension cultures, irrespective of their tissue origin, and were maintained throughout repeated subcultures, in some cases for more than 2 years. These EA were distinct both from the meristematic antigens found in the intact seedlings and in callus cultures, and from organ-specific antigens found only in intact plants. The EA of callus tissues derived from several maize genotypes were serologically identical. We therefore assume that these EA are "proliferation proteins" or "early proteins" expressed by cells that have not undergone any determination and lack any tissue or organ specificity.

Cloning of genes developmentally regulated during plant embryogenesis

“…However, the second point is that nearly all of the embryonic antigens are also present in the unorganized cell clusters. Substantially similar findings have been reported in zygotic embryos of maize, where embryonal antigens are absent in seedling root and shoot meristems but present in callus and cell-suspension cultures (26). The results also corroborate the two-dimensional gel data on protein synthesis patterns (6).…”

Isolation and partial characterization of two antigenic glycoproteins from rye-grass (Lolium perenne) pollen

“…These glycoproteins are of interest from several points of view: firstly, macromolecules of the pollen wall have been implicated in the recognition of pollen by a compatible stigma (Heslop-Harrison, 1975); the glycoproteins studied are present in the pollen wall (Knox et al, 1980) and may be involved in the pollen-stigma recognition. Secondly, both glycoproteins are effective antigens in rabbits; increasingly, immunological techniques are being used to demonstrate markers of identity in plants (Raff et al, 1980) and to monitor molecular changes associated with differentiation (Khavkin et al, 1977(Khavkin et al, , 1979, although the antigens involved have not been chemically defined. The present study contributes to our understanding of the nature of antigenic plant macromolecules.…”