Identical independent sites for dye ligand on bovine serum albumin demonstrated by multivariate analysis

The complex between 9-(n-decanyl)acridone and Bovine Serum Albumin. Part 2. What do fluorescence probes probe?

“…Different probes have been used successfully with BSA, including ANS, 9-11 11-((5-dimethylaminonaphthalene-1-sulfonyl)amino)undecanoic acid (DAUDA), 12 5-dimethylaminonaphthalene-1-sulfonyl chloride (dansyl-Cl), 12,13 8-amino-2-[(2amino-5-methylphenoxy)methyl]-6-methoxyquinoline-N,N,NЈ, NЈ-tetraacetic acid, tetrapotassium salt (Quin-2), 14 acrylodan intestinal fatty acid binding protein (ADIFAB), 15 Tb(), 16 eosin B and eosin Y, 17 5-(2Ј-carboxyphenyl)azoquinolin-8-ol, 18 bromocresol purple, 19 N-oleylethanolamine, 20 Coomassie brilliant blue, 21 sodium dodecyl sulfate, cethyltrimethylammonium chloride and N-hexadecyl-N,N-dimethyl-3-ammonium-1propanesulfonate 22 or even probes covalently bound 23 to BSA. Direct intrinsic fluorescence of BSA was also used 24-27 and the energy transfer 28 between an intrinsic chromophore and an external probe was studied.…”

Donor-Acceptor Conjugated Linear Polyenes: A Study of Excited State Intramolecular Charge Transfer, Photoisomerization and Fluorescence Probe Properties

“…Most of the known extrinsic fluorescence probes such as ANS [93], Nile red [94], cyanine dye and rhodamine [95], brilliant blue [96], bromocresol purple anion [97], acridone derivatives [98], non-cyanine fluorescence probe [99], etc. are ionic, polar and hydrophilic in nature.…”

Fluorescence probing and molecular docking analysis of the binding interaction of bovine serum albumin (BSA) with the polarity probe AICCN

“…The binding of such probes to binding sites of the albumin proteins is well documented. [1][2][3][4][5][6][7][8] The albumin proteins are important for their endogenous and exogenous transport properties. 9,10 The secondary structure of these proteins involves about 67% helical structures consisting of 6 turns and 17 disulfide bridges.…”