Identificación de Arcobacter en heces de niños y adultos con/sin diarrea y en reservorios animales

Larrauri, Rito Zerpa; Villaverde, Jorge O. Alarcón; Vigo, Percy E. Lezama; Gabriel, L. Patiño; Dioses, Alberto Reyes; Ramírez, Augusto M. Valencia; Velásquez, Jorge Néstor; Sevilla-Andrade, Carlos Raúl; León, Miriam J. Alarcón

doi:10.15381/anales.v75i2.8389

Cited by 3 publications

(3 citation statements)

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“…Notwithstanding, reports in Peru on the pathogenicity of this genus are scarce, e.g. we found only one published study that mentioned Arcobacter in stool from children with diarrhea [44]. In particular, Arcobacter cryaerophilus, the most frequent ASV in the Metropolitan area, has been associated with severe diarrhea and found to carry several virulence genes [45].…”

Section: Discussionmentioning

confidence: 80%

From the Andes to the desert: First overview of the bacterial community in the Rimac river, the main source of water for Lima, Peru

Romero

Calla-Quispe

Castillo-Vilcahuaman

et al. 2020

Preprint

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BackgroundThe Rimac river is the main source of water for Lima, Peru’s capital megacity. The river is constantly affected by different types of contamination including mine tailings in the Andes and urban sewage in the metropolitan area. We aim to produce the first characterization of bacterial communities in the Rimac river using a 16S rRNA amplicon sequencing approach which would be useful to identify bacterial diversity and potential understudied pathogens.ResultsWe report a higher diversity in bacterial communities from the Upper and, especially, Middle Rimac compared to the Lower Rimac (Metropolitan zone). Samples were generally grouped according to their geographical location. Bacterial classes Alphaproteobacteria, Bacteroidia, Campylobacteria, Fusobacteriia, and Gammaproteobacteria were the most frequent along the river in different proportions. Arcobacter cryaerophilus (Campylobacteria) was the most frequent species in the Lower Rimac while an unidentified Leptotrichiaceae (Fusobacteriia) and Flavobacterium succinicans (Bacteroidia) were the most predominant in the Upper zone. Predicted metabolic functions in the microbiota include bacterial motility, quorum sensing and xenobiotics metabolism. Additional metabolomic analyses showed the presence natural flavonoids and antibiotics in the Upper Rimac and herbicides in the Lower Rimac.ConclusionsThe dominance in the Metropolitan area of Arcobacter cryaerophilus, an emergent pathogen associated with fecal contamination and antibiotic multiresistance, but that is not usually reported in traditional microbiological quality assessments, highlights the necessity to apply next-generation sequencing tools to improve pathogen surveillance. We believe that our study will the integration of omics sciences in Peru and its application on current environmental and public health issues.Ethical approvalThe study did not involve experiments in humans nor animal subjects.

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Section: Discussionmentioning

confidence: 80%

From the Andes to the desert: First overview of the bacterial community in the Rimac river, the main source of water for Lima, Peru

Romero

Calla-Quispe

Castillo-Vilcahuaman

et al. 2020

Preprint

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“…Notwithstanding, reports in Peru on the pathogenicity of this genus are scarce, e.g. we found only one published study that mentioned Arcobacter in stool from children with diarrhea [59].…”

Section: Discussionmentioning

confidence: 81%

From the Andes to the desert: 16S rRNA metabarcoding characterization of aquatic bacterial communities in the Rimac river, the main source of water for Lima, Peru

et al. 2021

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The Rimac river is the main source of water for Lima, Peru’s capital megacity. The river is constantly affected by different types of contamination including mine tailings in the Andes and urban sewage in the metropolitan area. In this work, we aim to produce the first characterization of aquatic bacterial communities in the Rimac river using a 16S rRNA metabarcoding approach which would be useful to identify bacterial diversity and potential understudied pathogens. We report a lower diversity in bacterial communities from the Lower Rimac (Metropolitan zone) in comparison to other sub-basins. Samples were generally grouped according to their geographical location. Bacterial classes Alphaproteobacteria, Bacteroidia, Campylobacteria, Fusobacteriia, and Gammaproteobacteria were the most frequent along the river. Arcobacter cryaerophilus (Campylobacteria) was the most frequent species in the Lower Rimac while Flavobacterium succinicans (Bacteroidia) and Hypnocyclicus (Fusobacteriia) were the most predominant in the Upper Rimac. Predicted metabolic functions in the microbiota include bacterial motility and quorum sensing. Additional metabolomic analyses showed the presence of some insecticides and herbicides in the Parac-Upper Rimac and Santa Eulalia-Parac sub-basins. The dominance in the Metropolitan area of Arcobacter cryaerophilus, an emergent pathogen associated with fecal contamination and antibiotic multiresistance, that is not usually reported in traditional microbiological quality assessments, highlights the necessity to apply next-generation sequencing tools to improve pathogen surveillance. We believe that our study will encourage the integration of omics sciences in Peru and its application on current environmental and public health issues.

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“…e camarões(Larrauri et al, 2014).De acordoShah et al 2011, a carne de frango foi relatada com maior prevalência para Arcobacter spp., seguida por carne suína e bovina. Diferentes taxas de prevalência foram registradas em amostras de carne de frango na Índia variando de 12% a 33%(Patyal et al, 2011;Mohan et al, 2014; Ramees et al, 2014b).Recentemente, diferentes espécies de Arcobacter foram isoladas (11% das amostras examinadas) de aves de capoeira na Costa Rica(Bogantes et al, 2015).…”

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Ocorrência de <i>Arcobacter</i> spp. em carne de frango

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Occurence of Arcobacter spp. in poultry meat Arcobacter spp., previously known as aerotolerant Campylobacter, is considered a bacterial genus that includes species considered emerging pathogens that can be transmitted by food. Arcobacter has been associated with gastroenteritis, persistent diarrhea and bacteremia in humans. It is a gram negative, thermosensitive bacterium, although it can survive at 4 o C. In Brazil, there are few studies of the occurrence of Arcobacter in animal products including those of animal origin, especially those most consumed, such as poultry and pork. There are occasional studies of their occurrence in cooled products, such as cuts and in refrigerated chicken carcasses. The objective of this study was to evaluate the occurrence of Arcobacter spp. in refrigerated chicken cuts and carcasses of the retail and in chicken thighs free of antibiotic and organic refrigerated from slaughterhouse by conventional isolation and genotyping by polymerase chain reaction (PCR). A total of 153 chicken meat samples were analyzed, of which 39.21% (59/153) were positive for the Arcobacter genus. A total of sixty-four isolates positive for Arcobacter spp., corresponding 89.06% (57/64) of A. lacus, 4.7% (3/64) of A. thereius, 3.12% (2/64) of A. butzleri, and 3.12% (2/64) of Arcobacter spp. species not yet identified. Phenotypic resistance analyzes were performed on 12 antibiotics with 34 isolates, previously selected from four different sources of chicken meat obtained in this study, and three strains used as positive control of Arcobacter spp. Phenotypic resistance to antimicrobials were 100% for nalidixic acid and clindamycin , 29.73% for erythromycin, 24.32% for kanamycin, 21.62% for tetracycline, 18.42% for chloramphenicol, 13.51% for gentamycin, 8.11% for streptomycin, 5.41% for azithromycin and ciprofloxacin, 2.10% for vancomycin and 0.00% for ampicillin.

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Identificación de Arcobacter en heces de niños y adultos con/sin diarrea y en reservorios animales

Cited by 3 publications

References 1 publication

From the Andes to the desert: First overview of the bacterial community in the Rimac river, the main source of water for Lima, Peru

From the Andes to the desert: First overview of the bacterial community in the Rimac river, the main source of water for Lima, Peru

From the Andes to the desert: 16S rRNA metabarcoding characterization of aquatic bacterial communities in the Rimac river, the main source of water for Lima, Peru

Ocorrência de <i>Arcobacter</i> spp. em carne de frango

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