Identificación de diferencias proteómicas en muestras oligozoospérmicas

Botta, Teresa García; Blescia, Sabrina; Martínez-Heredia, Juan; Lafuente, Rafael; Brassesco, M.; Ballescà, Josep Lluís; Oliva, Rafael

doi:10.1016/s1698-031x(09)70257-2

Cited by 5 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the relationship between GSTM3 content and mitochondrial activity found in the present study strengthens the hypothesis of a tight molecular relationship between sperm GSTs and mitochondrial function. Moreover, GSTM3 is clearly related to sperm quality, as it has been established as a quality [42][43][44], fertility [16], and cryotolerance [17] biomarker in both boar and human sperm. Hence, one could suggest that the GSTM3 content in fresh boar semen may be used as a biomarker of sperm quality during liquid preservation.…”

Section: Discussionmentioning

confidence: 99%

Glutathione S-Transferases Play a Crucial Role in Mitochondrial Function, Plasma Membrane Stability and Oxidative Regulation of Mammalian Sperm

et al. 2020

View full text Add to dashboard Cite

Glutathione S-transferases (GSTs) are essential sperm antioxidant enzymes involved in cell protection against oxidative stress and toxic chemicals, preserving sperm function and fertilising ability. Artificial insemination (AI) in pigs is commonly carried out through the use of liquid-stored semen at 17 °C, which not only reduces sperm metabolic activity but also sperm quality and AI-farrowing rates within the 72 h of storage. While one may reasonably suggest that such enzymes are implicated in the physiology and maintenance of mammalian sperm function during liquid-storage, no previous studies conducted on any species have addressed this hypothesis. Therefore, the objective of the present work was to characterise the presence and function of sperm GSTs in mammalian sperm, using the pig as a model. In this regard, inhibition of such enzymes by ethacrynic acid (EA) during semen storage at 17 °C was performed to evaluate the effects of GSTs in liquid-preserved boar sperm by flow cytometry, immunofluorescence, and immunoblotting analysis. The results of this study have shown, for the first time in mammalian species, that the inhibition of GSTs reduces sperm quality and functionality parameters during their storage at 17 °C. These findings highlight the key role of such enzymes, especially preserving mitochondrial function and maintaining plasma membrane stability. In addition, this study has identified and localised GSTM3 in the tail and equatorial subdomain of the head of boar sperm. Finally, this study has set grounds for future investigations testing supplementation of semen extenders with GSTs, as this may improve fertility outcomes of swine AIs.

show abstract

Section: Discussionmentioning

confidence: 99%

Glutathione S-Transferases Play a Crucial Role in Mitochondrial Function, Plasma Membrane Stability and Oxidative Regulation of Mammalian Sperm

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Higher GSTM3-content before cryopreservation was related to a lower percentage of viable spermatozoa (SYBR14 + /PI − ), viable spermatozoa with low membrane disorder, viable spermatozoa with low levels of intracellular superoxide levels, and spermatozoa with low mitochondrial membrane potential at post-thaw. Interestingly, other studies have demonstrated that an overexpression of GSTM3 in pre-frozen sperm is related with small litter sizes in boars [21] and with lower sperm quality in humans [33]. In addition, Hemachand and Shaha [20] reported that membrane-bound GSTMs eliminate ROS via extracellular glutathione and, consequently, prevent lipid membrane peroxidation, a process highly damaging to sperm membrane integrity [34].…”

Section: Discussionmentioning

confidence: 99%

GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm

Llavanera

Delgado-Bermúdez

Fernández-Fuertes

et al. 2019

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Background Cryopreservation is currently the most efficient method for long-term preservation of mammalian gametes and is extensively used in swine artificial insemination (AI) centres. However, it is well-known that cryopreservation procedures induce changes in the water phase in both intra and extracellular compartments, which alter the content and localisation of several proteins and ends up curtailing the structural integrity of functional sperm (i.e., cryoinjuries). Alterations and deficiencies of sperm-oocyte binding proteins during gamete recognition are one of the causes of reproductive failure both in vitro and in vivo . In this sense, characterisation of cryopreservation effects upon oocyte-binding proteins of sperm, such as IZUMO1 and GSTM3, is essential when assessing the impact of this technique in swine reproduction. Results Cryopreservation was found to induce changes in the localisation of IZUMO1 and GSTM3 in boar sperm. However, the relative content of both proteins was not altered after thawing. Furthermore, whereas IZUMO1 content was found not to be related to the cryotolerance of boar sperm, GSTM3 content was observed to be higher in poor (PFE) than in good (GFE) freezability ejaculates in both pre-frozen (1.00 INT·mm 2 ± 0.14 INT·mm 2 vs. 0.72 INT·mm 2 ± 0.15 INT·mm 2 ; P < 0.05) and post-thawed (0.96 INT·mm 2 ± 0.20 INT·mm 2 vs. 70 INT·mm 2 ± 0.19 INT·mm 2 ; P < 0.05) samples. Moreover, GSTM3 levels were found to be higher in those spermatozoa that exhibited low mitochondrial activity, high reactive oxygen species (ROS) production, and high membrane lipid disorder post-thaw ( P < 0.05). Conclusions The difference in GSTM3 content between GFE and PFE, together with this protein having been found to be related to poor sperm quality post-thaw, suggests that it could be used as a cryotolerance marker of boar spermatozoa. Furthermore, both IZUMO1 and GSTM3 relocate during cryopreservation, which could contribute to the reduced fertilising capacity of frozen-thawed boar sperm. Electronic supplementary material The online version of this article (10.1186/s40104-019-0370-5) contains supplementary material, which is available to authorized users.

show abstract