Identification and age-dependance of pteridines in the head of adult Mexican fruit fly, Anastrepha ludens

Tomic-Carruthers, Nada; Robacker, David C.; Mangan, Robert L.

doi:10.1016/0022-1910(95)00116-6

Cited by 10 publications

(12 citation statements)

References 25 publications

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“…Excitation and emission wavelengths were 357 and 450 nm, respectively. Several pteridines contribute to the total ßuorescence measured in dipteran head capsules (Tomic-Carruthers et al 1996). In our study, the amount of pteridine extracted from each ßy head was calculated by using the average of three pterin (Aldrich, Milwaukee, WI) standard curve equations.…”

Section: Nirs Data Collectionmentioning

confidence: 99%

“…Lysyk and Krafsur (1993) reported that the rate of pteridine accumulation was a function of temperature, suggesting that for this method to be accurately applied to Þeld-collected ßies, the effects of temperature on the accumulation rate had to be considered and the temperatures to which the ßies had been exposed needed to be known. The pteridine method of age determination has been used in studies with other ßies, including tse-tse ßies (Lehane and Mail 1985); face ßies, Musca autumnalis De Geer ; blow ßies, Lucilia sericata Meigen (Wall et al 1991); horn ßies, Hematobia irritans (L.) (Krafsur et al 1992); house ßies, Musca domestica L. (McIntyre and Gooding 1995); Mediterranean fruit ßies, Ceratitis capitata (Weidemann) (Camin et al 1991); and Mexican fruit ßies, Anastrepha ludens (Loew) (Tomic-Carruthers et al 1996). Although the pteridine ßuorescence technique is relatively simple, it is time consuming and requires several laboratory instruments that cannot readily be taken to the Þeld.…”

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confidence: 99%

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Chronological Age-Grading of House Flies by Using Near-Infrared Spectroscopy

Perez-Mendoza¹,

Dowell

Broce

et al. 2002

J Med Entomol

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The sensitivity and accuracy of near-infrared spectroscopy (NIRS) was compared with that of the pteridine fluorescence technique for estimating the chronological age of house flies, Musca domestica (L.). Although results with both techniques were significantly correlated with fly age, confidence limits on predicted ages generally were smaller with NIRS. Young flies could be readily differentiated from old flies by using NIRS. Age predictions using the pteridine method are dependent upon size, sex, and temperature at which adult flies are exposed. In contrast, those factors do not need to be determined for age-grading using NIRS. Classification accuracy using the NIRS method was similar for whole flies, fresh heads, dried heads, and ethanol-preserved heads. The NIRS method was also suitable for predicting age of stable flies, Stomoxys calcitrans (L.), and face flies, Musca autumnalis De Geer. NIRS has several advantages over the measurement of pteridine levels for age-grading field-collected flies, including speed and portability of instrumentation, and not needing to determine sex, size, and temperatures to which adult flies were exposed.

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Section: Nirs Data Collectionmentioning

confidence: 99%

mentioning

confidence: 99%

Chronological Age-Grading of House Flies by Using Near-Infrared Spectroscopy

Perez-Mendoza¹,

Dowell

Broce

et al. 2002

J Med Entomol

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“…Studies of the relationship between pteridine concentration and age are much more taxonomically limited than those of lipofuscins, being restricted almost entirely to various dipteran families, such as the Calliphoridae (Wall et al 1991), Culicidae (Penilla et al 2002), Drosophilidae (Robson et al 2006), Muscidae (McIntyre & Gooding 1995, Simulidae (Millest et al 1992) and Tephritidae (Tomic-Carruthers et al 1996). The presence of pteridines in any social insect remains to be examined.…”

Section: Introductionmentioning

confidence: 97%

An evaluation of two biochemical methods of age determination in insects (pteridines and lipofuscins) using the ant Polyrhachis sexpinosa Latrielle (Hymenoptera: Formicidae)

Robson

Crozier

2009

Australian Journal of Entomology

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Accurate information on the age of wild-caught animals is valuable for a variety of areas, but can be particularly difficult to obtain for small holometabolous insects, whose body size is fixed at the time of pupal eclosion. A variety of chemical groups, such as lipofuscins and pteridines accumulate in body tissues through time and can be used to predict age in a variety of arthropod taxa. Here we use spectrofluorometry to confirm the presence of extractable levels of lipofuscins and pteridines in individual social insects (using the ant Polyrhachis sexpinosa Latrielle, average body size 25 mg, as an example) and evaluate their ability to predict age. Pteridine levels were independent of age but lipofuscin levels increased with age in a predictable manner (r 2 = 72.8%). Lipofuscin levels therefore represent a new method of age determination for social insects that should be applicable to both individual laboratory and wild-caught animals.

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“…NIRS was first used for age‐grading short‐lived house flies, Musca domestica L (Diptera: Muscidae) 95. The method was more accurate than the standard method of measuring pteridine accumulation in the heads of Diptera 96. This technology has also been successfully applied to long‐lived stored‐product beetles (Perez‐Mendoza, Throne, Dowell and Baker, unpublished data) and to cockroaches (Perez‐Mendoza, Nalyanya, Dowell, Schal, Throne and Baker, unpublished data).…”

Section: Research Highlightsmentioning

confidence: 99%

Post‐harvest entomology research in the United States Department of Agriculture–Agricultural Research Service

Throne

Hallman

Johnson

et al. 2003

Pest Management Science

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This is a review of current post-harvest entomology research conducted by the Agricultural Research Service, the research branch of the US Department of Agriculture. The review covers both durable and perishable commodities. Research on biochemistry, genetics, physiology, monitoring and control of insects infesting stored grain, dried fruits and nuts, and processed commodities is reviewed. Research on development of quarantine treatments, particularly for fruit flies, is also reviewed, including research on thermal and irradiation treatments and a discussion of risk management for quarantine pests. Two areas of research are covered more extensively: a project to map the genome of the red flour beetle, Tribolium castaneum, and the use of near-infrared spectroscopy for detection of hidden infestations in grain, quantification of insect fragments in food, determination of quality in dried fruits, identification of insect species and age-grading insects. Future research directions are identified.

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Identification and age-dependance of pteridines in the head of adult Mexican fruit fly, Anastrepha ludens

Cited by 10 publications

References 25 publications

Chronological Age-Grading of House Flies by Using Near-Infrared Spectroscopy

Chronological Age-Grading of House Flies by Using Near-Infrared Spectroscopy

An evaluation of two biochemical methods of age determination in insects (pteridines and lipofuscins) using the ant Polyrhachis sexpinosa Latrielle (Hymenoptera: Formicidae)

Post‐harvest entomology research in the United States Department of Agriculture–Agricultural Research Service

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