Identification and analysis of a differentially expressed wheat RING-type E3 ligase in spike primordia development during post-vernalization

Functional characterization of wheat histone H2B monoubiquitination enzyme TaHUB2 in response to vernalization in Keumkang (Triticum aestivum L.)

“…TaHUB2 exhibited self-ubiquitination activities (Figure 3(B)) and was degraded in cell-free extracts (Figure 4(B)). In our previous study, we found that the heading time was not differ between spring and winter types if they were vernalized about 50 days (Kim et al 2021). Therefore, 50 days of vernalization is enough to synchronize heading time regardless of vernalized requirement.…”

Molecular characterization of wheat floret development‐related F‐box protein (TaF‐box2): Possible involvement in regulation of Arabidopsis flowering

“…The reaction contained His‐Trx‐TaCYCL1, His‐AtUBC10, ubiquitin (Sigma‐Aldrich), human E1 (Sigma‐Aldrich), human CUL1/RBX1 (BostonBiochem), human SKP1 (BostonBiochem), and ubiquitination buffer (1 M Tris–HCl, pH 7.5; 40 mM ATP; 100 mM MgCl 2 ; 40 mM dithiothreitol [DTT]). The ubiquitination assay method were previously described by Kim, Khan, et al (2021).…”

“…Each different stage of wheat primordia and floret measurement and sampling information were elucidated as previously described (Kim, Khan, et al, 2021).…”

“…Floral transition, a phase change from the vegetative to the reproductive phase, is essential in plant organ development (Araki, 2001), and it mainly occurs when there is a change in the progress of the shoot in response to stimuli that promote reproductive development (Kim, Khan, et al, 2021). In Arabidopsis , inductive stimuli in leaves promote flowering and lead to floral transition.…”