Identification and analysis of a processed cytochrome P450 pseudogene of the disease vector  Aedes aegypti

El-garj, Fatma M. A.; Wajidi, Mustafa Fadzil Farid; Avicor, Silas Wintuma

doi:10.1016/j.apjtm.2016.07.024

Cited by 3 publications

(7 citation statements)

References 21 publications

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“…Clans are defined "as groups of P450 families that consistently cluster together on a phylogenetic tree'' [17]. These are higher-order groups and are basically similar to clades, although clades technically refer to species with a common ancestor and not to sequences [18]. The insect CYP6 and CYP9 families belong in a clan with vertebrate CYP3 and CYP5.…”

Section: P450 Clansmentioning

confidence: 99%

“…This has been named the CYP3 clan for the lowest family number in the group. Insects have four clans comprising CYP2, CYP3, CYP4, and mitochondrial CYP11, CYP24, CYP27 families [18].…”

Section: P450 Clansmentioning

confidence: 99%

“…In Anopheles gambiae genome alone, there are 111 annotated Cytochromes P450 [5], seven of which are pseudo genes [18]. Of these P450s, CYP314A1, CYP12F1 and CYP6Z1 are involved in the metabolism of DDT in A. gambiae in Tanzania and Zanzibar (Eastern Africa) and Western Kenya (Eastern Africa) respectively [11,12,[23][24][25].…”

Section: Regional Distribution In the African Continentmentioning

confidence: 99%

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Cytochrome P450s in Anopheles gambiae (Diptera: Culicidae) and Insecticide Resistance in Africa: A Mini Review

Mohammed¹,

Mailafia²,

Malang³

et al. 2017

Entomol Ornithol Herpetol

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Cytochrome P450s are known to be critical for the detoxification and/or activation of xenobiotics such as insecticides in all living organisms including Anopheles gambiae. Many studies have demonstrated the role of P450s in insecticide resistance in A. gambiae. However, little is known about the impact of distribution in the African subcontinent. In this paper therefore, we analyse the P450 clans, the CYP6 family, localisation and function of A. gambiae CYPs, their insecticide substrates, regional distribution in the African continent and their role in insecticide resistance. This investigation from published data revealed that in the Central region; CYP6Z3, CYP6Z1, CYP12F2, CYP6P4, CYP6GA1, CYP6Z3 (Yaoundé, Cameroun) have bendiocarb, DDT and pyrethroids as substrates; in the Eastern region: CYP314A1 and CYP12F1 (Tanzania and Zanzibar) have DDT as a substrate, CYP32A3, CYP6Z1 and CYP6Z2 (Western Kenya) have DDT, carbaryl and permethrin; whilst in the Western region: CYP6AG1, CYP6Z2, CYP6Z3, CY6P3, CYP6P4, CYP6M2 (Ghana), CYP6M2, CYP6P3 (Benin), CYP325A3, CYP6P3 and CYP6M2 (Nigeria) all have DDT, carbaryl, permethrin, trans-and cis-permethrin, deltamethrin, bendiocarb as substrates. Additionally, CYP6M2, CYP6P3, CYP6Z3 (Côte d'Ivoire), CYP6P3, CYP6Z2 and CYP9J5 (Burkina Faso) have bendiocarb, DDT plus pyrethroids and only pyrethroids as substrates respectively. Interestingly, CYP6P3 is observed to metabolize all the available insecticides (DDT, pyrethroid, trans-and cis-permethrin, deltamethrin and bendiocarb), indicating possible insecticide cross resistance across all the three regions of Africa. A more detailed understanding of the substrate specificities of various P450s and the geographical distribution of insecticide resistance in Africa is quintessential for an effective resistance management.

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Section: P450 Clansmentioning

confidence: 99%

“…This has been named the CYP3 clan for the lowest family number in the group. Insects have four clans comprising CYP2, CYP3, CYP4, and mitochondrial CYP11, CYP24, CYP27 families [18].…”

Section: P450 Clansmentioning

confidence: 99%

Section: Regional Distribution In the African Continentmentioning

confidence: 99%

See 1 more Smart Citation

Cytochrome P450s in Anopheles gambiae (Diptera: Culicidae) and Insecticide Resistance in Africa: A Mini Review

Mohammed¹,

Mailafia²,

Malang³

et al. 2017

Entomol Ornithol Herpetol

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“…In the analysis of secondary structure, the component content of the secondary structure is α-helix > random coil > extension chain > β-turn angle from large to small, and the α-spiral and random coil are also the majority in temperate bed bugs. Seven of P450 protein families have signal peptides, and only one of the N-terminals of beet armyworms [ 28 ], but none of them have signal peptides in the Aedes aegypti mosquitoes [ 29 ], indicating that there are secreted proteins in the P450 protein family of the long-lived C. buqueti , and the other two substances do not contain secreted proteins, which are synthesized by free ribosomes, proteins that enter cytosols, and participate in the chemical reactions of mitochondria, nuclei, and peroxidase bodies.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification, molecular evolution and gene expression of P450 gene family in Cyrtotrachelus buqueti

Fu,

Yang,

Long

et al. 2024

BMC Genomics

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Background Insect Cytochrome P450 monooxygenase (CYPs or P450s) plays an important role in detoxifying insecticides, causing insect populations to develop resistance. However, the molecular functions of P450 gene family in Cyrtotrachelus buqueti genome are still lacking. Results In this study, 71 CbuP450 genes have been identified. The amino acids length of CbuP450 proteins was between 183 aa ~ 1041 aa. They are proteins with transmembrane domains. The main component of their secondary structure is α-helix and random coils. Phylogenetic analysis showed that C. buqueti and Rhynchophorus ferrugineus were the most closely related. This gene family has 29 high-frequency codons, which tend to use A/T bases and A/T ending codons. Gene expression analysis showed that CbuP450_23 in the female adult may play an important role on high temperature resistance, and CbuP450_17 in the larval may play an important role on low temperature tolerance. CbuP450_10, CbuP450_17, CbuP450_23, CbuP450_10, CbuP450_16, CbuP450_20, CbuP450_23 and CbuP450_ 29 may be related to the regulation of bamboo fiber degradation genes in C. buqueti. Protein interaction analysis indicates that most CbuP450 proteins are mainly divided into three aspects: encoding the biosynthesis of ecdysteroids, participating in the decomposition of synthetic insecticides, metabolizing insect hormones, and participating in the detoxification of compounds. Conclusions We systematically analyzed the gene and protein characteristics, gene expression, and protein interactions of CbuP450 gene family, revealing the key genes involved in the stress response of CbuP450 gene family in the resistance of C. buqueti to high or low temperature stress, and identified the key CbuP450 proteins involved in important life activity metabolism. These results provided a reference for further research on the function of P450 gene family in C. buqueti.

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“…However, in past decades, almost all of the studies on the effect on mosquitoes caused by allethrin or allethrin-containing mosquito coils have focused on their activities on knockdown and/or lethality. [23][24][25][26][27][28] Whether allethrin and/or bioallethrin evokes spatial repellency in mosquitoes and the mechanism underlying spatial repellency remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Bioallethrin activates specific olfactory sensory neurons and elicits spatial repellency in Aedes aegypti

Valbon

Andreazza

Oliveira

et al. 2021

Pest Management Science

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BACKGROUND: Use of pyrethroid insecticides is a pivotal strategy for mosquito control globally. Commonly known for their insecticidal activity by acting on voltage-gated sodium channels, pyrethroids, such as bioallethrin and transfluthrin, are used in mosquito coils, emanators and other vaporizers to repel mosquitoes and other biting arthropods. However, whether specific olfactory receptor neurons are activated by pyrethroids to trigger spatial repellency remains unknown.RESULTS: We used behavioral and electrophysiological approaches to elucidate the mechanism of bioallethrin repellency in Aedes aegypti, a major vector of dengue, yellow fever, Zika and chikungunya viruses. We found that bioallethrin elicits spatial (i.e. non-contact) repellency and activates a specific type of olfactory receptor neuron in mosquito antennae. Furthermore, bioallethrin repellency is significantly reduced in a mosquito mutant of Orco, an obligate olfactory co-receptor that is essential for the function of odorant receptors (Ors). These results indicate that activation of specific Or(s) by bioallethrin contributes to bioallethrin repellency. In addition, bioallethrin repellency was reduced in a pyrethroid-resistant strain that carries two mutations in the sodium channel gene that are responsible for knockdown resistance (kdr) to pyrethroids, indicating a role of activation of sodium channels in bioallethrin repellency.CONCLUSION: Results from this study show that bioallethrin repellency is likely to be the result of co-activation of Or(s) and sodium channels. These findings not only contribute to our understanding of the modes of action of volatile pyrethroids in spatial repellency, but also provide a framework for developing new repellents based on the dual-target mechanism revealed.

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Identification and analysis of a processed cytochrome P450 pseudogene of the disease vector Aedes aegypti

Cited by 3 publications

References 21 publications

Cytochrome P450s in Anopheles gambiae (Diptera: Culicidae) and Insecticide Resistance in Africa: A Mini Review

Cytochrome P450s in Anopheles gambiae (Diptera: Culicidae) and Insecticide Resistance in Africa: A Mini Review

Genome-wide identification, molecular evolution and gene expression of P450 gene family in Cyrtotrachelus buqueti

Bioallethrin activates specific olfactory sensory neurons and elicits spatial repellency in Aedes aegypti

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