Identification and Antibioresistance Characterisation of Culturable Bacteria in the Intestinal Microbiota of Mosquitoes

Rs, Yerbanga; Fofana, Aminata; Gendrin, Mathilde; Sangaré, Ibrahim; Sanou, Soufiane; Ouangraoua, Soumeya; Af, Somé; Drabo, Aly; Simporé, Jacques; Lefèvre, Thierry; Cohuet, Anna; Christophides, George K.; Ouedraogo, J. B.

doi:10.4172/2473-4810.1000123

Cited by 2 publications

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“…gambiae s.s. in Burkina Faso. We found highly variable proportion of resistant strains between the 9 tested drugs, from 0% (8/8) for erythromycin to 76% (13/17) for ampicillin ( Yerbanga et al., 2017 ). An amoxicillin/clavulanic acid cocktail showed intermediate growth inhibition efficiency, as 41% of our isolates (7/17) was resistant to this cocktail.…”

Section: Introductionmentioning

confidence: 80%

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Analyzing gut microbiota composition in individual Anopheles mosquitoes after experimental treatment

et al. 2021

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The microbiota of Anopheles mosquitoes influences malaria transmission. Antibiotics ingested during a blood meal impact the mosquito microbiome and malaria transmission, with substantial differences between drugs. Here, we assessed if amoxicillin affects the gut mosquito microbiota. We collected Anopheles larvae in Burkina Faso, kept them in semi-field conditions, and offered a blood meal to adult females. We tested the impact of blood supplementation with two alternative amoxicillin preparations on microbiota composition, determined by highthroughput sequencing in individual gut samples. Our analysis detected four major genera, Elizabethkingia, Wigglesworthia, Asaia, and Serratia. The antibiotic treatment significantly affected overall microbiota composition, with a specific decrease in the relative abundance of Elizabethkingia and Asaia during blood digestion. Besides its interest on the influence of amoxicillin on the mosquito microbiota, our study proposes a thorough approach to report negativecontrol data of high-throughput sequencing studies on samples with a reduced microbial load.

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Section: Introductionmentioning

confidence: 80%

“…gambiae s.l. mosquitoes kept in semi-field conditions and noted that Asaia and Serratia , which belong to Proteobacteria, were predominant ( Yerbanga et al., 2017 ). Elizabethkingia has been reported by several studies as part of the microbiota of field-collected mosquitoes.…”

Section: Discussionmentioning

confidence: 99%