Identification and antibiotic resistance of Bacillus spp. isolates from natural samples

Berić, Tanja; Biočanin, Marjan; Stanković, Slaviša; Dimkić, Ivica; Janakiev, Tamara; Fira, Djordje; Lozo, Jelena

doi:10.2298/abs180302019b

Cited by 8 publications

(8 citation statements)

References 37 publications

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“…The spread of antibiotic resistance genes is an increasing problem, and it is also indicated that clinically relevant resistance could be detected in nonpathogenic bacterial strains as in the case of different Bacillus spp. strains originating from natural ecosystems (Berić et al., 2018 ). The occurrence of the B. cereus group as opportunistic pathogen has been associated with vegetables.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance pattern of the allochthonous bacteria isolated from commercially available spices

György

Laslo

Antal

et al. 2021

Food Science & Nutrition

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Spices are various plant parts that contain flavor and aroma compounds that in small amounts enhance the enjoyment value of foods.Nowadays, spices play a key role in modern food preparation as they contribute to salt reduction, reduce the utilization of artificial additives, impart natural color to foods, and act as natural antioxidants (Clemenson, 2019). Spices have been found to provide health benefits based on their bioactive ingredients. Diverse spices and herbs, namely anise, cinnamon, black cumin, curry, coriander, ginger, fenugreek, turmeric, garlic, mustard, pepper, and onion, are potentially involved in diabetes control (Sanlier & Gencerb, 2020).Spices are additives used in small quantities, owing to a high potential for microbial contamination as they are used in many food commodities. This possibility may be attributed to the existing various critical points (vulnerabilities) of microbial contamination during plant cultivation and product supply chain (Alegbeleye et al., 2018;Székács et al., 2018). Even though the spices have low water activity, they could be harbored by diverse pathogenic and spoilage microorganisms (Costa et al., 2020;Melo González et al., 2017). As dried herb products, they may be contaminated by different molds too.Due to the high tolerance to dehydration stress, Salmonella species can survive longer in dry products such as spices. The contamination can occur at several phases of the production including

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Section: Introductionmentioning

confidence: 99%

Antibiotic resistance pattern of the allochthonous bacteria isolated from commercially available spices

György

Laslo

Antal

et al. 2021

Food Science & Nutrition

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“…It is clear that the increased prevalence of drug-resistant bacteria has become a major public health problem [20,21]. In the current study, we studied 110 clinical isolates of Enterococcus (57 E. faecalis and 53 E. faecium).…”

Section: Discussionmentioning

confidence: 99%

Identification of two novel type II topoisomerase mutations in Enterococcus spp. isolated from a hospital in China

Peng

Wang

et al. 2021

ARCH BIOL SCI BELGRA

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Type II topoisomerases, including DNA gyrase (GyrA) and topoisomerase IV (ParC), contribute to fluoroquinolone resistance in Enterococcus spp. This study investigated the mutational status of the quinolone resistance-determining regions (QRDRs) of GyrA and ParC in the clinical isolates of enterococci from a hospital in Baotou, China. We analyzed 110 enterococcal isolates, including 57 Enterococcus faecalis and 53 Enterococcus faecalis faecium. The resistance rates of E. faecalis and E. faecium to ciprofloxacin were 63.16% and 84.91%, respectively. We found that 32 samples of E. faecalis and 42 of E. faecium had single or combined mutations in gyrA and/or parC, which were all resistant to ciprofloxacin. Only two ciprofloxacin-resistant E. faecalis isolates had no mutation. No mutations in gyrA and parC genes in all ciprofloxacin-susceptible isolates were found. Ciprofloxacin minimal inhibitory concentrations (MICs) in the mutation group were significantly higher than those of the nonmutation group, indicating that mutations in the QRDRs of gyrA and parC were correlated with MIC elevation. Two novel substitutions (GyrA Ser83Phe and ParC Ser80Leu) of E. faecalis were identified herein. Three-dimensional modeling revealed that these novel amino acid substitutions could disrupt the water/metal-ion bridge and decrease the interaction between the enzymes and ciprofloxacin. The data showed a diversity of mutation types in QRDRs of type II topoisomerases whose association with fluoroquinolone resistance in clinical isolates of enterococci warrants further investigation.

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“…The sources of their isolation were soil, hay, and manure. Strains' characterization and identification were performed previously [12,13]. The potential of 220 strains for their ability to tolerate heat stress (50, 60, 70 • C); tolerance to salinity by observing the growth on medium amended with various concentration of NaCl (5,7,13,15,17,20,22, and 25 % (w/v)); and drought tolerance obtained by adding 5, 10, 15, 20, 30, and 40% of poly(ethylene) glycol 6000 (PEG6000) in medium was tested.…”

Section: Bacterial Strain Selection and Characterization For Plant-gr...mentioning

confidence: 99%

“…Strains' characterization and identification were performed previously [12,13]. The potential of 220 strains for their ability to tolerate heat stress (50, 60, 70 • C); tolerance to salinity by observing the growth on medium amended with various concentration of NaCl (5,7,13,15,17,20,22, and 25 % (w/v)); and drought tolerance obtained by adding 5, 10, 15, 20, 30, and 40% of poly(ethylene) glycol 6000 (PEG6000) in medium was tested. From 220 natural bacterial isolates tested preliminarily, we selected six strains: Bacillus safensis SS-2.7, B. velezensis SS-6.4 and SS-8.2.2, B. thuringiensis SS-29.2, B. licheniformis SS-30.3, and B. paralicheniformis SS-37.5 that are able to grow at 50 • C in Luria-Bertani (LB) medium with 7% NaCl and in LB medium with 30% PEG6000.…”

Section: Bacterial Strain Selection and Characterization For Plant-gr...mentioning

confidence: 99%

Genotype-Dependent Antioxidative Response of Four Sweet Pepper Cultivars to Water Deficiency as Affected by Drought-Tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 Strains

et al. 2022

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We examined the effect of drought-tolerant Bacillus safensis SS-2.7 and B. thuringiensis SS-29.2 strains on the response of four (133, 274, California Wonder—CalW, and Matica) sweet pepper genotypes to water deficiency conditions. Pepper seeds were sown in pots with (treated) and without (control) bacterial strain inoculation. After four weeks of growth under controlled conditions and regular watering, drought was imposed by completely withholding watering for seven days. Under conditions of normal watering, genotype 274 showed better seedling establishment than genotype 133 and CalW, while the slowest was genotype Matica. Antioxidant enzyme activity under drought conditions was genotype and bacterial treatment-dependent. The best response to bacterial treatment in order to cope with severe drought was found in the CalW genotype, while in genotype 133, we determined even faster plant decay during water deficiency in treated seeds. Inoculated seeds of the Matica genotype did not show different antioxidant enzyme activity under normal and drought conditions. According to the obtained results, we concluded that under drought conditions, the most susceptible was genotype 274, moderate susceptibility was detected in genotype 133, and CalW and Matica were the most tolerant genotypes. Our study demonstrates (1) that drought-tolerant Bacillus strains showed a plant growth-promoting effect on some selected pepper genotypes; (2) that there were genotype-dependent antioxidant enzyme activities under drought conditions in response to treatment with a particular bacterial strain; and (3) that we could expect a genotype-dependent response during biostimulant application, especially under stress conditions.

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Identification and antibiotic resistance of Bacillus spp. isolates from natural samples

Cited by 8 publications

References 37 publications

Antibiotic resistance pattern of the allochthonous bacteria isolated from commercially available spices

Antibiotic resistance pattern of the allochthonous bacteria isolated from commercially available spices

Identification of two novel type II topoisomerase mutations in Enterococcus spp. isolated from a hospital in China

Genotype-Dependent Antioxidative Response of Four Sweet Pepper Cultivars to Water Deficiency as Affected by Drought-Tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 Strains

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