Identification and Antibiotic Resistance of Streptococcus agalactiae from Red Hybrid Tilapia (Oreochromis niloticus) in Local Wet Markets

Abstract

The existence of ethiology disease like Streptococcus agalactiae could be major problems in aquaculture field. In this study, investigations of red tilapia sold around local wet market were recorded. To detect the S. agalactiae in wet markets from disease potential and measure the antibiotic susceptibility test. Selected organs such as eyes, gills, heart and intestine were inoculated onto blood agar and incubated at 37°C for 24 h. Morphology of S. agalactiae were Gram-positive, translucent pin point colony, and has oxidase and catalase negative. Eight isolates of S. agalactiae were confirmed using PCR. The isolates samples were susceptible to imipenem (10 µg), gentamicin (10 µg), ciprofloxacin (5 µg) and nalidixic acid (30 µg). In contrast, they were resistance to ampicillin (10 µg), amoxicillin-clavulanic (20/10 µg), erythromycin (5 µg), neomycin (30 µg), norflaxacin (10 µg), streptomycin (10 µg) and trimethoprim-sulphamethoxazole (1.25/23.5 µg). Furthermore, the occurrence of resistant isolates is a matter of public health concern.

Streptococcus agalactiae Isolation and Characterization in Nile Tilapia (Oreochromis niloticus) with Histopathological Studies

“…The results obtained were in line with Delannoy et al ( 2013) who identified that Streptococcosis caused by S. agalactiae of cultured fish based on biochemical and serological characteristics. In addition, Auzureen et al (2016) stated that the Streptococcus agalactiae were a Grampositive, translucent pinpoint colony, and has oxidase and catalase-negative.The characterization of S. agalactiae have been screened and validated by VITEK 2 using biochemical methods. The obtained results were parallel to that obtained by (Laith et al, 2017).…”

Trial Evaluation of Protection and Immunogenicity of Piscine Bivalent Streptococcal Vaccine: From the Lab to the Farms

“…The outbreaks of streptococcosis have been related to environmental factors, including warm water temperatures, increased ammonia levels, and low dissolved oxygen [ 11 ]. S. agalactiae infections in tilapia have been reported in global aquaculture in different continents, especially Asia, including Thailand [ 17 , 18 , 19 , 20 , 21 , 22 ], Malaysia [ 23 , 24 , 25 , 26 , 27 ], Indonesia [ 28 , 29 ], Philippines [ 30 ], and China [ 31 , 32 , 33 , 34 ]. Importantly, the emergence of streptococcosis disease can take place anytime during tilapia farming, which may lead to the risk of disease distribution and loss of tilapia production if disease prevention and control are not well managed.…”