Identification and Antimicrobial Susceptibility of Coagulase Positive Staphylococci Isolated from Healthy Dogs and Dogs Suffering from Otitis Externa

Abstract

A total of 67 strains of coagulase positive staphylococci isolated from healthy dogs and dogs suffering from otitis externa were studied. Twenty-two isolates were from healthy dogs (five from hound dogs and 17 from companion dogs) and 45 from dogs suffering otitis externa (14 from hound dogs and 31 from companion dogs). Presumptive identification was attempted using the following tests: production of acetoin, anaerobic utilization of mannitol, acid production from mannitol, presence of beta-galactosidase, and growth on P agar supplemented with different concentrations of acriflavine. Susceptibility of staphylococci to 16 antibiotics was determined. Most effective antibiotics were imipenem, amoxycillin/clavulanic acid, ciprofloxacin, tobramycin, gentamicin and marbofloxacin. Penicillin, ampicillin and polymyxin B showed the lowest activity. There were no significant differences in antimicrobial susceptibility among isolates from healthy dogs and dogs suffering from otitis externa.

Molecular basis of resistance to macrolides and lincosamides among staphylococci and streptococci from various animal sources collected in the resistance monitoring program BfT-GermVet

“…Staphylococcus intermedius represented a major proportion of the canine and feline isolates identified in the Bft-GermVet study and the results were comparable with those of recent studies on S. intermedius isolated from dogs suffering from pyoderma or otitis externa [24][25][26][27]. One of these studies also investigated the genetic background of the observed macrolide-lincosamide-streptogramin B (MLS B ) resistance [27].…”

Microbiological Features of Staphylococcus schleiferi subsp. coagulans, Isolated from Dogs and Possible Misidentification with Other Canine Coagulase‐positive Staphylococci

“…The external auditory meatus of dogs is inhabited by various micro-organisms. Among them, different Staphylococcus species play a significant role (Junco and Barrasa, 2002). The most important pathogens are coagulase-positive staphylococci (CPS), especially S. intermedius (Hajek, 1976) and, to a lesser extent, S. aureus (Kloos and Schleifer, 1986) or S. schleiferi ssp.…”

Patógenos bacterianos en perros con otitis externa y sus perfiles de susceptibilidad a diferentes antimicrobianos

“…Os primeiros relatos com uso de tobramicina in vitro citavam 100% de eficiência do antimicrobiano contra cepas de Staphylococcus sp. Houve uma redução progressiva desse percentual (Junco & Barrasa, 2002) e em 2003 encontrava-se em torno de 82% . Acredita-se que esse percentual tenha reduzido significativamente, pois dados encontrados neste trabalho apresentaram 0 e 30% de eficiência para este antimicrobiano em relação às bactérias S. epidermidis e S. Pseudintermedius, respectivamente.…”

“…(Bornand, 1992, Leite, 2003, Silva, 2001, Junco & Barrasa, 2002. Tais resultados diferem dos apresentados no presente trabalho, onde a norfloxacina apresentou uma eficiência mediana (60 e 33%) para S. pseudintermedius e S. epidermidis.…”

“…Já para S. pseudintermedius, observou-se semelhança aos resultados do trabalho acima mencionado, com uma eficiência de 80% da ciprofloxacina sobre a bactéria. No tocante à enrofloxacina, observa-se uma redução discreta nos percentuais de suscetibilidade de 98% (Kiss et al, 1997, Silva, 2001) para 81% (Junco & Barrasa, 2002) devido à grande utilização desse fármaco de forma empírica. Estes resultados diferem drasticamente dos observados no presente trabalho, pois para ambas as bactérias os resultados obtidos quanto à eficiência do fármaco foram relativamente baixos (40% para a espécie pseudintermedius e 0% para epidermidis).…”