Identification and Antimicrobial Susceptibility of &lt;em&gt;Salmonella&lt;/em&gt; species Isolated from Washing and Rinsed Water of Broilers in Pluck Shops

Tuhin-Al-Ferdous,; Kabir, S. M. Lutful; Amin, M. M.; Hossain, Kamal

doi:10.19026/ijava.5.5569

Cited by 13 publications

(22 citation statements)

References 13 publications

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“…Two drops of oxidase reagent p -aminodimethylaniline oxalate were added to the surface of test organisms’ growth in oxidase test [ 21 ]. Nitrate broth (beef extract 3 g/L, peptone 5 g/L, potassium nitrate 5 g/L) [ 22 ] and MR-VP broth (peptone, dextrose and potassium phosphate) was prepared and incubated at 24-h at 37 °C for Methyl Red test and VP test [ 23 ]. The triple sugar iodine agar was prepared and after inoculation, incubated for about 24-h at 35 °C for TSI test [ 24 ].…”

Section: Main Textmentioning

confidence: 99%

Isolation and identification of halotolerant soil bacteria from coastal Patenga area

2017

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ObjectiveHalotolerant bacteria have multiple uses viz. fermentation with lesser sterility control and industrial production of bioplastics. Moreover, it may increase the crop productivity of coastal saline lands in Bangladesh by transferring the salt tolerant genes into the plants. The study focused on the isolation and identification of the halotolerant bacteria from three soil samples, collected from coastal Patenga area. The samples were inoculated in nutrient media containing a wide range of salt concentrations.ResultsAll the samples showed 2, 4 and 6% (w/v) salt tolerance. The isolates from Patenga soil (4, 6%) and beach soil (2%) showed catalase activity and all the isolates showed negative results for oxidase activity, indole production, lactose and motility. All the samples provided positive results for dextrose fermentation. Other tests provided mixed results. Based on the morphological characteristics, biochemical tests and ABIS software analysis the isolates fall within the Enterobacteriaceae, Clostridium and Corynebacterium, with a predominance of Vibrios. Overall the isolates can be considered as mild halotolerant, with the best growth observed at lower salinities and no halophilism detected. Among many possibilities, the genes responsible for the salt tolerant trait in these species can be identified, extracted and inserted into the crop plants to form a transgenic plant to result in higher yield for the rest of the year.

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Section: Main Textmentioning

confidence: 99%

Isolation and identification of halotolerant soil bacteria from coastal Patenga area

2017

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“…Production of cherry red reagent layer after introduction of Kovac’s reagent in MIU medium indicates Indole positive reaction [19-20]. …”

Section: Methodsmentioning

confidence: 99%

A Study on the Microbiological Status of Mineral Drinking Water

Aditi¹,

Rahman²,

Hossain³

2017

TOMICROJ

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Introduction:Water-borne diseases constitute a major health burden in Bangladesh. The objective of this study was to assess the overall quality of mineral water samples that obtained from different shops of Dhaka city.Material and Methods:To achieve the above-mentioned objective, methods of heterotrophic plate count (HPC) and total coliform count (TCC) were applied. Moreover, isolated colony from mineral water samples were characterized by using biochemical and antimicrobial susceptibility tests.Results:Different water samples showed different HPC ranged from 1.0×10 to 8.00×102. Antimicrobial sensitivity test of some selected bacteria viz S. intermedius, S. aureus, S. felis and S. Saccharolyticus were performed. It was observed that Staphylococcus spp. isolates were susceptible to erythromycin, tetracycline, norfloxacin and ciprofloxacin. Furthermore, a few Staphylococcus spp. isolates were intermediate resistant to penicillin and oxacillin. However, most of the Staphylococcus spp. isolates were resistant to cefixime.Conclusion:The results indicate that mineral water serves as a reservoir of various bacteria and that people in Dhaka city, who are the consumers of these water, might get diseases. This study emphasizes the need for elaborated microbiological examinations of mineral drinking water commonly used in Dhaka city.

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“…For indole production, 0.1 ml of chloroform was first added to each test tube and allowed for 15 min. Then 1ml of Kovac's reagent was added and then examined for red color development within 10 minutes as an indication of indole production [10].…”

Section: Motility Indole Urea (Miu) Testmentioning

confidence: 99%

“…Glucose phosphate broth was inoculated with the isolates and incubated for 2-5 days at 37°C. Then 5 drops of methyl red indicator (0.2%) was added and carefully observed for immediate development of a red color which indicates a positive test for acidic products [10].…”

Section: Methyl Red Testmentioning

confidence: 99%

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Antimicrobial Activity of Lactic Acid Bacteria from Kunun-Zaki on Selected Clinical Pathogens

Evurani

Anyim

Charity

et al. 2019

SAJRM

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Aims: To investigate the anti-microbial activity of lactic acid bacterial isolates from locally fermented Kunun-zaki beverage. Study Design: The study was designed to study the anti-microbial effect of lactic acid bacterial isolates obtained from Kunun-zaki beverage. Place and Duration of Study: Department of Applied Microbiology Enugu State University of Science and Technology, Agbani, June 2009 and January 2010. Methodology: Fresh Kunun drink was prepared from millet following standard modified procedures. Isolation of lactic acid bacteria from the beverage sample was carried out using MRS agar medium. Morphological and biochemical characterizations were performed to identify the organisms. Finally, the bacterial isolates were tested for antibacterial activity against clinical pathogens – Bacillus anthracis, Clostridium perfringens, Escherichia coli, Streptococcus faecalis, Micrococcus luteus, Klebsiella pneumonia, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis and Staphylococcus aureus obtained from Microbiology Department Nnamdi Azikiwe University Awka Nigeria. Results: Morphological and biochemical characteristics revealed the presence of Gram’s positive bacteria which were identified as Lactobacillus casei and Lactobacillus brevis. The antimicrobial activity of the isolates (L. casei and L. brevis) against various pathogenic strains recorded 90±0.002% inhibition. Only Micrococcus luteus was absolutely resistant to antimicrobial treatments with Lactobacillus casei and Lactobacillus brevis. Conclusion: The study concluded that Lactobacillus brevis and Lactobacillus casei from kunun-zaki drink possess strong antimicrobial activity against a wide range of clinical pathogens. Since lactic acid bacteria are known to possess many health benefits, some of the therapeutic effects reported from the consumption of the beverage may be attributed to these organisms.

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Identification and Antimicrobial Susceptibility of <em>Salmonella</em> species Isolated from Washing and Rinsed Water of Broilers in Pluck Shops

Cited by 13 publications

References 13 publications

Isolation and identification of halotolerant soil bacteria from coastal Patenga area

Isolation and identification of halotolerant soil bacteria from coastal Patenga area

A Study on the Microbiological Status of Mineral Drinking Water

Antimicrobial Activity of Lactic Acid Bacteria from Kunun-Zaki on Selected Clinical Pathogens

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