Identification and antioxidant activity test of bioactive compound produced from ethanol extract of temukunci (Boesenbergia rotunda)

Atun, Sri; Handayani, Sri; Frindryani, Luthfi Fitri

doi:10.1063/1.4995093

Cited by 7 publications

(5 citation statements)

References 6 publications

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“…Supplementary Figures S4-S7 show all the characterization spectra of the isolated crystal. All spectroscopic data are identical with those in the previous literature, including 1 H-NMR spectrum [54], GC-MS data [55], and UV-Vis spectrum [56]. A previous study reported that the isolation of pinostrobin using a non-chromatographic and precipitation technique yielded colorless crystals with 0.882% yield by precipitating with petroleum benzene during a slow cooling condition [57].…”

Section: Discussionsupporting

confidence: 69%

Boesenbergia rotunda and Its Pinostrobin for Atopic Dermatitis: Dual 5-Lipoxygenase and Cyclooxygenase-2 Inhibitor and Its Mechanistic Study through Steady-State Kinetics and Molecular Modeling

Liana,

Eurtivong,

Phanumartwiwath

2024

Antioxidants

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Human 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2) are potential targets for suppressing pruritic skin inflammation in atopic dermatitis (AD). In addition, Staphylococcus aureus colonization and oxidative stress worsen AD skin conditions. We aimed to investigate anti-inflammatory activity, using 5-LOX and COX-2 inhibitions, and the anti-staphylococcal, and antioxidant potentials of several medicinal plants bio-prospected from traditional medicine related to AD pathogenesis. Essential oils and hexane fractions were prepared and analyzed using gas chromatography–mass spectrometry. Boesenbergia rotunda hexane extract displayed anti-Staphylococcus aureus (MIC = 10 µg/mL) and antioxidant activities (IC50 = 557.97 and 2651.67 µg/mL against DPPH and NO radicals, respectively). A major flavonoid, pinostrobin, was further nonchromatographically isolated. Pinostrobin was shown to be a potent 5-LOX inhibitor (IC50 = 0.499 µM) compared to nordihydroguaiaretic acid (NDGA; IC50 = 5.020 µM) and betamethasone dipropionate (BD; IC50 = 2.077 µM) as the first-line of AD treatment. Additionally, pinostrobin inhibited COX-2 (IC50 = 285.67 µM), which was as effective as diclofenac sodium (IC50 = 290.35 µM) and BD (IC50 = 240.09 µM). This kinetic study and molecular modeling showed the mixed-type inhibition of NDGA and pinostrobin against 5-LOX. This study suggests that B. rotunda and its bioactive pinostrobin have promising properties for AD therapy.

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Section: Discussionsupporting

confidence: 69%

Boesenbergia rotunda and Its Pinostrobin for Atopic Dermatitis: Dual 5-Lipoxygenase and Cyclooxygenase-2 Inhibitor and Its Mechanistic Study through Steady-State Kinetics and Molecular Modeling

Liana,

Eurtivong,

Phanumartwiwath

2024

Antioxidants

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“…This compound is a major component in the rhizome of B. rotunda plant, and previously this compound has been obtained as a crystalline solid in the ethanol extract of rhizome B. rotunda. 15 The structures of isolated compound-1, -2, and -3 are show in Figure 1. Antioxidant activity assay for the samples were indentified using DPPH method.…”

Section: Resultsmentioning

confidence: 99%

Potential Bioactive Compounds Isolated from Boesenbergia rotunda as Antioxidant and Antimicrobial Agents

Atun¹,

Handayani²,

Rakhmawati³

2018

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Objective: This study was conducted to identify some bioactive compounds of Boesenbergia rotunda rhizome and to test as antioxidant and antimicrobial agents. Methods: The milled dried rhizome of B. rotunda (5kg) was extracted exhaustively with ethanol. The ethanol extract was partitioned three times by n-hexane, chloroform, and ethyl acetate respectively. Each fraction was fractionated by vacuum liquid chromatography (VLC) and then purified by column chromatography gravitation. Structural identification of all pure compounds were elucidated based on spectroscopic methods (UV, IR, and NMR).The antioxidant activity was tested by 2,2-diphenyl-1-picrylhydrazyl (DPPH). Antimicrobial character was screened for activities againts pathogenic bacteria i.e. Escherichia coli ATCC-11229, Staphylococcus aureus ATCC-25923, Staphylococcus epidermidis FNCC-0048, and Streptococcus mutans by the disk-diffusion method. The assay was done in triplicate, and chloramphenicol was used as the positive control. Results and Discussion: From ethanol extract of B. rotunda three known compounds of flavanones, namely 2',4'-dihydroxy-6-methoxychalcone (compound-1), 5-hydroxy-7methoxyflavanone (compound-2), and 5,7-dihydroxyflavanone (compound-3) were isolated. The ethanol extract of B. rotunda and the three isolated compounds-1,-2, and-3 showed an antioxidant activity with the IC value of 92.64; 46.66; 62.84, and 62.66 µg/mL, respectively. The zone of inhibition of extract and the three isolated compounds showed moderate activity against Escherichia coli ATCC-11229, Staphylococcus aureus ATCC-25923, Staphylococcus epidermidis FNCC-0048, and Streptococcus mutans.The maximum zone of inhibition was 13.20±0.76 mm at the maximum concentration used (500 µg/mL) against Escherichia coli ATCC-11229, and the minimum inhibitory concentration(MIC) for each bacteria was found to be 0.5µg/mL. Conclusion: The result of the study suggests that B. rotunda rhizome contains potential bioactive compounds which could be suitable for antioxidant and the treatment of various infections caused by Escherichia coli ATCC-11229, Staphylococcus aureus ATCC-25923, Staphylococcus epidermidis FNCC-0048, and Streptococcus mutans.

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“…Pinostrobin (PN) (Fig. 1d) is one of the most flavones found in fingerroot (Boesenbergia rotunda) and honey which is commonly potential as an antioxidant and contribute against several diseases (Fahey and Stephenson, 2002;Chahyadi et al, 2014;Patel et al, 2016;Atun et al, 2017;Jadaun et al, 2019). Even though there are few numbers of reports regarding pinostrobin, some are reported to have roles to some cancers, such as in breast cancer by binding with estrogen receptor and fibrosarcoma by upregulating the expression of p53 and decreasing activation of VEGF angiogenesis (Le Bail et al, 2000;Parwata et al, 2015).…”

Section: Pinostrobinmentioning

confidence: 99%

The activity of bioactive compounds against several diseases by modulating autophagy

Qomaladewi¹,

Cho²

2021

crbb

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Several bioactive compounds derived from Indonesia plants, such as xanthorrhizol, gingerol, and pinostrobin are the major compound in a certain plant. They have some pharmacological activities against several diseases, such as inflammation, tumor, and cancer, and modulate other mechanisms. One of them is one of programmed cell death called autophagy. The regulation involves several pathways on upstream and downstream of the autophagy mechanism and affects its activity. Each of the compound has different site of target when it comes to different case of diseases. Here we explained how the bioactive compounds regulate autophagy against several diseases.

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Identification and antioxidant activity test of bioactive compound produced from ethanol extract of temukunci (Boesenbergia rotunda)

Cited by 7 publications

References 6 publications

Boesenbergia rotunda and Its Pinostrobin for Atopic Dermatitis: Dual 5-Lipoxygenase and Cyclooxygenase-2 Inhibitor and Its Mechanistic Study through Steady-State Kinetics and Molecular Modeling

Boesenbergia rotunda and Its Pinostrobin for Atopic Dermatitis: Dual 5-Lipoxygenase and Cyclooxygenase-2 Inhibitor and Its Mechanistic Study through Steady-State Kinetics and Molecular Modeling

Potential Bioactive Compounds Isolated from Boesenbergia rotunda as Antioxidant and Antimicrobial Agents

The activity of bioactive compounds against several diseases by modulating autophagy

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