Identification and assessment of genetic relationships in three Chlorophytum species and two high yielding genotypes of C. borivilianum through RAPD markers

Abstract

Conservation of identified germplasm is an important component for efficient and effective management of plant genetic resources. Since Chlorophytum species are important medicinal plants, studies were carried out for identification and establish genetic relationships in three species of Chlorophytum and two high yielding genotypes of Chlorophtum borivilianum using RAPD markers. Out of one hundred primers tested, 47 decamers amplified a total of 454 distinct bands ranging from 0.25-3.0 kbp to identify and to evaluate genetic relationships between and among three species of Chlorophytum and two genotypes of Chlorophtum borivilianum. The cluster analysis indicated that three species of Chlorophytum and two genotypes (NRCCB-1 and NRCCB-2) of C. borivilianum formed two major clusters. The first major cluster constituted C. arundinaceum and C. tuberosum, and the second major cluster composed of two subclusters; the first subcluster represented NRCB-1 and NRCB-2 where as the second subcluster represented C. borivilianum. Thus, the RAPD markers have the potential for identification and characterization of genetic relatedness among the species and genotypes. C. borivilianum along with two genotypes also showed similar banding patterns which could be chosen as candidate markers for differentiating the other two species such as C. arundinaceum and C. tuberosum. This would helpful for breeding programmes and provides an important input in conservation biology.

Genetic variation of horse chestnut and red horse chestnut and trees susceptibility to Erysiphe flexuosa and Cameraria ohridella

“…In this study we assessed the genetic diversity within and between two Aesculus species using well known RAPD method and rarely applied SRAP method. RAPD method is useful in detection of DNA polymorphisms without prior knowledge of the genome sequence and was widely applied to the plant genome study (Irzykowska et al 2002;Nanda et al 2004;Sesli & Yegenoglu 2009;Samantaray et al 2011). In our study RAPD analyses also were efficient and from 6 to 9 polymorphic DNA fragments per primer were amplified.…”