Identification and biochemical characterization of macrophage migration inhibitory factor-2 (MIF-2) homologue of human lymphatic filarial parasite, Wuchereria bancrofti

Chauhan, Nikhil; Sharma, Rohit; Hoti, S.L.

doi:10.1016/j.actatropica.2014.10.009

Cited by 17 publications

(11 citation statements)

References 48 publications

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“…Another published phylogenetic reconstruction of experimentally studied MIFs noted that they may have evolved from an ancient defense molecule; in parasites MIF-like proteins appear to function as virulence factors ( 23 ). A MIF-like protein has been characterized biochemically from the human parasite Wuchereria bancrofti (wbMIF) and shows a low level of tautomerase activity ( 54 ). This latter analysis is the only known attempt to assign function to non-mammalian MIFs.…”

Section: Resultsmentioning

confidence: 99%

A global view of structure–function relationships in the tautomerase superfamily

Davidson

Baas²,

Akiva

et al. 2018

Journal of Biological Chemistry

163

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The tautomerase superfamily (TSF) consists of more than 11,000 nonredundant sequences present throughout the biosphere. Characterized members have attracted much attention because of the unusual and key catalytic role of an N-terminal proline. These few characterized members catalyze a diverse range of chemical reactions, but the full scale of their chemical capabilities and biological functions remains unknown. To gain new insight into TSF structure–function relationships, we performed a global analysis of similarities across the entire superfamily and computed a sequence similarity network to guide classification into distinct subgroups. Our results indicate that TSF members are found in all domains of life, with most being present in bacteria. The eukaryotic members of the cis-3-chloroacrylic acid dehalogenase subgroup are limited to fungal species, whereas the macrophage migration inhibitory factor subgroup has wide eukaryotic representation (including mammals). Unexpectedly, we found that 346 TSF sequences lack Pro-1, of which 85% are present in the malonate semialdehyde decarboxylase subgroup. The computed network also enabled the identification of similarity paths, namely sequences that link functionally diverse subgroups and exhibit transitional structural features that may help explain reaction divergence. A structure-guided comparison of these linker proteins identified conserved transitions between them, and kinetic analysis paralleled these observations. Phylogenetic reconstruction of the linker set was consistent with these findings. Our results also suggest that contemporary TSF members may have evolved from a short 4-oxalocrotonate tautomerase–like ancestor followed by gene duplication and fusion. Our new linker-guided strategy can be used to enrich the discovery of sequence/structure/function transitions in other enzyme superfamilies.

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Section: Resultsmentioning

confidence: 99%

A global view of structure–function relationships in the tautomerase superfamily

Davidson

Baas²,

Akiva

et al. 2018

Journal of Biological Chemistry

163

View full text Add to dashboard Cite

show abstract

“…Presumably, Bma-MIF-2 lacks the oxidoreductase activity unlike MIF-1, as it does not have CXXC motif (Zang et al, 2002). Wba-MIF-2 also lacks the CXXC motif but, we found significant oxidoreductase activity of this homolog in the insulin reduction assay (Chauhan et al, 2015), plausibly because of the presence of other vicinal cysteine residues. Two conserved vicinal cysteine residues of MIF are reported to play a role in the oxidoreductase activity, because of oxidation and reduction properties of SH-groups.…”

Section: Introductionmentioning

confidence: 82%

“…Earlier, we have reported the characterization of MIF-1 (Wba-mif-1) and MIF-2 (Wba-mif-2) from Wuchereria bancrofti (Sharma et al, 2012;Chauhan et al, 2015), the major causal organism of human lymphatic filariasis. The alignment of derived amino acid sequences of Wba-MIF-2 with Wba-MIF-1 showed 44% homology.…”

Section: Introductionmentioning

confidence: 99%

Role of cysteine-58 and cysteine-95 residues in the thiol di-sulfide oxidoreductase activity of Macrophage Migration Inhibitory Factor-2 of Wuchereria bancrofti

Chauhan

Hoti

2016

Acta Tropica

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“…Cytokines are commonly mimicked by parasitic nematodes in their efforts to modulate the host immune system. One such ortholog is Macrophage Inhibitory Factor (MIF), which is produced by many nematodes including B. malayi, Anisakis simplex, Wuchereria bancrofti, and H. contortus (Bennuru et al, 2009;Chauhan et al, 2015;Wang et al, 2017b;Harischandra et al, 2018). Murine MIF is important for alternative activation of macrophages, promotes the Th2 response during nematode infection, and is required for optimal worm clearance (Filbey et al, 2019).…”

Section: Proteinsmentioning

confidence: 99%

The Two Faces of Nematode Infection: Virulence and Immunomodulatory Molecules From Nematode Parasites of Mammals, Insects and Plants

2020

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Helminths stage a powerful infection that allows the parasite to damage host tissue through migration and feeding while simultaneously evading the host immune system. This feat is accomplished in part through the release of a diverse set of molecules that contribute to pathogenicity and immune suppression. Many of these molecules have been characterized in terms of their ability to influence the infectious capabilities of helminths across the tree of life. These include nematodes that infect insects, known as entomopathogenic nematodes (EPN) and plants with applications in agriculture and medicine. In this review we will first discuss the nematode virulence factors, which aid parasite colonization or tissue invasion, and cause many of the negative symptoms associated with infection. These include enzymes involved in detoxification, factors essential for parasite development and growth, and highly immunogenic ES proteins. We also explore how these parasites use several classes of molecules (proteins, carbohydrates, and nucleic acids) to evade the host’s immune defenses. For example, helminths release immunomodulatory molecules in extracellular vesicles that may be protective in allergy and inflammatory disease. Collectively, these nematode-derived molecules allow parasites to persist for months or even years in a host, avoiding being killed or expelled by the immune system. Here, we evaluate these molecules, for their individual and combined potential as vaccine candidates, targets for anthelminthic drugs, and therapeutics for allergy and inflammatory disease. Last, we evaluate shared virulence and immunomodulatory mechanisms between mammalian and non-mammalian plant parasitic nematodes and EPNs, and discuss the utility of EPNs as a cost-effective model for studying nematode-derived molecules. Better knowledge of the virulence and immunomodulatory molecules from both entomopathogenic nematodes and soil-based helminths will allow for their use as beneficial agents in fighting disease and pests, divorced from their pathogenic consequences.

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Identification and biochemical characterization of macrophage migration inhibitory factor-2 (MIF-2) homologue of human lymphatic filarial parasite, Wuchereria bancrofti

Cited by 17 publications

References 48 publications

A global view of structure–function relationships in the tautomerase superfamily

A global view of structure–function relationships in the tautomerase superfamily

Role of cysteine-58 and cysteine-95 residues in the thiol di-sulfide oxidoreductase activity of Macrophage Migration Inhibitory Factor-2 of Wuchereria bancrofti

The Two Faces of Nematode Infection: Virulence and Immunomodulatory Molecules From Nematode Parasites of Mammals, Insects and Plants

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