Identification and cDNA cloning for S-RNases in self-incompatible Japanese plum (Prunus salicina Lindl. cv. Sordum).

Yamane, Hisayo; Tao, Ryutaro; Sugiura, Akira

doi:10.5511/plantbiotechnology.16.389

Cited by 39 publications

(41 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because of the presence of at least two multi-allelic genes, the term "haplotype" has been used to describe variants of the S-locus and the term "allele" to describe variants of a given polymorphic gene at the S-locus (McCubbin and Kao 2000). In the Solanaceae and the Rosaceae, the gene controlling the pistil's SI response is a ribonuclease (S-RNase) which is expressed only in the pistil (McClure et al 1989;Sassa et al 1992;Lee et al 1994;Murfett et al 1994;Broothaerts et al 1995;Boškovic and Tobutt 1996;Ishimizu et al 1996;Sassa et al 1996;Tomimoto et al 1996;Tao et al 1997Tao et al , 1999Burgos et al 1998;Ushijima et al 1998;Yamane et al 1999). A second gene that is hypothesized to be expressed specifically in the pollen has yet to be determined from any GSI species.…”

Section: Introductionmentioning

confidence: 99%

Self-compatibility and incompatibility in tetraploid sour cherry ( Prunus cerasus L.)

Hauck

Yamane

Tao

et al. 2002

Sexual Plant Reproduction

Self Cite

View full text Add to dashboard Cite

Gametophytic self-incompatibility (GSI) typically "breaks down" due to polyploidy in many Solanaceous species, resulting in self-compatible (SC) tetraploid individuals. However, sour cherry (Prunus cerasus L.), a tetraploid species resulting from hybridization of the diploid sweet cherry (P. avium L.) and the tetraploid ground cherry (P. fruticosa Pall.), is an exception, consisting of both self-incompatible (SI) and SC individuals. Since sweet cherry exhibits GSI with 13 S-ribonucleases (S-RNases) identified as the stylar S-locus product, the objectives were to compare sweet and sour cherry S-allele function, S-RNase sequences and linkage map location as initial steps towards understanding the genetic basis of SI and SC in sour cherry. S-RNases from two sour cherry cultivars that were the parents of a linkage mapping population were cloned and sequenced. The sequences of two S-RNases were identical to those of sweet cherry S-RNases, whereas three other S-RNases had unique sequences. One of the S-RNases mapped to the Prunus linkage group 6, similar to its location in sweet cherry and almond, whereas two other S-RNases were linked to each other but were unlinked to any other markers. Interspecific crosses between sweet and sour cherry demonstrated that GSI exists in sour cherry and that the recognition of common S-alleles has been maintained in spite of polyploidization. It is hypothesized that self-compatibility in sour cherry is caused by the existence of non-functional S-RNases and pollen S-genes that may have arisen from natural mutations.

show abstract

Section: Introductionmentioning

confidence: 99%

Self-compatibility and incompatibility in tetraploid sour cherry ( Prunus cerasus L.)

Hauck

Yamane

Tao

et al. 2002

Sexual Plant Reproduction

Self Cite

View full text Add to dashboard Cite

show abstract

“…is a commercially important fruit crop, which exhibits GSI. Yamane et al (1999) have demonstrated the presence of S-RNases in Japanese plum and have cloned two of their cDNAs. S-RNase in Japanese plum is a basic glycoprotein with Mr of about 30 kDa containing two active sites for T2/S type RNases, five conserved regions and one hypervariable region and is expressed exclusively in the pistil.…”

Section: Introductionmentioning

confidence: 99%

Identification of S-haplotype-specific F-box gene in Japanese plum (Prunus salicina Lindl.)

et al. 2006

View full text Add to dashboard Cite

Self-incompatibility has been studied extensively at the molecular level in Solanaceae, Rosaceae and Scrophulariaceae, all of which exhibit gametophytic self-incompatibility controlled by a single polymorphic locus containing at least two linked genes, i.e., the S-RNase gene and the pollen-expressed SFB/SLF (S-haplotype-specific F-box/S-locus F-box) gene. However, the SFB gene in Japanese plum (Prunus salicina Lindl.) has not yet been identified. We determined eight novel sequences homologous to the SFB genes of other Prunus species and named these sequences PsSFB. The gene structure of the SFB genes and the characteristic domains in deduced amino acid sequences were conserved. Three sequences from 410 to 2,800 bp of the intergenic region between the PsSFB sequences and the S-RNase alleles were obtained. The eight identified PsSFB sequences showed S-haplotypespecific polymorphism, with 74-83% amino acid identity. These alleles were exclusively expressed in the pollen. These results suggest that the PsSFB alleles are the pollen S-determinants of GSI in Japanese plum.

show abstract

“…et Zucc.) that belongs to the Rosaceae exhibits S-RNase-based gametophytic self-incompatibility Yaegaki et al 2001), as do most other Prunus fruit tree species (Tao et al 1997Ushijima et al 1998;Yamane et al 1999Yamane et al , 2001. Although both self-incompatible and selfcompatible cultivars are grown commercially in Japan, self-compatible cultivars have a horticultural advantage over self-incompatible cultivars because no cross-pollinizer is required.…”

Section: Introductionmentioning

confidence: 99%

Inheritance of S f-RNase in Japanese apricot (Prunus mume) and its relation to self-compatibility

et al. 2002

View full text Add to dashboard Cite

Self-compatible cultivars of Japanese apricot ( Prunus mume Shieb. et Zucc.), a tree species that normally shows S-RNase-based self-incompatiblity, have a horticultural advantage over self-incompatible cultivars. Inheritance of self-compatibility and a common S(f)-RNase allele that is observed in self-compatible cultivars was investigated using progenies from controlled crosses. Total DNAs were isolated from the parents and progenies of seven crosses that included at least one self-compatible cultivar as a parent. These DNAs were PCR-amplified with the Pru-C2 and PCE-R primer pair to determine S-haplotypes of the parents and progenies. A novel S-haplotype, S(8), was found. In all crosses examined, the S(f)-RNase gene was inherited from either the seed or pollen parent as a pistil S-allele in a non-functional S-haplotype. Self-compatibility of about 20 trees each from reciprocal crosses of 'Benisashi ( S(7) S(f))' and 'Shinpeidayu ( S(3) S(f))', and 26 selections from 16 different crosses was tested by pollination and pollen-tube growth studies. Cosegregation of the S(f)-RNase allele and self-compatibility was confirmed with all but selection 1K0-26 ( S(3) S(7)). Selection 1K0-26 ( S(3) S(7)) that originated from 'Benisashi ( S(7) S(f))' x 'Koshinoume ( S(3) S(f))' appeared to be self-compatible even without the S(f)-RNase allele. The possible role of pollen- S, a presumably existing pollen component of gametophytic self-incompatibility, is discussed.

show abstract

Identification and cDNA cloning for S-RNases in self-incompatible Japanese plum (Prunus salicina Lindl. cv. Sordum).

Cited by 39 publications

References 29 publications

Self-compatibility and incompatibility in tetraploid sour cherry ( Prunus cerasus L.)

Self-compatibility and incompatibility in tetraploid sour cherry ( Prunus cerasus L.)

Identification of S-haplotype-specific F-box gene in Japanese plum (Prunus salicina Lindl.)

Inheritance of S f-RNase in Japanese apricot (Prunus mume) and its relation to self-compatibility

Contact Info

Product

Resources

About