Identification and Characterisation of a Novel Protein FIP-sch3 from Stachybotrys chartarum

Abstract

In this study, a novel FIP named FIP-sch3 has been identified and characterised. FIP-sch3 was identified in the ascomycete Stachybotrys chartarum, making it the second FIP to be identified outside the order of Basidiomycota. Recombinant FIP-sch3 (rFIP-shc3) was produced in Escherichia coli and purified using GST-affinity magnetic beads. The bioactive characteristics of FIP-sch3 were compared to those of well-known FIPs LZ-8 from Ganoderma lucidum and FIP-fve from Flammulina velutipes, which were produced and purified using the same method. The purified rFIP-sch3 exhibited a broad spectrum of anti-tumour activity in several types of tumour cells but had no cytotoxicity in normal human embryonic kidney 293 cells. Assays that were implemented to study these properties indicated that rFIP-sch3 significantly suppressed cell proliferation, induced apoptosis and inhibited cell migration in human lung adenocarcinoma A549 cells. The anti-tumour effects of rFIP-sch3 in A549 cells were comparable to those of rLZ-8, but they were significantly greater than those of rFIP-fve. Molecular assays that were built on real-time PCR further revealed potential mechanisms related to apoptosis and migration and that underlie phenotypic effects. These results indicate that FIP-shc3 has a unique anti-tumour bioactive profile, as do other FIPs, which provide a foundation for further studies on anti-tumour mechanisms. Importantly, this study also had convenient access to FIP-sch3 with potential human therapeutic applications.

Recombinant Expression and Bioactivity Comparison of Four Typical Fungal Immunomodulatory Proteins from Three Main Ganoderma Species

“…Additionally, Ganoderma FIPs showed similar but weaker cytotoxic activities by contrast with some other FIPs, for example, FIP-lrh from Lignosus rhinocerotis , exhibited stronger cytotoxicity to A549, Hela and MCF-7 cancer cells, with IC 50 values ranging from 5.07 to 8.94 μg/mL [46]. And the IC 50 value of another FIP, FIP-sch3 ( Stachybotrys chartarum ), was 10.80 μg/mL [29]. Besides, the cytotoxicity of rFIP-gaps towards A549 and Hela cells in current study was accordant with those in previous reports with very similar IC 50 values [35].…”

Identification of a Novel Anti-cancer Protein, FIP-bbo, from Botryobasidium botryosum and Protein Structure Analysis using Molecular Dynamic Simulation

“…Although both rLZ-8 and FIP-fve prevented lung cancer cell proliferation via the increase G1 arrest, their effect strengths are different. The anti-cancer effect of LZ-8 is stronger than that of the FIP-fve 8–10 .…”

Stachybotrys chartarum—A Hidden Treasure: Secondary Metabolites, Bioactivities, and Biotechnological Relevance

“…rFIP-shc3 (recombinant FIP-sch3) exhibited a potent anti-tumor potential versus MCF-7, H520, A549, HeLa, and HepG2 but had no effect in 293 (normal human embryonic kidney) cells. It significantly inhibited cell migration and proliferation via the induced apoptosis in A549 cells [ 135 ]. Li et al stated that the solid rice culture EtOAc extract of S. chartarum , harboring Niphates recondite sponge, displayed noticeable cytotoxic capacity (IC 50 <10 mg/mL) versus a panel of tumor cell lines (e.g., BGC-823, HCT-116 NCI-H1650, A2780, and HepG2) [ 92 ].…”