Identification and Characterisation of Endophytic Bacteria from Coconut (Cocos nucifera) Tissue Culture

Salo, Elv Nhiel; Novero, Annabelle U.

doi:10.21315/tlsr2020.31.1.4

Cited by 13 publications

(5 citation statements)

References 32 publications

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“…Strain CTXW 7-6-2 was identified as a B. subtilis strain via morphological observation and combined analysis of 16S rRNA, gyrA, rpoB , and purH . Additionally, strain CTXW 7-6-2 produced a light-red pigment, which was consistent with an unknown B. subtilis strain isolated from coconut tissue culture ( Salo and Novero, 2020 ). Strain CTXW 7-6-2 inhibited pathogenic fungi associated with diseases that infect kiwifruit, tobacco, plums, and Chinese ground orchids, and adversely affected the hyphae of the pathogenic fungi B. dothidea , Phomopsis sp., and A. alternata , which cause soft rot.…”

Section: Discussionsupporting

confidence: 78%

Biocontrol potential of Bacillus subtilis CTXW 7-6-2 against kiwifruit soft rot pathogens revealed by whole-genome sequencing and biochemical characterisation

Chen

Zhang

et al. 2022

Front. Microbiol.

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Soft rot causes significant economic losses in the kiwifruit industry. This study isolated strain CTXW 7-6-2 from healthy kiwifruit tissue; this was a gram-positive bacterium that produced the red pigment pulcherrimin. The phylogenetic tree based on 16S ribosomal RNA, gyrA, rpoB, and purH gene sequences identified CTXW 7-6-2 as a strain of Bacillus subtilis. CTXW 7-6-2 inhibited hyphal growth of pathogenic fungi that cause kiwifruit soft rot, namely, Botryosphaeria dothidea, Phomopsis sp., and Alternaria alternata, by 81.76, 69.80, and 32.03%, respectively. CTXW 7-6-2 caused the hyphal surface to become swollen and deformed. Volatile compounds (VOC) produced by the strain inhibited the growth of A. alternata and Phomopsis sp. by 65.74 and 54.78%, respectively. Whole-genome sequencing revealed that CTXW 7-6-2 possessed a single circular chromosome of 4,221,676 bp that contained 4,428 protein-coding genes, with a guanine and cytosine (GC) content of 43.41%. Gene functions were annotated using the National Center for Biotechnology Information (NCBI) non-redundant protein, Swiss-Prot, Kyoto Encyclopedia of Genes and Genomes, Clusters of Orthologous Groups of proteins, Gene Ontology, Pathogen–Host Interactions, Carbohydrate-Active enZYmes, and Rapid Annotations using Subsystem Technology databases, revealing non-ribosomal pathways associated with antifungal mechanisms, biofilm formation, chemotactic motility, VOC 3-hydroxy-2-butanone, cell wall-associated enzymes, and synthesis of various secondary metabolites. antiSMASH analysis predicted that CTXW 7-6-2 can produce the active substances bacillaene, bacillibactin, subtilosin A, bacilysin, and luminmide and has four gene clusters of unknown function. Quantitative real-time PCR (qRT-PCR) analysis verified that yvmC and cypX, key genes involved in the production of pulcherrimin, were highly expressed in CTXW 7-6-2. This study elucidates the mechanism by which B. subtilis strain CTXW 7-6-2 inhibits pathogenic fungi that cause kiwifruit soft rot, suggesting the benefit of further studying its antifungal active substances.

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Section: Discussionsupporting

confidence: 78%

Biocontrol potential of Bacillus subtilis CTXW 7-6-2 against kiwifruit soft rot pathogens revealed by whole-genome sequencing and biochemical characterisation

Chen

Zhang

et al. 2022

Front. Microbiol.

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“…Most of the soil isolates were also rod-shaped, gram-positive, catalase-producing, starch-hydrolyzing, oxidase and phosphate-producing isolates. These characteristics in this present study are not sufficient to identify the isolates to the genus level [ 53 ].…”

Section: Discussionmentioning

confidence: 91%

In vitro antibacterial activities and molecular characterization of bacterial species isolated from farmlands against selected pathogens

Onajobi

Idowu

Adeyemi

et al. 2020

Biotechnology Reports

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Highlights Screening bacterial isolates for antagonistic potential. Molecular methods for identification of isolates. Six potent antibacterial isolates against the test pathogenic species. Pseudomonas was susceptible to antagonizing strains. Necessity for new effective antibacterial formulation.

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“…These isolates dominant from genus of Bacillus. Salo & Novero, (2020) reported that plants of same type or species have endophytic bacteria that are not always same. Research (Giyanto & Anwar Arifin, 2019) reported that isolates of endophytic bacteria isolated from cocoa plants could spur growth of cocoa plants.…”

Section: Root Length (Cm)mentioning

confidence: 99%

Invasion of Endophytic Bacteria by Using Microencapsulation Technology as Stimulant in Cocoa Plants (Theobroma cacao L)

Prayoga,

Warsito,

Amrul

2023

JPBN

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Fertilization is an important process in the growth of cocoa (Theobroma cacao L). Fertilizing cocoa usually used inorganic fertilizers. Using of inorganic fertilizers continuously could degrading soil fertility. One of the efforts to replace inorganic fertilizers was using endophytic bacteria as biofertilizers. Some endophytic bacteria are abundant in healthy plant tissues. The aim of this research was to obtain endophytic auxin-producing bacteria in cocoa plants and determine the effect of seed immersion and microcapsules addition of endophytic bacteria on cocoa plant growth. The research design used was Complete Randomized Design (CRD), 16 treatments and 3 replications. The first factor was immersion of cocoa seed using endophytic bacteria suspension consisting of S0= 0 hours; S1= 5 hours; S2= 6 hours and S3= 7 hours and the second factor was microcapsules addition consisting of B0= 0 gr; B1= 5 gr; B2= 10 gr; B3= 15 gr. Isolation from the roots and stems of cocoa obtained 5 isolates endophyte bacteria. Auxin assays showed that five isolates were able to produce auxin. Observations on plant height showed the best treatment was treatment B1 (32.49 cm). Observation of total leaves showed S3 treatment was the highest data (11.83 strands). Observation rod diameter parameter, highest data was S3 treatment (4.01 mm). For leaf area parameter, highest value was B1 treatment (66.64 cm2). For wet weight parameter, highest data was S3 treatment (18.41 g). Root length parameter, highest data was B3 treatment (15.78 cm). Test results showed that the application of suspension and microcapsules of endophytic bacteria significantly increased growth of cocoa.

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Identification and Characterisation of Endophytic Bacteria from Coconut (Cocos nucifera) Tissue Culture

Abstract: Highlights • Five bacterial species were isolated and identified from coconut tissues. • Morphological, biochemical and molecular analyses revealed that four of the isolates were different strains of Bacillus subtillis. • The fifth isolate was identified as Pantoea dispersa.

Cited by 13 publications

References 32 publications

Biocontrol potential of Bacillus subtilis CTXW 7-6-2 against kiwifruit soft rot pathogens revealed by whole-genome sequencing and biochemical characterisation

Biocontrol potential of Bacillus subtilis CTXW 7-6-2 against kiwifruit soft rot pathogens revealed by whole-genome sequencing and biochemical characterisation

In vitro antibacterial activities and molecular characterization of bacterial species isolated from farmlands against selected pathogens

Invasion of Endophytic Bacteria by Using Microencapsulation Technology as Stimulant in Cocoa Plants (Theobroma cacao L)

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