Identification and characterisation of hyaluronate lyase from Streptococcus suis

Allen, Andrew G.; Lindsay, Heather; Seilly, David J.; Bolitho, Steven; Peters, Sarah; Maskell, Duncan J.

doi:10.1016/j.micpath.2004.02.006

Cited by 30 publications

(19 citation statements)

References 22 publications

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“…Naming (SSU numbering) is derived from the annotated genome sequence of P1/7 [7]. Presence of 25 described putative virulence factors was studied: muramidase released protein ( mrp ), and extracullar factor ( epf ) [13], suilysin ( sly ) [20], sortases ( srtA , srtBCD , srtF ) [34], surface antigen one ( sao ) [42], hyaluronidase ( hylA ) [17,43], opacity factor ( ofs ) [37], fibronectin binding protein ( fbps ) [44], arginin deiminase ( arcA ) [45], glyceraldehyde-3-phosphate dehydrogenase ( gapdh ) [46], regulator of virulence ( revS ) [35,47], enolase ( eno ) [48], glutamine synthetase ( glnA ) [49], igA1 protease [36], inosine 5-monophosphate dehydrogenase ( impdh ) [50], dipeptidyl peptidase IV ( dppIV ) [51], ferrous iron transporter ( feoB ) [52], subtilisin like serine protease ( sspA ) [53], amylopullulanase ( apuA ) [54], ferric uptake regulator ( fur ), and adhesion competence repressor ( adcR ) [55]. * hylA is present as pseudogene in P1/7 and does not have a SSU-number.…”

Section: Resultsmentioning

confidence: 99%

Genetic diversity of Streptococcus suis isolates as determined by comparative genome hybridization

et al. 2011

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BackgroundStreptococcus suis is a zoonotic pathogen that causes infections in young piglets. S. suis is a heterogeneous species. Thirty-three different capsular serotypes have been described, that differ in virulence between as well as within serotypes.ResultsIn this study, the correlation between gene content, serotype, phenotype and virulence among 55 S. suis strains was studied using Comparative Genome Hybridization (CGH). Clustering of CGH data divided S. suis isolates into two clusters, A and B. Cluster A isolates could be discriminated from cluster B isolates based on the protein expression of extracellular factor (EF). Cluster A contained serotype 1 and 2 isolates that were correlated with virulence. Cluster B mainly contained serotype 7 and 9 isolates. Genetic similarity was observed between serotype 7 and serotype 2 isolates that do not express muramidase released protein (MRP) and EF (MRP-EF-), suggesting these isolates originated from a common founder. Profiles of 25 putative virulence-associated genes of S. suis were determined among the 55 isolates. Presence of all 25 genes was shown for cluster A isolates, whereas cluster B isolates lacked one or more putative virulence genes. Divergence of S. suis isolates was further studied based on the presence of 39 regions of difference. Conservation of genes was evaluated by the definition of a core genome that contained 78% of all ORFs in P1/7.ConclusionsIn conclusion, we show that CGH is a valuable method to study distribution of genes or gene clusters among isolates in detail, yielding information on genetic similarity, and virulence traits of S. suis isolates.

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Section: Resultsmentioning

confidence: 99%

Genetic diversity of Streptococcus suis isolates as determined by comparative genome hybridization

et al. 2011

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“…The epf , mrp and sly genes have been shown to be significant virulence markers for S. suis that allow to differentiate virulent (presence) from less‐virulent (absence) strains in Europe and Asia (Schultsz et al., ). The presence of other additional genes, such as arc A, bay046 and hyl , has been also reported to be associated with S. suis virulence (Winterhoff et al., ; Okwumabua and Sharmila, ; Allen et al., ). The results of the present study revealed that the majority of serotype 2 isolates regardless of their origin (human or pig), epidemiological status (epidemic or sporadic) and disease category (diseased or asymptomatic pigs), carried all virulence‐associated genes tested (VAGP1).…”

Section: Discussionmentioning

confidence: 97%

“…Apart from the capsular polysaccharide (CPS), other putative virulence candidates, such as an extracellular protein factor (EF, encoded by the epf gene), a muramidase-released protein (MRP, encoded by the mrp gene), the suilysin (SLY, encoded by the sly gene), adhesins, an arginine deiminase (ArcA), a 38 kDa BAY046 protein (encoded by the bay046 gene) and an hyaluronidase (encoded by the hyl gene), have been suggested. However, their roles in the pathogenesis of human and swine infections remain poorly understood (Tikkanen et al, 1996;Gottschalk and Segura, 2000;Winterhoff et al, 2002;Allen et al, 2004;Okwumabua and Chinnapapakkagari, 2005;Fittipaldi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Virulence Genes and Genetic Diversity ofStreptococcus suisSerotype 2 Isolates from Thailand

Maneerat

Yongkiettrakul

Kramomtong

et al. 2013

Transbound Emerg Dis

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SummaryIsolates of Streptococcus suis from different Western countries as well as those from China and Vietnam have been previously well characterized. So far, the genetic characteristics and relationship between S. suis strains isolated from both humans and pigs in Thailand are unknown. In this study, a total of 245 S. suis isolates were collected from both human cases (epidemic and sporadic) and pigs (diseased and asymptomatic) in Thailand. Bacterial strains were identified by biochemical tests and PCR targeting both, the 16S rRNA and gdh genes. Thirty-six isolates were identified as serotype 2 based on serotyping and the cps2-PCR. These isolates were tested for the presence of six virulence-associated genes: an arginine deiminase (arcA), a 38-kDa protein and protective antigen (bay046), an extracellular factor (epf), an hyaluronidase (hyl), a muramidase-released protein (mrp) and a suilysin (sly). In addition, the genetic diversities of these isolates were studied by RAPD PCR and multilocus sequence typing (MLST) analysis. Four virulence-associated gene patterns (VAGP 1 to 4) were obtained, and the majority of isolates (32/36) carried all genes tested (VAGP1). Each of the three OPB primers used provided 4 patterns designated RAPD-A to RAPD-D. Furthermore, MLST analysis could also distinguish the 36 isolates into four sequence types (STs): ST1 (n = 32), ST104 (n = 2), ST233 (n = 1) and a newly identified ST, ST336 (n = 1). Dendrogram constructions based on RAPD patterns indicated that S. suis serotype 2 isolates from Thailand could be divided into four groups and that the characteristics of the individual groups were in complete agreement with the virulence gene profiles and STs. The majority (32/36) of isolates recovered from diseased pigs, slaughterhouse pigs or human patients could be classified into a single group (VAGP1, RAPD-A and ST1). This genetic information strongly suggests the transmission of S. suis isolates from pigs to humans in Thailand. Our findings are the first to report genetic characteristics of strains from Thailand and to elucidate the genetic relationship among S. suis isolates from human and pig origins.

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“…Hyaluronidase typically degrades HA and is relatively less active against CS. This type of hyaluronidase is generally the first subfamily of the PL-8 family, such as hyaluronidase from Streptococcus suis [29]. Chondroitin ABC lyase mainly degrades CS-A, CS-B, and CS-C.…”

Section: Discussionmentioning

confidence: 99%

Cloning, Expression, and Characterization of a New Glycosaminoglycan Lyase from Microbacterium sp. H14

et al. 2019

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Glycosaminoglycan (GAG) lyase is an effective tool for the structural and functional studies of glycosaminoglycans and preparation of functional oligosaccharides. A new GAG lyase from Microbacterium sp. H14 was cloned, expressed, purified, and characterized, with a molecular weight of approximately 85.9 kDa. The deduced lyase HCLaseM belonged to the polysaccharide lyase (PL) family 8. Based on the phylogenetic tree, HCLaseM could not be classified into the existing three subfamilies of this family. HCLaseM showed almost the same enzyme activity towards hyaluronan (HA), chondroitin sulfate A (CS-A), CS-B, CS-C, and CS-D, which was different from reported GAG lyases. HCLaseM exhibited the highest activities to both HA and CS-A at its optimal temperature (35 • C) and pH (pH 7.0). HCLaseM was stable in the range of pH 5.0-8.0 and temperature below 30 • C. The enzyme activity was independent of divalent metal ions and was not obviously affected by most metal ions. HCLaseM is an endo-type enzyme yielding unsaturated disaccharides as the end products. The facilitated diffusion effect of HCLaseM is dose-dependent in animal experiments. These properties make it a candidate for further basic research and application.

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Identification and characterisation of hyaluronate lyase from Streptococcus suis

Cited by 30 publications

References 22 publications

Genetic diversity of Streptococcus suis isolates as determined by comparative genome hybridization

Genetic diversity of Streptococcus suis isolates as determined by comparative genome hybridization

Virulence Genes and Genetic Diversity ofStreptococcus suisSerotype 2 Isolates from Thailand

Cloning, Expression, and Characterization of a New Glycosaminoglycan Lyase from Microbacterium sp. H14

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