Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment

Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp. Strain 176 Suppress the Motility of Vibrio alginolyticus

“…The mortality rates of the zebrafishes after injection were determined by directly counting the number of zebrafish that survived for 7 days postchallenge. The bacteria were reisolated and confirmed as the causative agents of mortality following Koch's postulates as described previously (46). All zebrafish experiments were carried out in accordance with the ethical guidelines of the Chinese Academy of Sciences.…”

Comparative Transcriptome Analysis of Vibrio splendidus JZ6 Reveals the Mechanism of Its Pathogenicity at Low Temperatures

“…Pathogenic V. splendidus strain JZ6 and nonpathogenic strain TZ19 for Yesso scallop (P. yessoensis) were employed in the present study (5). They were cultivated on Zobell 2216E agar at 18°C for 24 h, and the individual bacterial colonies were then subcultured in Trypticase soy broth (TSB) with 20‰ salinities at 10°C or 28°C and with shaking at 170 rpm for 12 h. After cultivation, bacterial cell numbers were adjusted to the same order of magnitude by absorption spectrophotometry and microscopic counting for the following experiments.…”

“…he Gram-negative pathogenic bacterium Vibrio splendidus is a ubiquitous and representative species of the Vibrio genus, a causal agent of vibriosis, which causes high rates of mortality in aquaculture animals, including turbot, scallop, clam, and oyster (1)(2)(3)(4)(5). Like most Vibrio strains, V. splendidus is an opportunistic pathogen that causes mortality of animals in an optimum environment, whereas it usually acts as a "normal bacterium" in the host or environment under adverse conditions (6)(7)(8).…”

“…Hence, temperature has been considered one of the crucial environmental factors that can regulate the metabolic process, growth, adhesion, and even pathogenicity of bacteria by influencing their gene expression (12)(13)(14). V. splendidus strain JZ6 was previously isolated and identified as a pathogenic agent for Yesso scallop (Patinopecten yessoensis) in low-temperature environments (5). This strain survived in cold environments and exhibited the highest pathogenicity at a temperature of 10°C.…”

CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules

“…In the present study, V. splendidus isolated from lesion-like niduses of moribund scallop Patinopecten yessoensis (Liu et al, 2013) was applied as stimuli agent, in which Vsm and OmpU were identified as main virulence factors. The bacteria was cultured in 2116E media at 18 C overnight, collected and washed in filter sterilized (0.22 mm pore size) seawater (FSSW), and finally re-suspended to the concentration of 2 Â 10 8 CFU ml À1 .…”