Identification and characterization of 14 polymorphic EST‐derived microsatellites in eelgrass (<i>Zostera marina</i>)

Oetjen, Katharina; Reusch, Thorsten B. H.

doi:10.1111/j.1471-8286.2007.01694.x

Cited by 26 publications

(11 citation statements)

References 7 publications

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“…We delineated genotypes and estimated relatedness using 11 microsatellite loci selected from a pool of >30 loci designed specifically for Zostera marina (Appendix ; see Reusch et al. , Reusch , Oetjen and Reusch , Oetjen et al. ).…”

Section: Methodsmentioning

confidence: 99%

The relative importance of trait vs. genetic differentiation for the outcome of interactions among plant genotypes

Abbott

Stachowicz

2016

Ecology

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Functional trait differences and genetic distance are increasingly used as metrics to predict the. outcome of species interactions and the maintenance of diversity. We apply these ideas to intraspecific diversity for the seagrass Zostera marina (eelgrass), by explicitly testing the influence of trait distance and genetic relatedness on the outcome of pairwise interactions among eelgrass genotypes. Increasing trait distance (but not relatedness) between eelgrass genotypes decreased the likelihood that both would persist over a year-long field experiment, contrary to our expectations based on niche partitioning. In plots in which one genotype excluded another, the biomass and growth of the remaining genotype increased with the trait distance and genetic relatedness of the initial pair, presumably due to a legacy of past interactions. Together these results suggest that sustained competition among functionally similar genotypes did not produce a clear winner, but rapid exclusion occurred among genotypes with distinct trait combinations. Borrowing from coexistence theory, we argue that fitness differences between genotypes with distinct traits overwhelmed any stabilizing effects of niche differentiation. Previously observed effects of eelgrass genetic diversity on performance may rely on nonadditive interactions among multiple genotypes or sufficient environmental heterogeneity to increase stabilizing forces and/or interactions.

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Section: Methodsmentioning

confidence: 99%

The relative importance of trait vs. genetic differentiation for the outcome of interactions among plant genotypes

Abbott

Stachowicz

2016

Ecology

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“…In total, 25 microsatellites of two different marker types were used in this study. We developed a first set of 14 gene‐linked microsatellites based on a Z. marina EST library containing sequences of 1103 unique genes (for details, see Oetjen & Reusch 2007), with putative gene functions based on a BLASTX search spanning from defence proteins to aquaporins (Table S2, Supplementary materials). They comprised eight di‐, five tri‐ and one tetra‐nucleotide repeat motifs.…”

Section: Methodsmentioning

confidence: 99%

“…These markers have proven to be highly polymorphic and easy to amplify in the study system and are thus suitable for population genetic approaches. Polymerase chain reaction (PCR) protocols followed Oetjen & Reusch (2007) and Reusch (2000). None of the newly developed nor already available markers were excluded from this study.…”

Section: Methodsmentioning

confidence: 99%

Genome scans detect consistent divergent selection among subtidal vs. intertidal populations of the marine angiosperm Zostera marina

Oetjen

Reusch

2007

Molecular Ecology

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Genome scans are a powerful tool to detect natural selection in natural populations among a larger sample of marker loci. We used replicated habitat comparisons to search for consistent signals of selection among contrasting populations of the seagrass Zostera marina, a marine flowering plant with important ecological functions. We compared two different habitat types in the North Frisian Wadden Sea, either permanently submerged (subtidal) or subjected to aerial exposure (intertidal). In three independent population pairs, each consisting of one tidal creek and one tidal flat population each, we carried out a genome scan with 14 expressed sequence tag (EST)-derived microsatellites situated in 5'- or 3'-untranslated regions of putative genes, in addition to 11 anonymous genomic microsatellites. By using two approaches for outlier identification, one anonymous and two EST-derived microsatellites showed population differentiation patterns not consistent with neutrality. These microsatellites were detected in several parallel population comparisons, suggesting that they are under diverging selection. One of these loci is linked to a putative nodulin gene, which is responsible for water channelling across cellular membranes, suggesting a functional link of the observed genetic divergence with habitat characteristics.

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“…We delineated genotypes and estimated relatedness using 11 microsatellite loci selected from a pool of >30 loci developed specifically for Z. marina (Abbott & Stachowicz, 2016; Oetjen, Ferber, Dankert & Reusch, 2010; Oetjen & Reusch, 2007; Reusch, 2000; Reusch, Stam & Olsen, 1999). We identified a total of 219 unique genotypes from the 260 ramets we collected.…”

Section: Methodsmentioning

confidence: 99%

Genetic distance predicts trait differentiation at the subpopulation but not the individual level in eelgrass,Zostera marina

Abbott

DuBois

Grosberg

et al. 2018

Ecology and Evolution

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Ecological studies often assume that genetically similar individuals will be more similar in phenotypic traits, such that genetic diversity can serve as a proxy for trait diversity. Here, we explicitly test the relationship between genetic relatedness and trait distance using 40 eelgrass (Zostera marina) genotypes from five sites within Bodega Harbor, CA. We measured traits related to nutrient uptake, morphology, biomass and growth, photosynthesis, and chemical deterrents for all genotypes. We used these trait measurements to calculate a multivariate pairwise trait distance for all possible genotype combinations. We then estimated pairwise relatedness from 11 microsatellite markers. We found significant trait variation among genotypes for nearly every measured trait; however, there was no evidence of a significant correlation between pairwise genetic relatedness and multivariate trait distance among individuals. However, at the subpopulation level (sites within a harbor), genetic (F ST) and trait differentiation were positively correlated. Our work suggests that pairwise relatedness estimated from neutral marker loci is a poor proxy for trait differentiation between individual genotypes. It remains to be seen whether genomewide measures of genetic differentiation or easily measured “master” traits (like body size) might provide good predictions of overall trait differentiation.

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Identification and characterization of 14 polymorphic EST‐derived microsatellites in eelgrass (Zostera marina)

Cited by 26 publications

References 7 publications

The relative importance of trait vs. genetic differentiation for the outcome of interactions among plant genotypes

The relative importance of trait vs. genetic differentiation for the outcome of interactions among plant genotypes

Genome scans detect consistent divergent selection among subtidal vs. intertidal populations of the marine angiosperm Zostera marina

Genetic distance predicts trait differentiation at the subpopulation but not the individual level in eelgrass,Zostera marina

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