Identification and Characterization of a Cis-Encoded Antisense RNA Associated with the Replication Process of Salmonella enterica Serovar Typhi

Dadzie, Isaac; Xu, Shengli; Ni, Bin; Zhang, Xiaolei; Zhang, Haifang; Sheng, Xiumei; Xu, Huaxi; Huang, Xinxiang

doi:10.1371/journal.pone.0061308

Cited by 13 publications

(11 citation statements)

References 38 publications

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“…coli , and asDnaA in Salmonella enterica . DicF inhibits translation of the cell-division protein FtsZ when overexpressed [ 62 ], while asdnaA is expressed in stationary phase and under other stress conditions and seems to increase stability of the dnaA mRNA by an unknown mechanism [ 63 ]. A few sRNAs have been reported to be involved in bacterial cell differentiation processes that may include modulation of cell cycle control.…”

Section: Discussionmentioning

confidence: 99%

A Stress-Induced Small RNA Modulates Alpha-Rhizobial Cell Cycle Progression

et al. 2015

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Mechanisms adjusting replication initiation and cell cycle progression in response to environmental conditions are crucial for microbial survival. Functional characterization of the trans-encoded small non-coding RNA (trans-sRNA) EcpR1 in the plant-symbiotic alpha-proteobacterium Sinorhizobium meliloti revealed a role of this class of riboregulators in modulation of cell cycle regulation. EcpR1 is broadly conserved in at least five families of the Rhizobiales and is predicted to form a stable structure with two defined stem-loop domains. In S. meliloti, this trans-sRNA is encoded downstream of the divK-pleD operon. ecpR1 belongs to the stringent response regulon, and its expression was induced by various stress factors and in stationary phase. Induced EcpR1 overproduction led to cell elongation and increased DNA content, while deletion of ecpR1 resulted in reduced competitiveness. Computationally predicted EcpR1 targets were enriched with cell cycle-related mRNAs. Post-transcriptional repression of the cell cycle key regulatory genes gcrA and dnaA mediated by mRNA base-pairing with the strongly conserved loop 1 of EcpR1 was experimentally confirmed by two-plasmid differential gene expression assays and compensatory changes in sRNA and mRNA. Evidence is presented for EcpR1 promoting RNase E-dependent degradation of the dnaA mRNA. We propose that EcpR1 contributes to modulation of cell cycle regulation under detrimental conditions.

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Section: Discussionmentioning

confidence: 99%

A Stress-Induced Small RNA Modulates Alpha-Rhizobial Cell Cycle Progression

et al. 2015

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“…For instance, one of the island-encoded sRNAs, IsrM, is important in the invasion by Salmonella of nonphagocytic cells [29]. Recently, we applied the RNA-seq technology to a transcriptomic analysis of S. Typhi, and found large numbers of putative ncRNAs, some of which were identified [15,30].…”

Section: Discussionmentioning

confidence: 99%

“…With an RNA-seq analysis of the transcriptome of S. Typhi under different environmental stresses, combined with bioinformatic tools, we have recently identified many putative novel ncRNAs in bacteria [15]. A 236-nt transcript partially encoded by the minus strand of bax was expressed and designated 'Asb' (antisense RNA of bax).…”

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confidence: 99%

5′-UTR of malS Increases the Invasive Capacity of Salmonella Enterica Serovar Typhi by Influencing the Expression of Bax

Gong

Jin

et al. 2015

Future Microbiol.

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“…The diversity of the cis-encoded ncRNA and their regulatory roles vary according to organisms as. For example, Salmonella enteric serovar Typhimurium possesses the cis-encoded ncRNA lesR-1 whose function is to control replication in eukaryotic cells [51] and Salmonella serovar Typi possesses the cis-encoded ncRNA AmgR involved in virulence in rats [52] and AsdA which regulates intracellular replication [53].…”

Section: Cis-encoded Ncrnasmentioning

confidence: 99%

Non-Coding RNAs in Bacteria

A¹,

Vl²,

T³

et al. 2018

Preprint

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Genes encoding regulatory RNAs known as short RNAs (sRNAs) or non-coding sRNAs (ncRNAs), modulate physiological responses through different mechanisms, through RNA-RNA interaction or RNA-protein interaction. These molecules transcribed in trans and in cis relative to the target RNA. They are located between the coding regions of proteins, i.e., in the intergenic regions of the genome and show signals of promoters and termini sequences generally Rho-independent. The size of the ncRNAs genes ranges from ~ 50 to ~ 500 nucleotides and several transcripts are processed by RNase with smaller end products, which modulate physiological responses through different mechanisms, by RNA-RNA interaction or RNA-protein interactions and some interactions may be stabilized by the Hfq chaperone. The Riboswitches constitute another class of ncRNAs, located in the 5'UTR region of an mRNA that promote transcriptional regulation through their interaction with a linker molecule. Recently, in prokaryotes, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) regions have described, which repeats of sequences of palindromic bases are. Each replicate consists of short segments of "spacer DNA" from exposures prior to a bacteriophage virus or exogenous plasmid. The CRISPR system consists of an immune system of resistance to exogenous molecules.

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Identification and Characterization of a Cis-Encoded Antisense RNA Associated with the Replication Process of Salmonella enterica Serovar Typhi

Cited by 13 publications

References 38 publications

A Stress-Induced Small RNA Modulates Alpha-Rhizobial Cell Cycle Progression

A Stress-Induced Small RNA Modulates Alpha-Rhizobial Cell Cycle Progression

5′-UTR of malS Increases the Invasive Capacity of Salmonella Enterica Serovar Typhi by Influencing the Expression of Bax

Non-Coding RNAs in Bacteria

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