Identification and characterization of a novel subtype of Tula virus in Microtus arvalis obscurus voles sampled from Xinjiang, China

Chen, Jintao; Qin, Jing; Li, Kun; Xu, Quangang; Wang, Xiaoping; Plyusnin, Alexander; Hou, Wei; Zhāng, Yǒng-Zhèn

doi:10.1016/j.meegid.2019.104012

Cited by 11 publications

(3 citation statements)

References 35 publications

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“…All captured rodents were first identified to species level by morphological examination. Rodent species were then confirmed by analyzing sequence of mitochondrial cytochrome b (mt-cyt b) gene (Guo et al, 2013;Chen et al, 2019). Tissue samples of heart, liver, spleen, lung, and kidney were collected from rodents to detect JMTV.…”

Section: Collection Of Animalsmentioning

confidence: 99%

Identification and characterization of Jingmen tick virus in rodents from Xinjiang, China

Chen

Qin

et al. 2020

Infection, Genetics and Evolution

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Jingmen tick virus (JMTV) is a recently identified virus which provides an unexpected connection between segmented and unsegmented RNA viruses. Recent investigations reveal that JMTV including JMTV-like virus (Alongshan virus) could be associated with human disease, suggesting the significance of JMTV in public health. To better understand the genetic diversity and host range of JMTV, a total of 164 rodents representing 8 species were collected in Qapqal Xibe county of Xinjiang Uygur Autonomous Region, China, and were screened for JMTVs using RT-PCR. Consequently, JMTV was identified in 42 rodents including 23 Microtus arvalis voles (24.5%), 9 Apodemus uralensis mice (29.0%), 5 Mus musculus mice, 1 Rhombomys opimus gerbil, 1 Meriones tamariscinus gerbil, 1 Meriones libycus gerbil, 1 Cricetulus migratorius hamster and 1 Microtus gregalis vole. Interestingly, nearly complete genome sequences were successfully recovered from 7 JMTV positive samples. Although the newly identified rodent JMTVs were closely related to those previously identified in ticks from China, based on the phylogenetic analysis of the S1, S2 and S3 segments, the newly identified rodent viruses clustered into two genetic groups. One group comprised of viruses only found in M. arvalis, while another group included viruses from A. uralensis, C. migratorius and M. gregalis. However, all rodent viruses clustered together in the S4 tree. Considering rodents live in close proximity to humans, more efforts are needed to investigate the role of rodents in the evolution and transmission of JMTV in nature.

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Section: Collection Of Animalsmentioning

confidence: 99%

Identification and characterization of Jingmen tick virus in rodents from Xinjiang, China

Chen

Qin

et al. 2020

Infection, Genetics and Evolution

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“…In general, published sequences for the L segment in this region are sparse and for the Almaty region, we describe for the first time also TULV L segment sequences, in comparison to a previous study that only analysed the S segment [22]. Sequences from Tekeli and Rudnichniy in Almaty region cluster in an individual branch in one big cluster with sequences from China and Turkey (Figure 3) [47,48]. This finding goes along with previous studies who have illustrated that genetic clustering of TULV is largely according to geographic regions [22,33].…”

Section: Discussionmentioning

confidence: 61%

Molecular Characterisation and Phylogeny of Tula Virus in Kazakhstan

Tukhanova

Shin

Turebekov

et al. 2022

Viruses

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Orthohantaviruses are zoonotic pathogens that play a significant role in public health. These viruses can cause haemorrhagic fever with renal syndrome in Eurasia. In the Republic of Kazakhstan, the first human cases were registered in the year 2000 in the West Kazakhstan region. Small mammals can be reservoirs of orthohantaviruses. Previous studies showed orthohantavirus antigens in wild-living small mammals in four districts of West Kazakhstan. Clinical studies suggested that there might be further regions with human orthohantavirus infections in Kazakhstan, but genetic data of orthohantaviruses in natural foci are limited. The aim of this study was to investigate small mammals for the presence of orthohantaviruses by molecular biological methods and to provide a phylogenetic characterization of the circulating strains in Kazakhstan. Small mammals were trapped at 19 sites in West Kazakhstan, four in Almaty region and at seven sites around Almaty city during all seasons of 2018 and 2019. Lung tissues of small mammals were homogenized and RNA was extracted. Orthohantavirus RT-PCR assays were applied for detection of partial S and L segment sequences. Results were compared to published fragments. In total, 621 small mammals from 11 species were analysed. Among the collected small mammals, 2.4% tested positive for orthohantavirus RNA, one sample from West Kazakhstan and 14 samples from Almaty region. None of the rodents caught in Almaty city were infected. Sequencing parts of the small (S) and large (L) segments specified Tula virus (TULV) in these two regions. Our data show that geographical distribution of TULV is more extended as previously thought. The detected sequences were found to be split in two distinct genetic clusters of TULV in West Kazakhstan and Almaty region. TULV was detected in the common vole (Microtus arvalis) and for the first time in two individuals of the forest dormouse (Dryomys nitedula), interpreted as a spill-over infection in Kazakhstan.

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“…2019 ; Hiltbrunner and Heckel 2020 ) as the reference. In addition, four published TULV genomes from Microtus obscurus from China ( Chen et al. 2019 ) and two published Puumala orthohantavirus genomes ( Vapalahti et al.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide support for incipient Tula hantavirus species within a single rodent host lineage

Labutin,

Heckel

2024

Virus Evolution

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Evolutionary divergence of viruses is most commonly driven by co-divergence with their hosts or through isolation of transmission after host-shifts. It remains mostly unknown, however, whether divergent phylogenetic clades within named virus species represent functionally equivalent byproducts of high evolutionary rates or rather incipient virus species. Here, we test these alternatives with genomic data from two widespread phylogenetic clades in Tula orthohantavirus (TULV) within a single evolutionary lineage of their natural rodent host, the common vole Microtus arvalis. We examined voles from 42 locations in the contact region between clades for TULV infection by RT-PCR. Sequencing yielded 23 TULV Central North and 21 TULV Central South genomes which differed by 14.9-18.5% at the nucleotide and 2.2-3.7% at the amino acid level without evidence of recombination or reassortment between clades. Geographic cline analyses demonstrated an abrupt (<1 km wide) transition between the parapatric TULV clades in continuous landscape. This transition was located within the Central mitochondrial lineage of M. arvalis and genomic SNPs showed gradual mixing of host populations across it. Genomic differentiation of hosts was much weaker across the TULV Central North to South transition than across the nearby hybrid zone between two evolutionary lineages in the host. We suggest that these parapatric TULV clades represent functionally distinct, incipient species which are likely differently affected by genetic polymorphisms in the host. This highlights the potential of natural viral contact zones as systems for investigating of the genetic and evolutionary factors enabling or restricting the transmission of RNA viruses.

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Identification and characterization of a novel subtype of Tula virus in Microtus arvalis obscurus voles sampled from Xinjiang, China

Abstract: Identification and characterization of a novel subtype of Tula virus in Microtus arvalis obscurus voles sampled from Xinjiang, China

Cited by 11 publications

References 35 publications

Identification and characterization of Jingmen tick virus in rodents from Xinjiang, China

Identification and characterization of Jingmen tick virus in rodents from Xinjiang, China

Molecular Characterisation and Phylogeny of Tula Virus in Kazakhstan

Genome-wide support for incipient Tula hantavirus species within a single rodent host lineage

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