Identification and characterization of a LuxI/R-type quorum sensing system in Pseudoalteromonas

Yu, Zhiliang; Yu, Dongliang; Mao, Yanfeng; Zhang, Mengting; Ding, Mengdan; Zhang, Jiadi; Wu, Shijin; Qiu, Juanping; Yin, Jianhua

doi:10.1016/j.resmic.2019.07.001

Cited by 20 publications

(15 citation statements)

References 65 publications

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“…The AHLs produced by E. coli and C. naphthovorans were extracted from culture supernatants as previously described [ 36 , 37 ]. 100 mL of overnight bacterial culture was centrifuged at 8000 rpm for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…The profile of AHLs was determined using thin layer chromatography (TLC) [ 36 ]. Briefly, the separation of AHL extracts was carried out on a TLC plate (TLC aluminum sheets, 20 cm × 20 cm, Silica gel 60 F254; Merck, Germany) using methanol–ultrapure water (60:40, v / v ) as mobile phase.…”

Section: Methodsmentioning

confidence: 99%

“…The AHLs produced by bacteria were measured by cross-feeding bioassay as described elsewhere [ 16 , 36 ]. The tested bacteria and the biosensor A. tumefaciens A136 were streaked in parallel on LB-P5Y3 plate (containing equal amounts of LB and P5Y3) supplemented with X-gal (40 μg/mL).…”

Section: Methodsmentioning

confidence: 99%

“…The broad-host-range plasmid pBBR1MCS-5 was used for the genetic complementation of mutants [ 36 ]. A fragment containing the gene of interest and its native promoter was amplified by PCR and then cloned into pBBR1MCS-5.…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA from C. naphthovorans strains was isolated using the RNAiso Plus Kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions. The qRT-PCR analysis was performed with a CFX Connect Real-Time PCR Detection System (BioRad, Hercules, CA, USA) as described previously [ 36 ]. The cycle threshold ( C T ) values for each gene of interest were normalized against the C T values of the 16S rRNA gene.…”

Section: Methodsmentioning

confidence: 99%

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The LuxI/LuxR-Type Quorum Sensing System Regulates Degradation of Polycyclic Aromatic Hydrocarbons via Two Mechanisms

et al. 2020

IJMS

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Members of the Sphingomonadales are renowned for their ability to degrade polycyclic aromatic hydrocarbons (PAHs). However, little is known about the regulatory mechanisms of the degradative pathway. Using cross-feeding bioassay, a functional LuxI/LuxR-type acyl-homoserine lactone (AHL)-mediated quorum sensing (QS) system was identified from Croceicoccus naphthovorans PQ-2, a member of the order Sphingomonadales. Inactivation of the QS system resulted in a significant decrease in PAHs degradation. The QS system positively controlled the expression of three PAH-degrading genes (ahdA1e, xylE and xylG) and a regulatory gene ardR, which are located on the large plasmid. Interestingly, the transcription levels of these three PAH-degrading genes were significantly down-regulated in the ardR mutant. In addition, bacterial cell surface hydrophobicity and cell morphology were altered in the QS-deficient mutant. Therefore, the QS system in strain PQ-2 positively regulates PAH degradation via two mechanisms: (i) by induction of PAH-degrading genes directly and/or indirectly; and (ii) by an increase of bacterial cell surface hydrophobicity. The findings of this study improve our understanding of how the QS system influences the degradation of PAHs, therefore facilitating the development of new strategies for the bioremediation of PAHs.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The LuxI/LuxR-Type Quorum Sensing System Regulates Degradation of Polycyclic Aromatic Hydrocarbons via Two Mechanisms

et al. 2020

IJMS

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Potential microbial functions and quorum sensing systems in partial nitritation and anammox processes

Feng

Sun

et al. 2021

Water Environment Research

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Diverse microbial communities coexist in the partial nitritation-anaerobic ammonium oxidation (PNA) process, in which nitrogen metabolism and information exchange are two important microbial interactions. In the PNA process, the existence of diverse microorganisms including nitrifiers, anammox bacteria, and heterotrophs makes it challenging to achieve a balanced relationship between anaerobic ammonium oxidation bacteria and ammonia oxidizing bacteria. In this study, potential microbial functions in nitrogen conversion and acyl-homoserine lactones (AHLs)-based quorum sensing (QS) in PNA processes were examined. Candidatus_Kuenenia and Nitrosomonas were the key functional bacteria responsible for PNA, while Nitrospira was detected as the dominant nitrite oxidizing bacteria (NOB). Heterotrophs containing nxr might play a similar function to NOB. The AHLs-QS system was an important microbial communication pathway in PNA systems. N-octanoyl-L-homoserine lactone, N-decanoyl homoserine lactone, and N-dodecanoyl homoserine lactone were the main AHLs, which might be synthesized by nitrogen converting microorganisms and heterotrophs. However, only heterotrophs had the potential to sense and degrade AHLs, such as Saccharophagus (sensing) and Leptospira (degradation). These results provide comprehensive information about the possible microbial functions and interactions in the PNA system and clues for system optimization from a microbial perspective. © 2021 Water Environment Federation • Practitioner points•Potential functions of anammox bacteria, nitrifiers, and heterotrophs were revealed.•Diverse nitrogen conversion and AHLs-quorum sensing related genes were detected.•Anammox bacteria and AOB played important roles in the AHLs synthesis process.•Heterotrophs could sense and degrade AHLs during information exchange.

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Molecular Relationships in Biofilm Formation and the Biosynthesis of Exoproducts in Pseudoalteromonas spp.

et al. 2022

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Identification and characterization of a LuxI/R-type quorum sensing system in Pseudoalteromonas

Cited by 20 publications

References 65 publications

The LuxI/LuxR-Type Quorum Sensing System Regulates Degradation of Polycyclic Aromatic Hydrocarbons via Two Mechanisms

The LuxI/LuxR-Type Quorum Sensing System Regulates Degradation of Polycyclic Aromatic Hydrocarbons via Two Mechanisms

Potential microbial functions and quorum sensing systems in partial nitritation and anammox processes

Molecular Relationships in Biofilm Formation and the Biosynthesis of Exoproducts in Pseudoalteromonas spp.

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