Identification and characterization of a cathepsin L-like cysteine protease from Taenia solium metacestode

Li, Ai Hua; Moon, Sung-Ung; Park, Yun-Kyu; Na, Byoung-Kuk; Hwang, Myung-Gi; Oh, Chang-Mi; Cho, Shin‐Hyeong; Kong, Yoon; Kim, Tong‐Soo; Chung, Pyung-Rim

doi:10.1016/j.vetpar.2006.05.015

Cited by 29 publications

(21 citation statements)

References 15 publications

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“…The clp gene of each Taenia parasite was cloned by PCR with forward primer 5Ј-ACATTTTCGTTTCGATCGGTCAT G-3Ј and reverse primer 5Ј-TGAACACATGGTTTAAACGTATGG-3Ј. Primers used were designed from conserved nucleotide sequences between Echinococcus multilocularis (21) and T. solium (10) clp genes to amplify almost the entire gene region. PCR was carried out using high-fidelity polymerase PrimeStar (Takara, Kyoto, Japan) in a final volume of 25 l reaction mixture containing 0.2 M of each primer, 200 M each of deoxynucleoside triphosphate (dNTP), 0.625 units of PrimeStar DNA polymerase, and genomic DNA, as described above.…”

Section: Methodsmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification Method for Differentiation and Rapid Detection of Taenia Species

Nkouawa

Sako²,

Nakao³

et al. 2009

J Clin Microbiol

112

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Rapid detection and differentiation of Taenia species are required for the control and prevention of taeniasis and cysticercosis in areas where these diseases are endemic. Because of the lower sensitivity and specificity of the conventional diagnosis based on microscopical examination, molecular tools are more reliable for differential diagnosis of these diseases. In this study, we developed and evaluated a loopmediated isothermal amplification (LAMP) assay for differential diagnosis of infections with Taenia species with cathepsin L-like cysteine peptidase (clp) and cytochrome c oxidase subunit 1 (cox1) genes. LAMP with primer sets to the cox1 gene could differentiate between three species, and LAMP with primer sets to the clp gene could differentiate Taenia solium from Taenia saginata/Taenia asiatica. Restriction enzyme digestion of the LAMP products from primer set Tsag-clp allowed the differentiation of Taenia saginata from Taenia asiatica. We demonstrated the high specificity of LAMP by testing known parasite DNA samples extracted from proglottids (n ‫؍‬ 100) and cysticerci (n ‫؍‬ 68). LAMP could detect one copy of the target gene or five eggs of T. asiatica and T. saginata per gram of feces, showing sensitivity similar to that of PCR methods. Furthermore, LAMP could detect parasite DNA in all taeniid egg-positive fecal samples (n ‫؍‬ 6). Due to the rapid, simple, specific, and sensitive detection of Taenia species, the LAMP assays are valuable tools which might be easily applicable for the control and prevention of taeniasis and cysticercosis in countries where these diseases are endemic.

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Section: Methodsmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification Method for Differentiation and Rapid Detection of Taenia Species

Nkouawa

Sako²,

Nakao³

et al. 2009

J Clin Microbiol

112

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“…This protease, produced in recombinant form, had antigenic activity recognized by sera from patients with NCC. 76 Two other protease fractions highly abundant in cystic fluid have been isolated and evaluated in ELISA and EITB with promising results, and in dot blot form, with lower sensitivity, albeit higher specificity. 77,78 Synthetic peptide production is appealing for its ease of production and inherent reproducibility.…”

Section: -24mentioning

confidence: 99%

Immunological and molecular diagnosis of cysticercosis

Rodríguez¹,

Wilkins

Dorny

2012

Pathogens and Global Health

137

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Cysticercosis, the infection with the larval stage of Taenia solium, is a cause of neurological symptoms including seizures, affecting the quality of life of patients and their families. Diagnosis focuses on brain imaging and serological tests are mostly used as confirmatory tools. Most cases, however, occur in poor endemic areas, where both kinds of diagnostic tools are poorly available. Development of point of care diagnostic tests is one of the most important priorities for cysticercosis researches today. The ideal point of care test would require detection of viable cysticercosis and hopefully identify cases with severe or progressive forms of neurocysticercosis, leading to referral of the patient for specialized medical attention. This manuscript describes the evolution of the serological diagnosis of cysticercosis over time, and the characteristics of the most common currently available tools, their advantages and disadvantages, and their potential use in future diagnostic tests.

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“…Besides, it is becoming obvious that parasite inhibitors may control the host's immune response (Dzik, 2006;Knox, 2007). Proteinase inhibitors, as well as proteinases themselves, play an important role in the life cycle of parasites, their virulence and pathogenesis (Li et al, 2006;Hwang et al, 2009;Molehin et al, 2012;Rascón & McKerrow, 2013). Inhibitors are produced by parasites to avoid proteolysis by host proteinases and thus survive.…”

Section: Introductionmentioning

confidence: 99%

“…In some cases the obtained data are contradictory. While inhibitors of all classes of proteinases (aspartyl, serine, cysteine and metalloproteinases) occurring at various stages of parasite life cycle have been discovered in nematodes (Hawley, Peanasky, 1992;Kageyama, 1998;Delaney et al, 2005;Knox, 2007), only inhibitors of serine proteinases (trypsin and chymotrypsin) have been found in cestodes (Matskási, 1984;Pappas, Uglem, 1990;Li et al 2006) so far. It may be due to the differences in the life cycle of these groups of helminths and, consequently, the barriers bro-ken by the parasites to get into the host, as well as to insuffi cient information on inhibitors in cestodes.…”

Section: Introductionmentioning

confidence: 99%

Adsorption and inactivation of proteolytic enzymes by Triaenophorus nodulosus (Cestoda)

2017

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SummaryThe proteolytic activity in washings off the Triaenophorus nodulosus cestode tegument and the ability of the worms to inactivate proteolytic enzymes were studied. It was found that the major proteolytic activity in the washing samples is represented by the easily desorbed fraction most probably characterizing the activity of the host's enzymes. Serine proteinases are an essential part of these enzymes. It was shown that the worms' incubation medium and their homogenates can inhibit host proteinases and commercial trypsin samples. Suppressive activity of the incubation medium suggests that the inhibitors are rather spontaneously produced by the worms than induced by the presence of proteinases in the surrounding medium. The inhibitor produced by the cestode is hypothesized to be trypsin-specifi c.

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Identification and characterization of a cathepsin L-like cysteine protease from Taenia solium metacestode

Cited by 29 publications

References 15 publications

Loop-Mediated Isothermal Amplification Method for Differentiation and Rapid Detection of Taenia Species

Loop-Mediated Isothermal Amplification Method for Differentiation and Rapid Detection of Taenia Species

Immunological and molecular diagnosis of cysticercosis

Adsorption and inactivation of proteolytic enzymes by Triaenophorus nodulosus (Cestoda)

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