Identification and characterization of a novel natural recombinant avian leucosis virus from Chinese indigenous chicken flock

Liang, Xiongyan; Gu, Yajia; Chen, Xueyang; Li, Tuofan; Gao, Yulong; Wang, Xiaomei; Fang, Chun; Fang, Shouguo; Yang, Yuying

doi:10.1007/s11262-019-01695-7

Cited by 25 publications

(24 citation statements)

References 33 publications

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“…In this study, we show the relatively low replication efficiency of ALV-K due to its weak LTR promoter activity, which may produce false negatives in routine surveillance by ELISA or virus isolation assay. More seriously, ALV-K strains with an endogenous-like U3 region and a replication rate even lower than that of JS11C1 have been isolated in Chinese indigenous breeds in recent years [44]. Despite the low pathogenic characteristics of ALV-K, it remains capable of inducing growth retardation and immunosuppression, as shown in this study.…”

Section: Discussionmentioning

confidence: 60%

Role of env gene and LTR sequence in the pathogenesis of subgroup K avian leukosis virus

Liu

Lin

et al. 2022

Journal of General Virology

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Avian leukosis virus (ALV) is a retrovirus that induces tumours in infected birds; ALV is divided into different subgroups according to the env gene and cellular tropism. In general, ALV subgroup J (ALV-J) is considered to be the most pathogenic and prevalent subgroup while subgroup K (ALV-K), a newly identified subgroup, only causes mild symptoms. To illuminate the roles of the env viral gene and LTR sequence in pathogenic differences between ALV-J and ALV-K, rescued ALV-J strain rSDAU1005, rescued ALV-K strain rJS11C1, and recombinant strains rENV(J)-LTR(K) and rENV(K)-LTR(J) were characterized and investigated in this study. Among rescued viruses, rSDAU1005 had the highest replication efficiency while rJS11C1 replicated the slowest (replication efficiency rankings were rSDAU1005 >rENV(K)-LTR(J)>rENV(J)-LTR(K)>rJS11 C1). The luciferase reporter gene assay results showed that the promoter activity of ALV-K LTR was lower than that of the ALV-J LTR promoter, which may have accounted for the slower replication efficiency of ALV-K. Pathogenicity of the four rescued viruses was determined via inoculating the yolk sacs of specific-pathogen-free chickens. The results demonstrated that all four viruses were pathogenic; rSDAU1005 caused the most severe growth retardation and immunosuppression. rENV(J)-LTR(K) was more pathogenic when compared to rENV(K)-LTR(J), indicating that env and the LTR sequence play important roles in pathogenicity between ALV-K and ALV-J. Additionally, env seemed to especially play a role in ALV-K pathogenesis. This study provided scientific data and insight to improve detection methods and judgement criteria in ALV clearance and surveillance.

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Section: Discussionmentioning

confidence: 60%

Role of env gene and LTR sequence in the pathogenesis of subgroup K avian leukosis virus

Liu

Lin

et al. 2022

Journal of General Virology

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“…Recently, novel ALV-J strains with large mutations or deletion in genome have been isolated [ 7 ]. In addition to mutation or deletion, recombination among different ALV subgroups or strains has also been frequently reported [ 8 , 9 ]. A novel ALV strain JS15SG01 with the recombination of the ALV-K, ALV-E and ALV-J, which could even invade and injury brain tissue, has been recently identified [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Chicken hepatomegaly and splenomegaly associated with novel subgroup J avian leukosis virus infection

Hang

Qian

et al. 2022

BMC Vet Res

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Background Subgroup J avian leukosis virus (ALV-J) is an oncovirus which can induce multiple types of tumors in chicken. In this report, we found novel ALV-J infection is closely associated with serious hepatomegaly and splenomegaly in chicken. Case presentation The layer chickens from six flocks in Jiangsu province, China, showed serious hemoperitoneum, hepatomegaly and splenomegaly. Histopathological results indicated focal lymphocytic infiltration, cell edema and congestion in the liver, atrophy and depletion of lymphocyte in the spleen. Tumor cells were not detected in all the organs. avian hepatitis E virus (aHEV), which is thought to be the cause of a very similar disease, big liver and spleen disease (BLS), was not detected. Other viruses causing tumors or liver damage including Marek’s disease virus (MDV), reticuloendotheliosis virus (REV), fowl adenovirus (FAdV) and chicken infectious anemia virus (CIAV) were also proved negative by either PCR or RT-PCR. However, we did detect ALV-J in those chickens using PCR. Only novel ALV-J strains were efficiently isolated from these chicken livers. Conclusions This is the first report that chicken hepatomegaly and splenomegaly disease was closely associated with novel ALV-J, highlighting the importance of ALV-J eradication program in China.

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“…According to the characteristics of host range, viral envelope protein, and cross-neutralization patterns, ALVs can be classified as endogenous or exogenous viruses. Exogenous ALVs can be classified into subgroups (A, B, C, D, J and K) in the chicken, which can cause different pathological lesions in chickens( Liang et al., 2019 ; Chang et al., 2020 ). Compared with other subgroups of ALVs, ALV-J mainly causes hematopoietic malignancy with myeloid leukemia and hemangioma in the chicken ( Cheng et al., 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Preparation of a novel monoclonal antibody against Avian leukosis virus subgroup J Gp85 protein and identification of its epitope

Wang

Chen²,

Zhu³

et al. 2021

Poultry Science

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Avian leukosis virus subgroup J ( ALV-J ) is an avian oncogenic retrovirus that has caused huge economic losses in the poultry industry due to its great pathogenicity and transmission ability. However, the continuous emergence of new strains would bring challenges to diagnosis and control of ALV-J. .This study focuses on preparing the monoclonal antibody ( MAb ) against ALV-J Gp85 and identifying its epitope. The truncated ALV-J gp85 gene fragment was amplified and then cloned into expression vectors. Purified GST-Gp85 was used to immune mice and His-Gp85 was used to screen MAb. Finally, a hybridoma cell line named J16 that produced specific MAb against the ALV-J. Immunofluorescence assay showed that MAb J16 specifically recognized ALV-J rather than ALV-A or ALV-K infected DF-1 cells. To identify the epitope recognized by MAb J16, fourteen partially overlapping ALV-J Gp85 fragments were prepared and tested by Western blot. The results indicated that peptide 150-LIRPYVNQ-157 was the minimal epitope of ALV-J Gp85 recognized by MAb J16. Alignment analysis of Gp85 from different ALV subgroups showed that the epitope keep high conservation among 36 ALV-J strains, but significant different from that of ALV subgroup A, B, C, D, E and K. Overall, we prepared a MAb specific against ALV-J and identified peptide 150-LIRPYVNQ-157 as a novel specific epitope of ALV-J Gp85, which may assist in laying the foundation for specific ALV-J detection methods.

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Identification and characterization of a novel natural recombinant avian leucosis virus from Chinese indigenous chicken flock

Cited by 25 publications

References 33 publications

Role of env gene and LTR sequence in the pathogenesis of subgroup K avian leukosis virus

Role of env gene and LTR sequence in the pathogenesis of subgroup K avian leukosis virus

Chicken hepatomegaly and splenomegaly associated with novel subgroup J avian leukosis virus infection

Preparation of a novel monoclonal antibody against Avian leukosis virus subgroup J Gp85 protein and identification of its epitope

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