Identification and characterization of a RAPD‐PCR marker for distinguishing Asian and North American gypsy moths

Garner, Karen J.; Slavicek, James M.

doi:10.1111/j.1365-2583.1996.tb00043.x

Cited by 53 publications

(29 citation statements)

References 15 publications

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“…SCAR markers have been successfully developed for the detection of gut contents from the tobacco budworm, Helicoverpa armigera (Agusti et al, 1999) and the whitefly, Trialeurodes vaporariorum (Agusti et al, 2000). SCAR markers have also been used to differentiate between different strains of the Asian rice gall midge, Orseolia oryzae (Behura et al, 1999), and to distinguish the Asian from the North American gypsy moth, Lymantria dispar (Garner and Slavicek, 1996). Similarly, SCARs have been developed for tagging genes in plants that confer resistance against insects, fungi, and nematodes (Paran and Michelmore 1993;Williamson et al, 1994;Nair et al, 1995Nair et al, , 1996.…”

Section: April 2003mentioning

confidence: 98%

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Development of scar markers for the dna‐based detection of the Asian long‐horned beetle, Anoplophora glabripennis (Motschulsky)

Kethidi

Roden

Ladd

et al. 2003

Arch Insect Biochem Physiol

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DNA markers were identified for the molecular detection of the Asian long-horned beetle (ALB), Anoplophora glabripennis (Mot.), based on sequence characterized amplified regions (SCARs) derived from random amplified polymorphic DNA (RAPD) fragments. A 2,740-bp DNA fragment that was present only in ALB and not in other Cerambycids was identified after screening 230 random primers in a PCR-based assay system. Three pairs of nested 22-mer oligonucleotide primers were designed on the basis of the sequence of this fragment and were used to perform diagnostic PCR. The first pair of primers (SCAR1) amplified a single 745-bp fragment of ALB DNA, but this did not differentiate ALB from other species. The other two pairs of SCAR primers (SCAR2 and SCAR3) amplified bands of 1,237- and 2,720-bp, respectively, that were capable of differentiating ALB from other closely related non-native and native Cerambycids, such as A. chinensis (Forster), A. malasiaca (Thomson), A. nobilis (Ganglbauer), Monochamus scutellatus (Say), Plectrodera scalator (Fab), Saperda tridentata (Olivier), and Graphisurus fasciatus (Degeer). The latter two SCAR markers could be amplified using DNA extracted from body parts of ALB such as the wing, the leg, and the antennae as well as tissues from all the developmental stages including the egg, larva, pupa, and adult. These markers were also capable of identifying ALB using the DNA extracted from frass. Our results demonstrate that the SCAR markers we have identified can be used for unambiguously identifying ALB from other closely related Cerambycids using a simple PCR procedure.

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Section: April 2003mentioning

confidence: 98%

“…SCARs have been used as genetic markers to distinguish Asian gypsy moths from the established North American populations (Garner and Slavicek, 1996). SCAR markers have also been used for identifying different species and biotypes of Asian gall midge (Behura et al, 1999), a major insect pest of rice.…”

Section: Introductionmentioning

confidence: 99%

Development of scar markers for the dna‐based detection of the Asian long‐horned beetle, Anoplophora glabripennis (Motschulsky)

Kethidi

Roden

Ladd

et al. 2003

Arch Insect Biochem Physiol

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“…In contrast to the flightless female EGM, AGM females can disperse up to 30 km (Wallner 1996). EGM and AGM are distinguishable by genetic markers although hybrids of the two can also form (Garner and Slavicek 1996). The gypsy moth is not host specific, and this has contributed to its extensive spread.…”

Section: Introductionmentioning

confidence: 95%

Risk assessment of the gypsy moth, Lymantria dispar (L), in New Zealand based on phenology modelling

2006

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The gypsy moth is a global pest that has not yet established in New Zealand despite individual moths having been discovered near ports. A climate-driven phenology model previously used in North America was applied to New Zealand. Weather and elevation data were used as inputs to predict where sustainable populations could potentially exist and predict the timing of hatch and oviposition in different regions. Results for New Zealand were compared with those in the Canadian Maritimes (New Brunswick, Nova Scotia, and Prince Edward Island) where the gypsy moth has long been established. Model results agree with the current distribution of the gypsy moth in the Canadian Maritimes and predict that the majority of New Zealand's North Island and the northern coastal regions of the South Island have a suitable climate to allow stable seasonality of the gypsy moth. New Zealand's climate appears more forgiving than that of the Canadian Maritimes, as the model predicts a wider range of oviposition dates leading to stable seasonality. Furthermore, we investigated the effect of climate change on the predicted potential distribution for New Zealand. Climate change scenarios show an increase in probability of establishment throughout New Zealand, most noticeably in the South Island.

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“…Pfeifer et al [5]employed a specific primer ITS22 of nDNA that revealed fragment length polymorphisms among geographical populations of gypsy moths. A RAPD-PCR marker (FS21) was designed to distinguish the North American and Asian gypsy moth populations [11]. Similarly Schreiber et al [6] used 3 RAPD-PCR markers (FS22, FS23 and FS24) for the same purpose.…”

Section: Coi Gene Amplification Sequence Characteristics and Geograpmentioning

confidence: 99%

“…Variations of microsatellite DNA and mitochondrial DNA (mtDNA) were used to track gypsy moth sources [3][4][5]. Asian and North American populations were differentiated by an amplified ribosomal DNA restriction analysis [11]. More recent, RAPD (random amplified polymorphic DNA) and AFLP (amplified fragment length polymorphism) technology were used as molecular markers for gypsy moth population diagnostics [7].…”

Section: Sequence and Computational Analysismentioning

confidence: 99%

COI gene geographic variation of Gypsy moth (Lepidoptera: Lymantriidae) and a TaqMan PCR diagnostic assay

Lu¹,

An²,

Song³

et al. 2014

DNA Barcodes

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Gypsy moth, an important forest/urban pest worldwide, is separated into the European and Asian subspecies, and has important quarantine significance. Diagnostic technique that can accurately and quickly distinguish subspecies is lacking. This study aimed to evaluate genetic difference between the subspecies, and subsequently to develop a reliable and high throughput molecular based diagnostic tool for distinguishing the subspecies. COI genes of 25 gypsy moth samples from China, Russia, Mongolia, Japan and the United States were sequenced. DNASTAR analysis revealed that gypsy moth COI gene was 1531bp long. The UPGM phylogenetic tree constructed based on the COI gene indicated that European subspecies (U.S. population) and Asian subspecies were distinctively divided into two branches. Japanese populations had a far distantly relationship with other Asian populations forming a separate branch. There was a single base substitution (base transformation only) at 14 consistent locations between Asian and American populations, but 13 of them coded the same amino acid. A MGB proper and TaqMan assay was designed base on the base substitution at 406th bp that coded a different amino acid. This allele typing assay took only 4 hours and could accurately distinguish gypsy moth subspecies of Europe and Asia. The study enriches the knowledge basis of genetic differentiation of gypsy moth subspecies. And more importantly the TaqMan assay is the first report of such diagnostic tool that could deliver rapid and accurate results and suitable for routine quarantine inspections to distinguish Asian and European gypsy moth subspecies. This study was supported by the Ministry of Science and Technology of the People’s Republic of China (Science and technology supporting project: 2012BAK11B03; International cooperation project: 2009DFA31950) and Jiangsu Entry and Exit Inspection and Quarantine Bureau (2014KJ45).

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Identification and characterization of a RAPD‐PCR marker for distinguishing Asian and North American gypsy moths

Cited by 53 publications

References 15 publications

Development of scar markers for the dna‐based detection of the Asian long‐horned beetle, Anoplophora glabripennis (Motschulsky)

Development of scar markers for the dna‐based detection of the Asian long‐horned beetle, Anoplophora glabripennis (Motschulsky)

Risk assessment of the gypsy moth, Lymantria dispar (L), in New Zealand based on phenology modelling

COI gene geographic variation of Gypsy moth (Lepidoptera: Lymantriidae) and a TaqMan PCR diagnostic assay

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