Identification and Characterization of a Newly Isolated Chitinase-Producing Strain Bacillus licheniformis SSCL-10 for Chitin Degradation

Sasi, Abirami; Duraipandiyan, Nagarajan; Kannan, Marikani; Dhanasekaran, Sugapriya; Al-Dayan, Noura; Venugopal, Divya

doi:10.1155/2020/8844811

Cited by 14 publications

(6 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, random mutation can also effectively improve the activity of chitinase. The activity of chitinase from Bacillus licheniformis was increased by five to six times through chemical and ultraviolet (UV) mutagenesis [75]. Gomaa [76] obtained six mutant strains through γ-irradiation on Bacillus thuringiensis NM101-19, of which the BM15 mutant showed the highest enzymatic activity of 65.41 U/mL.…”

Section: Enzymatic Methodsmentioning

confidence: 99%

Chitin oligosaccharides for the food industry: production and applications

Zhang

Zhao

et al. 2022

Syst Microbiol and Biomanuf

View full text Add to dashboard Cite

Chitin oligosaccharides (CHOS), high-value-added oligomers linked by N-acetyl-d-glucosamine (GlcNAc, NAG), and a small amount of d-glucosamine (GlcN, GA), have aroused increasing interest due to their excellent biological properties, including antibacterial, anti-inflammatory, and immunoprotective activities, and intestinal regulation. The efficient production and utilization of CHOS with high performance can solve problems from chitin as biowaste. However, the large-scale production of well-defined CHOS has not been fully accomplished due to the limited biotechnology and separation methods, thus impeding the research on their biological functions as well as their accurate applications. In this review, we comprehensively summarize the current preparation methods of CHOS, including the chemical, physical, enzymatic and biosynthetic methods. The advantages and disadvantages of the methods are discussed in terms of efficiency, economy, and environmental effects. Furthermore, the applications of CHOS in the food industry and their contributions to human health based on their excellent bioactivities are expounded. It is hoped that this review will help in providing new insights into the production of CHOS with high precision, and support the application of CHOS in serving the food industry as nutritional supplements or foods for special medical purposes. KeywordsChitin • Chitin oligosaccharides • Chitooligosaccharides • Production • Food applications Abbreviations CHOS Chitin oligosaccharides; N-acetyl chitooligosaccharides COS Chitooligosaccharides GlcNAc, NAG N-Acetyl-d-glucosamine GlcN, GA d-Glucosamine

show abstract

Section: Enzymatic Methodsmentioning

confidence: 99%

Chitin oligosaccharides for the food industry: production and applications

Zhang

Zhao

et al. 2022

Syst Microbiol and Biomanuf

View full text Add to dashboard Cite

show abstract

“… A. hydrophila NRRL B445 2.1.3. Class III: heat-stable and heat-labile proteins larger than 10 kDa BLIS_SXAU06 B. licheniformis SXAU06 14 kDa S. aureus S. epidermidis M. luteus L. monocytogenes [ 66 ] BL-DZ1 (BL00275) B. licheniformis strain D1 14 kDa Bacillus pumilus TiO1 Candida albicans BH [ 67 ] Pseudomonas aeruginosa PAO1 (biofouling) YbdN Protein B. licheniformis (seaweed isolate) 30.7 kDa MRSA 9551 MRSA J2407 VRE 788 VRE 1349 L. monocytogenes NCTC 7973, NCTC 10357 [ 68 ] Chitinase B. licheniformis MY75 (also produced by Mb-2, TP–1, S213, SSCL-10, B307 strains) 55 (67,68/62/60,65,66) kDa G. saubinetii A. niger ( Phoma medicaginis ) [ 69 , 70 , 71 , 72 , 73 , 74 , 75 ] Antifungal Protein F2 B. licheniformis BS-3 31 kDa Aspergillus niger Magnaporthe oryzae Rhizoctonia solani ...…”

Section: Antibacterial Substances Secreted By Bacillus Lich...mentioning

confidence: 99%

“…The secretion of this protein was induced by chitin powder [ 69 ]. Chitinase proteins were present in the culture supernatant of B. licheniformis Mb-2 [ 74 ], B. licheniformis TP–1 [ 75 ], B. licheniformis S213 [ 70 ], B. licheniformis SSCL-10 [ 71 ], B. licheniformis B307 [ 72 ].…”

Section: Antibacterial Substances Secreted By Bacillus Lich...mentioning

confidence: 99%

Bacillus licheniformis: A Producer of Antimicrobial Substances, including Antimycobacterials, Which Are Feasible for Medical Applications

Shleeva,

Kondratieva,

Kaprelyants

2023

Pharmaceutics

View full text Add to dashboard Cite

Bacillus licheniformis produces several classes of antimicrobial substances, including bacteriocins, which are peptides or proteins with different structural composition and molecular mass: ribosomally synthesized by bacteria (1.4–20 kDa), non-ribosomally synthesized peptides and cyclic lipopeptides (0.8–42 kDa) and exopolysaccharides (>1000 kDa). Different bacteriocins act against Gram-positive or Gram-negative bacteria, fungal pathogens and amoeba cells. The main mechanisms of bacteriocin lytic activity include interaction of peptides with membranes of target cells resulting in structural alterations, pore-forming, and inhibition of cell wall biosynthesis. DNase and RNase activity for some bacteriocines are also postulated. Non-ribosomal peptides are synthesized by special non-ribosomal multimodular peptide synthetases and contain unnatural amino acids or fatty acids. Their harmful effect is due to their ability to form pores in biological membranes, destabilize lipid packaging, and disrupt the peptidoglycan layer. Lipopeptides, as biosurfactants, are able to destroy bacterial biofilms. Secreted polysaccharides are high molecular weight compounds, composed of repeated units of sugar moieties attached to a carrier lipid. Their antagonistic action was revealed in relation to bacteria, viruses, and fungi. Exopolysaccharides also inhibit the formation of biofilms by pathogenic bacteria and prevent their colonization on various surfaces. However, mechanism of the harmful effect for many secreted antibacterial substances remains unknown. The antimicrobial activity for most substances has been studied in vitro only, but some substances have been characterized in vivo and they have found practical applications in medicine and veterinary. The cyclic lipopeptides that have surfactant properties are used in some industries. In this review, special attention is paid to the antimycobacterials produced by B. licheniformis as a possible approach to combat multidrug-resistant and latent tuberculosis. In particular, licheniformins and bacitracins have shown strong antimycobacterial activity. However, the medical application of some antibacterials with promising in vitro antimycobacterial activity has been limited by their toxicity to animals and humans. As such, similar to the enhancement in the antimycobacterial activity of natural bacteriocins achieved using genetic engineering, the reduction in toxicity using the same approach appears feasible. The unique capability of B. licheniformis to synthesize and produce a range of different antibacterial compounds means that this organism can act as a natural universal vehicle for antibiotic substances in the form of probiotic cultures and strains to combat various types of pathogens, including mycobacteria.

show abstract

“…Bacteria produce chitinases to obtain nitrogen and carbon as a source of nutrients or precursors and parasitism (Rathore and Gupta 2015). Several genera of bacteria, including Serratia (Bahar et al 2012), Bacillus (Sasi et al 2020), Streptomyces (Tran et al 2019), Enterobacter (Sivaramakrishna et al 2020), and Vibrio (Jahromi and Barzkar 2018), produce chitinase. A few viral chitinases, mainly species from Baculoviridae, are currently known (Ishimwe et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Comparison of the potential activities of viral and bacterial chitinases

Abulikemu

Yesilyurt

Gencer

et al. 2021

Egypt J Biol Pest Control

View full text Add to dashboard Cite

Background Chitin, a long-chain polymer of N-acetylglucosamine, is a major structural component of the insect exoskeleton and the peritrophic membrane (PM). Chitinases are able to effectively break down glycosidic bonds of chitin polymer thus can be used in agriculture to control plant pathogen insects. These enzymes can be synthesized by higher plants, animals, protista, bacteria, and viruses. Results In this study, viral and bacterial chitinases were compared for their potential activity on a laboratory test insect. The genes encoding chitinases of Autographa californica nucleopolyhedrovirus (AcNPV) and Cydia pomonella granulovirus (CpGV) were amplified from genomic DNAs by PCR and cloned into the pET-28a (+) expression vector. The chitinase proteins of these 2 viruses (AcNPV-Chi, CpGV-Chi) and Serratia marcescens chitinase C (ChiC) protein which was previously cloned were overexpressed in Escherichia coli. Expressed proteins were purified and confirmed by western blot analysis as 50, 63, and 68 kDa for AcNPV, CpGV, and S. marcescens chitinases, respectively. Enzyme activities of the chitinases were confirmed. Chitinases were also compared to each other in silico. The insecticidal effects of these proteins were evaluated on Galleria mellonella L. larvae. Bioassays were performed on the 3rd instar larvae for each chitinase protein in triplicate. The results showed that although there were differences in enzymatic activities and domain organizations, all 3 microbial chitinases produced almost the same level of insecticidal activity on the test insect. LC50 and LT50 values were compatible with the mortality results. These results were a preanalysis for comparing the effects of microbial chitinases. Conclusion Potential activity experiments should be carried out on more insects to provide detailed information on the insecticidal effects of bacterial and viral chitinases.

show abstract

Identification and Characterization of a Newly Isolated Chitinase-Producing Strain Bacillus licheniformis SSCL-10 for Chitin Degradation

Cited by 14 publications

References 38 publications

Chitin oligosaccharides for the food industry: production and applications

Chitin oligosaccharides for the food industry: production and applications

Bacillus licheniformis: A Producer of Antimicrobial Substances, including Antimycobacterials, Which Are Feasible for Medical Applications

Comparison of the potential activities of viral and bacterial chitinases

Contact Info

Product

Resources

About