Identification and characterization of a clam ferritin from Sinonovacula constricta

Li, Chenghua; Li, Hui; Su, Xiurong; Li, Taiwu

doi:10.1016/j.fsi.2011.02.017

Cited by 33 publications

(15 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The molecular mass of the purified product was in good agreement with the predicted molecular weight of ScFER, AjFER, and AlFER. These three protein bands had molecular weights between 20.1 and 29 kDa and were detected in the positive transformants in our previous work[36–38]. Here, recombinant ferritin from S .…”

Section: Resultssupporting

confidence: 55%

“…japonicus , and A . Leucoprocta were expressed and purified as experimental materials, and the correctness was verified by western blot in the past[36–38]. The intensity of the recombinant protein bands increased with time, and peak expression of recombinant ferritin occurred 5 h after IPTG was introduced.…”

Section: Resultsmentioning

confidence: 99%

“…japonicus , and A . leucoprocta [36–38]. These three benthic animals are widely distribution in coastal areas of China.…”

Section: Discussionmentioning

confidence: 99%

“…The ferritins which were critical to participate in the physiological function had high homology in the bodies[1, 2]. Their interiors had no iron core frameworks supporting[36, 37]. Recombinant ferritins which were more flexible with higher plasticity mean new empty cavities, could maximize the adsorption of manganese[40].…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Preparation and Representation of Recombinant Mn-Ferritin Flower-Like Spherical Aggregates from Marine Invertebrates

et al. 2015

Self Cite

View full text Add to dashboard Cite

Ferritin has important functions in the transition and storage of toxic metal ions, but its regulation and function in many invertebrate species are still largely unknown. In our previous work, the cDNA sequence of Sinonovacula constricta, Apostichopus japonicas and Acaudina leucoprocta were constructed and efficiently expressed in E. Coli BL21 under IPTG induction. In this follow-up study, the recombinant ferritins were exposed to heavy metal manganese. The manganese concentration levels in three recombinant ferritins were greater than horse spleen ferritin (HSF). Compared with HSF, the amount of manganese enrichment in the three recombinant ferritins was 1.75-fold, 3.25-fold and 2.42-fold increases in ScFER, AjFER, and AlFER, respectively. After phosphate stimulation, the concentration of manganese increased and was higher than the ordinary dialysis control groups. The ScFER was four times its baseline value. The AjFER and AlFER were 1.4- and 8-fold higher, respectively. The AlFER sample stimulated by phosphate was 22-fold that of HSF. The morphologies of the resulting Mn-Ferritin from different marine invertebrates were characterized with scanning electron microscopy. Surface morphologies were lamella flower-like and are consistent with changes in surface morphologies of the standard Mn-HSF. Invertebrate recombinant ferritin and HSF both can uptake manganese. We found that the structure of A. leucoproctarecombinant Mn-Ferritin aggregate changed over time. The surface formed lamella flower-like aggregate, but gradually merged to create a relatively uniform plate-like phase of aggregate spherically and fused without clear boundaries.

show abstract

Section: Resultssupporting

confidence: 55%

Section: Resultsmentioning

confidence: 99%

“…japonicus , and A . leucoprocta [36–38]. These three benthic animals are widely distribution in coastal areas of China.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Preparation and Representation of Recombinant Mn-Ferritin Flower-Like Spherical Aggregates from Marine Invertebrates

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…For normalizing the ferritin transcripts, two b-actin specific primers actin-F (59-GGACCTCTACGCCAA-CACTG-39) and actin-R (59-ATGCAAGGATGGAGCCAC-39) were used to amplify an 170 bp fragment. The fluorescent realtime PCR assay was carried out performed in Rotor-Gene 6000real-time PCR detection system and the condition was according to our previous work [12]. The 2…”

Section: Quantitative Expression Analysis Of Ferritinmentioning

confidence: 99%

Phytoremediation of Heavy Metals: A Green Technology

2014

Heavy Metal Contamination of Water and Soil

View full text Add to dashboard Cite

Ferritin, an iron homeostasis protein, has important functions in transition and storage of toxic metal ions. In this study, the full-length cDNA of ferritin was isolated from Dendrorhynchus zhejiangensis by cDNA library and RACE approaches. The higher similarity and conserved motifs for ferritin were also identified in worm counterparts, indicating that it belonged to a new member of ferritin family. The temporal expression of worm ferritin in haemocytes was analyzed by RT-PCR, and revealed the ferritin could be induced by Cd 2+ , Pb 2+ and Fe 2+ . The heavy metal binding activity of recombinant ferritin was further elucidated by atomic force microscopy (AFM). It was observed that the ferritin protein could form a chain of beads with different size against three metals exposure, and the largest one with 35,40 nm in height was identified in the Cd 2+ challenge group. Our results indicated that worm ferritin was a promising candidate for heavy metals detoxification.

show abstract

Developmental Transcriptome Analysis and Identification of Genes Involved in Larval Metamorphosis of the Razor Clam, Sinonovacula constricta

et al. 2016

View full text Add to dashboard Cite

The razor clam Sinonovacula constricta is an important commercial species. The deficiency of developmental transcriptomic data is becoming the bottleneck of further researches on the mechanisms underlying settlement and metamorphosis in early development. In this study, de novo transcriptome sequencing was performed for S. constricta at different early developmental stages by using Illumina HiSeq 2000 paired-end (PE) sequencing technology. A total of 112,209,077 PE clean reads were generated. De novo assembly generated 249,795 contigs with an average length of 585 bp. Gene annotation resulted in the identification of 22,870 unigene hits against the NCBI database. Eight unique sequences related to metamorphosis were identified and analyzed using real-time PCR. The razor clam reference transcriptome would provide useful information on early developmental and metamorphosis mechanisms and could be used in the genetic breeding of shellfish.

show abstract

Identification and characterization of a clam ferritin from Sinonovacula constricta

Cited by 33 publications

References 26 publications

Preparation and Representation of Recombinant Mn-Ferritin Flower-Like Spherical Aggregates from Marine Invertebrates

Preparation and Representation of Recombinant Mn-Ferritin Flower-Like Spherical Aggregates from Marine Invertebrates

Phytoremediation of Heavy Metals: A Green Technology

Developmental Transcriptome Analysis and Identification of Genes Involved in Larval Metamorphosis of the Razor Clam, Sinonovacula constricta

Contact Info

Product

Resources

About