Identification and characterization of a Reeler domain containing protein in Procambarus clarkii provides new insights into antibacterial immunity in crustacean

Comprehensive Proteomics Analysis of the Hemolymph Composition of Sugar-Fed Aedes aegypti Female and Male Mosquitoes

“…In addition to the proteins mentioned above, other noteworthy findings included the presence of proteins with chitin-binding domains that could also be part of recognition, a gelsolin precursor found to be very abundant, as well as the presence of reeler domain-containing proteins (AAEL010139, AAEL024387), annotated as “defense protein 1 and defense protein Hdd11-like” (Table S1). Expression of reeler domain-containing proteins has been shown to be induced by bacterial infection and to be involved in the melanization and nodulation process in some insects and crustaceans. , Also interesting is the presence of a protein (AAEL000860) originally annotated as “unspecified product/unknown” that upon CDD analysis was shown to have a pore forming module (PFM) /aerolysin family domain (cl40431) in the region that spans from residue 253 to 387. Using its Alphafold model (AF-Q17N01–F1) to search the DALI database, in addition to the PFM at the C-terminal region, the N-terminal of this protein shows similarity to natterin and natterin-like proteins (DALI %id of 16% and Z of 15.8 to natterin-3, PDB: 5MH3), which act as sugar recognition motifs.…”

“…Expression of reeler domain-containing proteins has been shown to be induced by bacterial infection and to be involved in the melanization and nodulation process in some insects and crustaceans. 111,112 Also interesting is the presence of a protein (AAEL000860) originally annotated as "unspecified product/ unknown" that upon CDD analysis was shown to have a pore forming module (PFM) /aerolysin family domain (cl40431) in the region that spans from residue 253 to 387. Using its Alphafold model (AF-Q17N01−F1) to search the DALI database, in addition to the PFM at the C-terminal region, the N-terminal of this protein shows similarity to natterin and natterin-like proteins (DALI %id of 16% and Z of 15.8 to natterin-3, PDB: 5MH3), which act as sugar recognition motifs.…”