Identification and characterization of a novel transcript down‐regulated in Dlx1/Dlx2 and up‐regulated in Pax6 mutant telencephalon

Faedo, Andrea; Quinn, Jane C.; Stoney, Patrick N.; Long, Jason E.; Dye, Catherine A.; Zollo, Massimo; Rubenstein, John L.R.; Price, David J.; Bulfone, Alessandro

doi:10.1002/dvdy.20152

Cited by 20 publications

(19 citation statements)

References 38 publications

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“…As has been described previously (Faedo et al, 2004), during the systematic analysis of the TESS transcripts, we found a putative noncoding RNA TESS 31.E5, an ortholog of the rat Evf-1 (AY518691) (Kohtz and Fishell, 2004). Therefore, this confirms the relevance of our approach and the importance of the isolation and characterization of the TESS cDNA library.…”

Section: Discussionsupporting

confidence: 87%

Telencephalic Embryonic Subtractive Sequences: A Unique Collection of Neurodevelopmental Genes

Bulfone¹,

Carotenuto²,

Faedo³

et al. 2005

J. Neurosci.

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The vertebrate telencephalon is composed of many architectonically and functionally distinct areas and structures, with billions of neurons that are precisely connected. This complexity is fine-tuned during development by numerous genes. To identify genes involved in the regulation of telencephalic development, a specific subset of differentially expressed genes was characterized. Here, we describe a set of cDNAs encoded by genes preferentially expressed during development of the mouse telencephalon that was identified through a functional genomics approach. Of 832 distinct transcripts found, 223 (27%) are known genes. Of the remaining, 228 (27%) correspond to expressed sequence tags of unknown function, 58 (7%) are homologs or orthologs of known genes, and 323 (39%) correspond to novel rare transcripts, including 48 (14%) new putative noncoding RNAs. As an example of this latter group of novel precursor transcripts of micro-RNAs, telencephalic embryonic subtractive sequence (TESS) 24.E3 was functionally characterized, and one of its targets was identified: the zinc finger transcription factor ZFP9. The TESS transcriptome has been annotated, mapped for chromosome loci, and arrayed for its gene expression profiles during neural development and differentiation (in Neuro2a and neural stem cells). Within this collection, 188 genes were also characterized on embryonic and postnatal tissue by in situ hybridization, demonstrating that most are specifically expressed in the embryonic CNS. The full information has been organized into a searchable database linked to other genomic resources, allowing easy access to those who are interested in the dissection of the molecular basis of telencephalic development.

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Section: Discussionsupporting

confidence: 87%

Telencephalic Embryonic Subtractive Sequences: A Unique Collection of Neurodevelopmental Genes

Bulfone¹,

Carotenuto²,

Faedo³

et al. 2005

J. Neurosci.

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“…1a). We isolated a full-length cDNA containing ei sequences from a rat embryonic day 15.5 (E15.5) brain library, and found it to be a 3.8-kb alternatively spliced form of Evf-1, an ncRNA previously identified as a downstream target of Shh signaling in the rat embryonic forebrain (Kohtz et al 1998;Faedo et al 2004;Kohtz and Fishell 2004). We named this 3.8-kb, ei-containing alternatively spliced form of Evf-1, Evf-2.…”

Section: Resultsmentioning

confidence: 99%

The Evf-2 noncoding RNA is transcribed from the Dlx-5/6 ultraconserved region and functions as a Dlx-2 transcriptional coactivator

Feng¹,

Bi²,

Clark³

et al. 2006

Genes Dev.

664

567

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The identification of ultraconserved noncoding sequences in vertebrates has been associated with developmental regulators and DNA-binding proteins. One of the first of these was identified in the intergenic region between the Dlx-5 and Dlx-6 genes, members of the Dlx/dll homeodomain-containing protein family. In previous experiments, we showed that Sonic hedgehog treatment of forebrain neural explants results in the activation of Dlx-2 and the novel noncoding RNA (ncRNA), Evf-1. In this report, we show that the Dlx-5/6 ultraconserved region is transcribed to generate an alternatively spliced form of Evf-1, the ncRNA Evf-2. Evf-2 specifically cooperates with Dlx-2 to increase the transcriptional activity of the Dlx-5/6 enhancer in a target and homeodomain-specific manner. A stable complex containing the Evf-2 ncRNA and the Dlx-2 protein forms in vivo, suggesting that the Evf-2 ncRNA activates transcriptional activity by directly influencing Dlx-2 activity. These experiments identify a novel mechanism whereby transcription is controlled by the cooperative actions of an ncRNA and a homeodomain protein. The possibility that a subset of vertebrate ultraconserved regions may function at both the DNA and RNA level to control key developmental regulators may explain why ultraconserved sequences exhibit 90% or more conservation even after 450 million years of vertebrate evolution.

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“…Likewise, Pax6 has been shown to play an important role promoting neurogenesis. In vivo, loss of Pax6 results in neural progenitors having reduced neurogenic potential [24,25], whereas its over-expression in vitro pushes cells towards a neuronal fate [24][25][26]. Altogether, cxcr4, id2, pax3, and pax6 over-expression seem to be responsible for neuronal fate decision.…”

Section: Discussionmentioning

confidence: 99%

Wnt1 and BMP2: two factors recruiting multipotent neural crest progenitors isolated from adult bone marrow

Glejzer

Laudet

Leprince

et al. 2010

Cell. Mol. Life Sci.

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Recent studies have shown that neural crest-derived progenitor cells can be found in diverse mammalian tissues including tissues that were not previously shown to contain neural crest derivatives, such as bone marrow. The identification of those "new" neural crest-derived progenitor cells opens new strategies for developing autologous cell replacement therapies in regenerative medicine. However, their potential use is still a challenge as only few neural crest-derived progenitor cells were found in those new accessible locations. In this study, we developed a protocol, based on wnt1 and BMP2 effects, to enrich neural crest-derived cells from adult bone marrow. Those two factors are known to maintain and stimulate the proliferation of embryonic neural crest stem cells, however, their effects have never been characterized on neural crest cells isolated from adult tissues. Using multiple strategies from microarray to 2D-DIGE proteomic analyses, we characterized those recruited neural crest-derived cells, defining their identity and their differentiating abilities.

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Identification and characterization of a novel transcript down‐regulated in Dlx1/Dlx2 and up‐regulated in Pax6 mutant telencephalon

Cited by 20 publications

References 38 publications

Telencephalic Embryonic Subtractive Sequences: A Unique Collection of Neurodevelopmental Genes

Telencephalic Embryonic Subtractive Sequences: A Unique Collection of Neurodevelopmental Genes

The Evf-2 noncoding RNA is transcribed from the Dlx-5/6 ultraconserved region and functions as a Dlx-2 transcriptional coactivator

Wnt1 and BMP2: two factors recruiting multipotent neural crest progenitors isolated from adult bone marrow

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