Identification and characterization of a novel spermatogenesis related gene LM23 in rat testis

Liu, Meiling; Pei, Kaiyan; Shi, Xiuquan; Liu, Deyu; Jia, Mengmeng

doi:10.1016/j.bbrc.2007.03.024

Cited by 6 publications

(8 citation statements)

References 14 publications

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“…Expression of LM23 mRNA in testis development LM23 is known to be specifically expressed in testis, 3 but its expression in the developing rat testis has not been established. In order to investigate the expression of LM23 in testis development and spermatogenesis, the semiquantitative RT-PCR and real-time PCR were performed to examine the expression of LM23 at different periods of testicular development.…”

Section: Resultsmentioning

confidence: 99%

“…We previously demonstrated that LM23 was one of the genes involved in spermatogenesis. 3 Previously we detected expression of LM23 mRNA by SYBR Greenbased quantitative PCR in type A spermatogonia, pathytene spermatocytes and round spermatids, respectively. The results suggested that the expression level of LM23 was highest in spermatocytes, moderate in round spermatids and very low in spermatogonia.…”

Section: Functional Analysis Of Lm23mentioning

confidence: 99%

“…It has been shown that the LM23 gene is expressed specifically in the testis in a stage-specific manner, and may be involved in rat spermatogenesis, as previous reports from our laboratory have suggested. 3 Cell proliferation, differentiation, senescence and apoptosis are cell cycle-dependent, and the basic regulatory mechanisms in cell cycle progression rely on a multicomponent system. At different phases, progression through the cell cycle is regulated by sequential activation and subsequent inactivation of a series of cyclin-dependent kinases, whose activity depends on interactions with cyclins and cyclindependent kinase inhibitors.…”

Section: Introductionmentioning

confidence: 99%

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Role of LM23 in cell proliferation and apoptosis and its expression during the testis development

Liu¹,

Song²,

Meng³

et al. 2013

Asian J Androl

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LM23, a gene expressed specifically in the testis in a stage-specific manner, has a diverse range of functions that are important in both the life and death of spermatogenic cells. The aim of this study was to further investigate the expression of LM23 in the developing rat testis and the biological function of LM23 in proliferation and antiapoptosis in vitro. Semiquantitative reverse transcription (RT)-PCR and real-time PCR were used to examine the expression of LM23 in testis at different developmental stages. The results suggested that LM23 mRNA levels in the testis increased progressively after birth. The role of LM23 in proliferation was analyzed with cell counting kit-8 (CCK8), colony-forming efficiency (CFE) and flow cytometry assays. The results indicated that ectopic expression of LM23 in 293T cells significantly promoted cell proliferation by increasing cell numbers in S phase. Several methods were used, including CCK8, annexin V and propidium iodide staining and western blotting, to determine the role of LM23 in apoptosis. The results showed that LM23 played a protective role in H2O2-induced apoptosis of 293T cells, mediated at least in part through the Akt/PI3K signal pathway. Taken together, these results provide new insights into the role of LM23 in the development of the testes and spermatogenesis.

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Section: Resultsmentioning

confidence: 99%

Section: Functional Analysis Of Lm23mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Role of LM23 in cell proliferation and apoptosis and its expression during the testis development

Liu¹,

Song²,

Meng³

et al. 2013

Asian J Androl

Self Cite

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“…The total mRNAs from chorionic villi (experimental samples n ¼ 4, control samples n ¼ 5, including the samples were performed microarray analysis) were isolated and the cDNA templates were synthesised as described previously [6]. A cDNA sample expressing the target gene was selected as template to amplify a target gene segment by conventional PCR.…”

Section: Real-time Rt-pcrmentioning

confidence: 99%

Differentially expressed genes in early gestational chorionic villi after administration of mifepristone

Liu

Shi

Jia

2010

Gynecological Endocrinology

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To investigate the effect of mifepristone on gene expression of human chorionic villi in early pregnancy, nine women were recruited into a randomised controlled trial. All subjects were healthy women who had regular menstrual cycles and sought termination of pregnancy up to 40 days gestational age. In the test group, gestational sacs were taken by vacuum aspiration of the uterus 24 h after a single dose of 150 mg mifepristone was administered. Chorionic villi were collected and frozen in liquid nitrogen. The control samples were collected using the same method from the women without administration of mifepristone. The gene expressions of villus were monitored by human cDNA microarrays. It is found that the expressions of 262 transcripts were significantly altered in the test group. Gene ontology and pathways analyses were conducted to further analyse these genes. Many of these genes are known to play potentially an important role in the placentation and the molecular regulation of maternal-fetal interface. Therefore, it is suggested that the placental development and microenvironment of the maternal-fetal interface were interfered by administration of mifepristone. These data provide insight into the molecular mechanism about medical abortion induced by mifepristone.

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“…These proteins participate in the cell cycle, DNA damage response and entry into meiosis, through direct binding to CDK1/2. Jai and colleagues originally identified LM23 as a gene by differential display that was very highly expressed in rat testis in the cells that are undergoing meiosis, spermatocytes 8 . In this report, they demonstrated that LM23 has a Speedy/RINGO box that is 97% homologous to the mouse Speedy/RINGO A.…”

mentioning

confidence: 99%

A new Speedy/RINGO protein may help regulate male meiosis

Yamazaki

Ward²

2011

Asian J Androl

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Identification and characterization of a novel spermatogenesis related gene LM23 in rat testis

Cited by 6 publications

References 14 publications

Role of LM23 in cell proliferation and apoptosis and its expression during the testis development

Role of LM23 in cell proliferation and apoptosis and its expression during the testis development

Differentially expressed genes in early gestational chorionic villi after administration of mifepristone

A new Speedy/RINGO protein may help regulate male meiosis

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